8 documents found, page 1 of 1
The pro-proliferative effect of insulin in human breast epithelial DMBA-transfo...
Silva, C; Andrade, N; Guimaraes, JT; Cardoso, E; Meireles, C; Pinto, V; Paiva, J; Martel, FType 2 diabetes mellitus (T2DM) is linked to an increased risk of breast cancer. We aimed to investigate how T2DM-associated characteristics (high levels of glucose, insulin, leptin, inflammatory mediators and oxidative stress) influence breast cancer carcinogenesis, in DMBA-treated (MCF-12ADMBA) and non-treated breast epithelial (MCF-12A) cell lines. Insulin (50 nM) promotes cell proliferation, 3H-DG uptake an...
Isolation Method and Characterization of Outer Membranes Vesicles of Helicobact...
Melo, J; Pinto, V; Fernandes, T; Malheiro, AR; Osorio, H; Figueiredo, C; Leite, MOuter membrane vesicles (OMVs) are small vesicles constitutively shed by all Gram-negative bacterium, which have been proposed to play a role in Helicobacter pylori persistence and pathogenesis. The methods currently available for the isolation of H. pylori OMVs are diverse and time-consuming, raising the need for a protocol standardization, which was the main aim of this study. Here, we showed that the chemica...
Forecasting COVID-19 Severity by Intelligent Optical Fingerprinting of Blood Sa...
Faria, SP; Carpinteiro, C; Pinto, V; Rodrigues, SM; Alves, J; Marques, F; Lourenço, M; Santos, PH; Ramos, A; Cardoso, MJ; Guimarães, JT; Rocha, SForecasting COVID-19 disease severity is key to supporting clinical decision making and assisting resource allocation, particularly in intensive care units (ICUs). Here, we investigated the utility of time- and frequency-related features of the backscattered signal of serum patient samples to predict COVID-19 disease severity immediately after diagnosis. ICU admission was the primary outcome used to define dise...
Evaluation of the use of formalin-fixed and paraffin- embedded archive gastric ...
Pinto-Ribeiro, I; Ferreira, RM; Pereira-Marques, J; Pinto, V; Macedo, G; Carneiro, F; Figueiredo, CLarge numbers of well-characterized clinical samples are fundamental to establish relevant associations between the microbiota and disease. Formalin-fixed and paraffin-embedded (FFPE) tissues are routinely used and are widely available clinical materials. Since current approaches to study the microbiota are based on next-generation sequencing (NGS) targeting the bacterial 16S rRNA gene, our aim was to evaluate ...
TERT promoter mutation as a potential predictive biomarker in BCG-treated bladd...
Batista, R; Lima, L; Vinagre, J; Pinto, V; Lyra, J; Máximo, V; Santos, L; Soares, PTelomerase reverse transcriptase gene promoter (TERTp) mutations are recognized as one of the most frequent genetic events in bladder cancer (BC). No studies have focused on the relevance of TERTp mutations in the specific group of tumors treated with Bacillus Calmette–Guérin (BCG) intravesical therapy. Methods — 125 non muscle invasive BC treated with BCG therapy (BCG-NMIBC) were screened for TERTp mutations, ...
Multiple myeloma: Available therapies and causes of drug resistance
Pinto, V; Bergantim, R; Caires, HR; Seca, H; Guimarães, JE; Vasconcelos, MHMultiple myeloma (MM) is the second most common blood cancer. Treatments for MM include corticosteroids, alkylating agents, anthracyclines, proteasome inhibitors, immunomodulatory drugs, histone deacetylase inhibitors and monoclonal antibodies. Survival outcomes have improved substantially due to the introduction of many of these drugs allied with their rational use. Nonetheless, MM patients successively relaps...
Glycans as key checkpoints of T cell activity and function
Pereira, MS; Alves, I; Vicente, M; Campar, A; Silva, MC; Padrão, NA; Pinto, V; Fernandes, Â; Dias, AM; Pinho, SSThe immune system is highly controlled and fine-tuned by glycosylation, through the addition of a diversity of carbohydrates structures (glycans) to virtually all immune cell receptors. Despite a relative backlog in understanding the importance of glycans in the immune system, due to its inherent complexity, remarkable findings have been highlighting the essential contributions of glycosylation in the regulatio...
Frequency of TERT promoter mutations in human cancers
Vinagre, J; Almeida, A; Pópulo, H; Batista, R; Lyra, J; Pinto, V; Coelho, R; Celestino, R; Prazeres, H; Lima, L; Melo, M; Rocha, AG; Preto, A; Castro, PReactivation of telomerase has been implicated in human tumorigenesis, but the underlying mechanisms remain poorly understood. Here we report the presence of recurrent somatic mutations in the TERT promoter in cancers of the central nervous system (43%), bladder (59%), thyroid (follicular cell-derived, 10%) and skin (melanoma, 29%). In thyroid cancers, the presence of TERT promoter mutations (when occurring tog...