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BiLaWO6: Er3+/Tm3+/Yb3+ phosphor: study of multiple fluorescence intensity rati...

Pavani, K.; Neves, A. J.; Pinto, Ricardo J. B.; Freire, Carmen S. R.; Soares, M. J.; Graça, M. P. F.; Kumar, K. Upendra; Jakka, S.K.


Bacterial nanocellulose-hyaluronic acid microneedle patches for skin applicatio...

Fonseca, Daniela F. S.; Vilela, Carla; Pinto, Ricardo J. B.; Bastos, Verónica; Oliveira, Helena; Catarino, José; Faísca, Pedro; Rosado, Catarina

The aim of the present study was to develop innovative patches for dermo-cosmetic applications based on dissolvable hyaluronic acid (HA) microneedles (MNs) combined with bacterial nanocellulose (BC) as the back layer. HA was employed as an active biomacromolecule, with hydrating and regenerative properties and volumizing effect, whereas BC was used as support for the incorporation of an additional bioactive mol...


Multifunctional hybrid structures made of open-cell aluminum foam impregnated w...

Pinto, Susana C.; Silva, Nuno H. C. S.; Pinto, Ricardo J. B.; Freire, Carmen S. R.; Duarte, Isabel; Vicente, Romeu; Vesenjak, Matej

This work focuses on exploring combinations of disintegrated bacterial cellulose nanofibres (BC) with graphene oxide (GO) (reduced and non-reduced) and phase change materials (PCMs) prepared in the form of foam-like structures. The presence of GO remarkable improves the fire-retardancy and provides dimensional stability to the foams while PCMs gives thermal energy storage capacity. The foams were exposed to met...


Tuning lysozyme nanofibers dimensions using deep eutectic solvents for improved...

Silva, Nuno H. C. S.; Vilela, Carla; Pinto, Ricardo J. B.; Martins, Manuel A.; Marrucho, Isabel M.; Freire, Carmen S. R.

Deep eutectic solvents (DESs), a novel generation of solvents, have recently been described as efficient and timesaving fibrillation agents for proteins. In this context, the present work aims at assessing the effect of the hydrogen bond donor (HBD) of cholinium chloride ([Ch]Cl):carboxylic acid based DESs on the dimensions (length and width) of lysozyme nanofibers (LNFs). Mono-, di- and tri-carboxylic acids (a...


Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions

Silva, Nuno H. C. S.; Pinto, Ricardo J. B.; Freire, Carmen S. R.; Marrucho, Isabel M.

Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a ne...


Timesaving microwave assisted synthesis of insulin amyloid fibrils with enhance...

Carvalho, Tiago; Pinto, Ricardo J. B.; Martins, Manuel A.; Silvestre, Armando J. D.; Freire, Carmen S. R.

Insulin amyloid fibrils with enhanced aspect ratio, were prepared using a timesaving microwave assisted (MW) methodology, reducing the incubation time from 13 to 2 h. The fibrillation process was followed indirectly by Thioflavin T Fluorescence and UV-vis analysis, by measuring the amount of beta-sheets formed and the insulin present in solution, respectively. TEM and AFM analysis revealed that the insulin fibr...


Fluorescent Bioactive Corrole Grafted-Chitosan Films

Barata, Joana F. B.; Pinto, Ricardo J. B.; Vaz Serra, Vanda I. R. C.; Silvestre, Armando J. D.; Trindade, Tito; Neves, Maria Graca P. M. S.

Transparent corrole grafted-chitosan films were prepared by chemical modification of chitosan with a corrole macrocycle, namely, 5,10,15-tris(pentafluorophenyl)corrole (TPFC), followed by solvent casting. The obtained films were characterized in terms of absorption spectra (UV-vis), FLIM (fluorescence lifetime imaging microscopy), structure (FTIR, XPS), thermal stability (TGA), thermomechanical properties (DMA)...


One-pot synthesis of biofoams from castor oil and cellulose microfibers for ene...

Sousa, Andreia F.; Matos, Marina; Pinto, Ricardo J. B.; Freire, Carmen S. R.; Silvestre, Armando J. D.

The use of renewable feedstocks in foam technology has created a worldwide demand for more sustainable materials. Castor oil is a vegetable oil, composed mainly of triricinoglycerol, a natural polyol, suitable for polyurethane foam production. In this study, castor oil and variable amounts of microcrystalline cellulose (MCC) fibers were used in a straightforward one-pot synthesis approach for the preparation of...


Unveiling the Chemistry behind the Green Synthesis of Metal Nanoparticles

Santos, Sonia A. O.; Pinto, Ricardo J. B.; Rocha, Silvia M.; Marques, Paula A. A. P.; Neto, Carlos Pascoal; Silvestre, Armando J. D.

Nanobiotechnology has emerged as a fundamental domain in modern science, and metallic nanoparticles (NPs) are one of the largest classes of NPs studied because of their wide spectrum of possible applications in several fields. The use of plant extracts as reducing and stabilizing agents in their synthesis is an interesting and reliable alternative to conventional methodologies. However, the role of the differen...


Cationic release behaviour of antimicrobial cellulose/silver nanocomposites

Silva, Fabio M.; Pinto, Ricardo J. B.; Daniel-da-Silva, Ana L.; Trindade, Tito

Silver nanoparticles (NPs) have received great attention, mainly due to their application as antimicrobial agents in diverse products, including textile- and paper-based materials. In this context, straightforward methodologies to monitor their cationic silver release capacity in diverse environments are required due to the rise of manufactured products containing silver NPs. Here, we describe the application o...


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