7 documents found, page 1 of 1
The chick chorioallantoic membrane model: A new in vivo tool to evaluate breast...
Pinto, MT; Ribeiro, AS; Conde, I; Carvalho, R; Paredes, JThe high plasticity of cancer stem-like cells (CSCs) allows them to differentiate and proliferate, specifically when xenotransplanted subcutaneously into immunocompromised mice. CSCs are highly tumorigenic, even when inoculated in small numbers. Thus, in vivo limiting dilution assays (LDA) in mice are the current gold standard method to evaluate CSC enrichment and activity. The chick embryo chorioallantoic memb...
Neuroprotection in early stages of Alzheimer’s disease is promoted by transthyr...
Gião, T; Saavedra, J; Vieira, JR; Pinto, MT; Arsequell, G; Cardoso, IBackground: While still controversial, it has been demonstrated that vascular defects can precede the onset of other AD hallmarks features, making it an important therapeutic target. Given that the protein transthyretin (TTR) has been established as neuroprotective in AD, here we investigated the influence of TTR in the vasculature. Methods: We evaluated the thickness of the basement membrane and the length of ...
The extracellular small leucine-rich proteoglycan biglycan is a key player in g...
Pinto, F; Santos-Ferreira, L; Pinto, MT; Gomes, C; Reis, CABiglycan (BGN gene), an extracellular proteoglycan, has been described to be associated with cancer aggressiveness. The purpose of this study was to clarify the clinical value of biglycan as a biomarker in multiple independent GC cohorts and determine the in vitro and in vivo role of biglycan in GC malignant features. We found that BGN is commonly over-expressed in all analyzed cohorts, being associated with di...
BR-BCSC Signature: The Cancer Stem Cell Profile Enriched in Brain Metastases th...
Dionísio, MR; Vieira, AF; Carvalho, R; Conde, I; Oliveira, M; Gomes, M; Pinto, MT; Pereira, P; Pimentel, J; Souza, C; Marques, MMC; Silva, VDBrain metastases remain an unmet clinical need in breast oncology, being frequently found in HER2-overexpressing and triple-negative carcinomas. These tumors were reported to be highly cancer stem-like cell-enriched, suggesting that brain metastases probably arise by the seeding of cancer cells with stem features. Accordingly, we found that brain-tropic breast cancer cells show increased stem cell activity and ...
The antibacterial and angiogenic effect of magnesium oxide in a hydroxyapatite ...
Coelho, CC; Padrão, T; Costa, L; Pinto, MT; Costa, PC; Domingues, VF; Quadros, PA; Monteiro, FJ; Sousa, SRBone graft infections are serious complications in orthopaedics and the growing resistance to antibiotics is increasing the need for antibacterial strategies. The use of magnesium oxide (MgO) is an interesting alternative since it possesses broad-spectrum antibacterial activity. Additionally, magnesium ions also play a role in bone regeneration, which makes MgO more appealing than other metal oxides. Therefore,...
O-glycans truncation modulates gastric cancer cell signaling and transcription ...
Freitas, D; Campos, D; Gomes, J; Pinto, F; Macedo, JA; Matos, R; Mereiter, S; Pinto, MT; Polónia, A; Gärtner, F; Magalhães, A; Reis, CABackground: Changes in glycosylation are known to play critical roles during gastric carcinogenesis. Expression of truncated O-glycans, such as the Sialyl-Tn (STn) antigen, is a common feature shared by many cancers and is associated with cancer aggressiveness and poor-prognosis. Methods: Glycoengineered cell lines were used to evaluate the impact of truncated O-glycans in cancer cell biology using in vitro fun...
Ionizing radiation modulates human macrophages towards a pro-inflammatory pheno...
Pinto, AT; Pinto, ML; Cardoso, AP; Monteiro, C; Pinto, MT; Maia, AF; Castro, P; Figueira, R; Monteiro, A; Marques, M; Mareel, M; Santos, S; Seruca, RIn order to improve the efficacy of conventional radiotherapy, attention has been paid to immune cells, which not only modulate cancer cell response to therapy but are also highly recruited to tumours after irradiation. Particularly, the effect of ionizing radiation on macrophages, using therapeutically relevant doses, is not well understood. To evaluate how radiotherapy affects macrophage behaviour and macroph...