3 documents found, page 1 of 1
Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion...
Soares, Maria; Pinto, Maria M.; Nobre, Rui J.; Almeida, Luís Pereira de; Rasteiro, Maria da Graça; Santos, Teresa Almeida; Santos, João RamalhoFollicular fluid (FF) is the microenvironment where a growing oocyte develops. Intrafollicular communication ensures oocyte competence and is carried out through paracrine signaling, the exchange of molecules via gap junctions, and the trafficking of extracellular vesicles (EVs). The study of FF-derived EVs is important for both translational and fundamental research in the female reproductive field. This study...
A standardised protocol for blood and cerebrospinal fluid collection and proces...
Santana, Magda M.; Gaspar, Laetitia S.; Pinto, Maria M.; Silva, Patrick Joel da; Adão, Diana; Pereira, Dina; Ribeiro, Joana Afonso; Cunha, InêsThe European Spinocerebellar Ataxia Type 3/Machado-Joseph Disease Initiative (ESMI) is a consortium established with the ambition to set up the largest European longitudinal trial-ready cohort of Spinocerebellar Ataxia Type 3/Machado-Joseph Disease (SCA3/MJD), the most common autosomal dominantly inherited ataxia worldwide. A major focus of ESMI has been the identification of SCA3/MJD biomarkers to enable futur...
Simple and Fast SEC-Based Protocol to Isolate Human Plasma-Derived Extracellula...
Gaspar, Laetitia da Silva; Santana, Magda M.; Henriques, Carina; Pinto, Maria M.; Ribeiro-Rodrigues, Teresa M.; Girão, Henrique; Nobre, Rui JorgeExtracellular vesicles (EVs) are membranous structures that protect RNAs from damage when circulating in complex biological fluids, such as plasma. RNAs are extremely specific to health and disease, being powerful tools for diagnosis, treatment response monitoring, and development of new therapeutic strategies for several diseases. In this context, EVs are potential sources of disease biomarkers and promising d...