4 documents found, page 1 of 1
Efficiency of purification methods on the recovery of exopolysaccharides from f...
Pintado, Ana I. E.; Ferreira, José A.; Pintado, Manuela M. E.; Gomes, Ana M. P.; Malcata, F. Xavier; Coimbra, Manuel A.De-Man Rogosa and Sharpe (MRS) is a complex medium commonly used to obtain exopolysaccharides (EPS) from lactic acid bacteria. However, the various nutrients (carbon and nitrogen sources) of media and supplements added to enhance the bacterial growth and EPS production, may interfere with the purification of the extracts resulting in an over-estimation of the EPS and erroneous structural assignments. The effici...
Novel eco-friendly method to extract keratin from hair
Cassoni, Ana C.; Freixo, Ricardo; Pintado, Ana I. E.; Amorim, Manuela; Pereira, Carlos D.; Madureira, Ana Raquel; Pintado, Manuela M. E.Pig slaughtering for meat production is a major worldwide industry that generates many byproducts that can cause environmental problems. Nonetheless, it is possible to have a management of these residues toward the reuse and valorization of these byproducts. One opportunity focuses on the extraction of value-added compounds such as keratin since pig hair is composed by 80% of this protein. There are some method...
Bacteria immobilsation on hydroxyapatite surface for wastewater treatment
Pereira, Sofia Isabel Almeida; Piccirillo, Clara; Marques, Ana P. G. C.; Pullar, Robbert C.; Pintado, Manuela M. E.; Castro, Paula M. L.Heavy metals pollution in the environment is a big cause of concern, due to the toxicity of the metals themselves. In this work we propose an innovative method for heavy metals removal from contaminated waste water – a combination of hydroxyapatite (already used for water treatment) and microorganisms. Selected bacterial strains were immobilised on the surface of hydroxyapatite of natural origin (extracted from...
In vitro evaluation of “horchata” co-products as carbon source for probiotic ba...
Sánchez-Zapata, Elena; Fernández-López, Juana; Pérez-Alvarez, José A.; Soares, José; Sousa, Sérgio; Gomes, Ana M. P.; Pintado, Manuela M. E.Tiger nut milk ("horchata") liquid co-products (TNLC) were evaluated as carbon source for probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium animalis) growth by screening via microplate assay and determination of viable cells and metabolic activity. Based on MRS five different basal media were prepared ((i) without carbon source, (ii) with 2% (w/v) glucose, (iii) with 2% (w/v) FOS, (iv) with 2% (...