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Purification of a PHA-like chitin-binding protein from Acacia farnesiana seeds ...

Santi-Gadelha, Tatiane; Rocha, Bruno Anderson Matias; Oliveira, Cecília Carvalho; Aragão, Karoline Sabóia; Marinho, EEmmanuel Silva

A lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) followed by chromatofocusing. AFAL agglutinated rabbit erythrocytes and did not agglutinate human ABO...

Date: 2013   |   Origin: Oasisbr
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