49 documents found, page 1 of 5
Quantitative PCR assays as a monitoring tool for bacterial genera in fresh fish...
Pinto, Patricia IS; NAJAFPOUR, BABAK; Lima, Pedro; P. Machado; Aires, Tania; Engelen, Aschwin; Tsironi, T.; Anjos Guerreiro, Liliana Isabel ToméFresh fish fillets are a valuable but highly perishable food, and their rapid microbial deterioration is a drawback for food safety and sustainability of aquaculture, food and retail industries. Quantitative PCR (qPCR) assays based on 16S rRNA gene (16S) sequences were developed for the most abundant bacteria genera detected by metagenomics in fresh or processed fish fillets. The efficiency and specificity of s...
O Micro e a poluição marinha
Pinto, Patricia IS; Duarte, Pedro; Rocha, CarlosO livro “Micro e a Poluição Marinha” nasceu no âmbito dos projetos MicroWaste (premiado Mares Circulares CocaCola, 2023) e MicroFish (bolsa FAM Foundation, 2024) da investigadora Patrícia Pinto (Centro de Ciências do Mar - CCMAR / Universidade do Algarve) - dedicados à monitorização de poluentes ambientais e avaliação dos seus impactos na vida marinha na Região do Algarve - e da sua ambição de os aliar a ativid...
Gene transcripts responsive to drought stress identified in Citrus macrophylla ...
da Silva, Melina; Pinto, Patricia IS; Guerra, Rui Manuel Farinha das Neves; Duarte, Amilcar; Power, Deborah; Marques, N T.Citrus macrophylla Wester (CM) has valuable agronomic characteristics such as the ability to grow in saline soils, although with low tolerance to prolonged drought stress (DS). To understand the mechanisms that characterize CM response to water scarcity, this study compared transcriptome profile changes in CM stem tissue when exposed to DS and identified a total of 2745 differentially expressed transcripts (DET...
Core microbiome profiles and their modification by environmental, biological, a...
Najafpour, Babak; Pinto, Patricia IS; Sanz, Eric Climent; Martinez-Blanch, Juan F.; Canario, Adelino; Moutou, Katerina A.; Power, Deborah16S rRNA gene sequencing and bacteria-and genus-specific quantitative PCR was used to profile microbial communities and their associated functions in water, live feed (microalgae, Artemia, and rotifer), and European sea bass and gilthead sea bream larvae from hatcheries in Greece and Italy. The transfer to larvae of genus containing potential pathogens of fish was more likely with Artemia and rotifer than with ...
Proteomics of sea bass skin-scales exposed to the emerging pollutant fluoxetine...
Anjos, L.; Estêvão, Dulce; Santos, S.; Santa, C.; Manadas, B.; Monsinjon, T.; Canario, Adelino; Power, Deborah; Pinto, Patricia ISTeleost fish skin-scales are essential for protection and homeostasis and the largest tissue in direct contact with the environment, but their potential as early indicators of pollutant exposure are hampered by limited knowledge about this model. This study evaluated multi-level impacts of in vivo exposure of European sea bass to fluoxetine (FLX, a selective serotonin-reuptake inhibitor and an emerging pollutan...
Quantifying dominant bacterial genera detected in metagenomic data from fish eg...
Najafpour, Babak; Canario, Adelino; Power, Deborah; Pinto, Patricia ISThe goal of this study was to design genus-specific primers for rapid evaluation of the most abundant bacterial genera identified using amplicon-based sequencing of the 16S rRNA gene in fish-related samples and surrounding water. Efficient genus-specific primers were designed for 11 bacterial genera including Alkalimarinus, Colwellia, Enterovibrio, Marinomonas, Massilia, Oleispira, Phaeobacter, Photobacterium, ...
Proteomic changes in the extracellular environment of sea bass thymocytes expos...
Moreira, Catarina; Hétru, Julie; Paiola, Matthieu; Duflot, Aurélie; Chan, Philippe; Vaudry, David; Monsinjon, Tiphaine; Knigge, ThomasThe thymus is an important immune organ providing the necessary microenvironment for the development of a diverse, self-tolerant T cell repertoire, which is selected to allow for the recognition of foreign antigens while avoiding self-reactivity. Thymus function and activity are known to be regulated by sex steroid hormones, such as oestrogen, leading to sexual dimorphisms in immunocompetence between males and ...
Factors driving bacterial microbiota of eggs from commercial hatcheries of Euro...
Najafpour, Babak; Pinto, Patricia IS; Moutou, Katerina A.; Canario, A.V.M.; Power, Deborah MaryA comprehensive understanding of how bacterial community abundance changes in fishes during their lifecycle and the role of the microbiota on health and production is still lacking. From this perspective, the egg bacterial communities of two commercially farmed species, the European seabass (Dicentrarchus labrax) and the gilthead seabream (Sparus aurata), from different aquaculture sites were compared, and the ...
Disclosing proteins in the leaves of cork oak plants associated with the immune...
Coelho, Ana Cristina; Pires, Rosa; Schütz, Gabriela; Santa, Cátia; Manadas, Bruno; Pinto, Patricia ISThe pathological interaction between oak trees and Phytophthora cinnamomi has implications in the cork oak decline observed over the last decades in the Iberian Peninsula. During host colonization, the phytopathogen secretes effector molecules like elicitins to increase disease effectiveness. The objective of this study was to unravel the proteome changes associated with the cork oak immune response triggered b...
Trichome density in relation to volatiles emission and 1,8-Cineole synthase gen...
Marques, N T.; Filipe, Alexandra; Barroso, Jose; Trindade, Helena; Power, Deborah; Figueiredo, Ana Cristina; Pinto, Patricia IS1,8-Cineole is the main volatile produced by Thymus albicans Hoffmanns. & Link 1,8-cineole chemotype. To understand the contribution of distinct plant organs to the high 1,8-cineole production, trichome morphology and density, as well as emitted volatiles and transcriptional expression of the 1,8-cineole synthase (CIN) gene were determined separately for T. albicans leaves, bracts, calyx, corolla and infloresce...