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Clinical and epidemiological profiles of patients with American cutaneous leish...
Melo,Maria Gabriella Nunes de; Morais,Rayana Carla Silva de; Goes,Tayná Correia de; Silva,Rômulo Pessoa e; Morais,Rômulo Freire deAbstract INTRODUCTION: Brazil has a high number of cases of American cutaneous leishmaniasis (ACL) in the north and northeast regions. Therefore, continuous surveillance of environmental and socioeconomic factors in endemic areas is needed to develop strategic control measures. This study aimed to describe the clinical and epidemiological profiles of patients with ACL. METHODS: All patients were from the states...
Standardization and evaluation of a duplex real-time quantitative PCR for the d...
Trajano-Silva,Lays Adrianne Mendonça; Pessoa-e-Silva,Rômulo; Gonçalves-de-Albuquerque,Suênia da Cunha; Morais,Rayana Carla Silva deAbstract INTRODUCTION: Molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as the leishmaniases. The present study describes the optimization and evaluation of a duplex real-time quantitative PCR (qPCR) protocol developed for the simultaneous detection of Leishmania infantum DNA and sample quality control. METHODS: After preliminar...
Tracking false-negative results in molecular diagnosis: proposal of a triplex-P...
Gonçalves-de-Albuquerque,Suênia da Cunha; Silva,Rômulo Pessoa e; Morais,Rayana Carla Silva de; Trajano-Silva,Lays Adrianne MendonçaBackground : Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect the sensitivity of PCR assays, including DNA yield and purity, an optimal extraction method is pivotal. Losses of a parasite’s DNA during extraction may significantly impair its detection by PCR and lead to false-negative results...