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Purification, partial characterization and immobilization of a mannose-specific...

Pinto Júnior, Vanir Reis; Santiago, Mayara Queiroz de; Osterne, Vinícius José da Silva; Correia, Jorge Luis Almeida

Lectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-Dmannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, 12,998 ± 1 and 12,...

Date: 2022   |   Origin: Oasisbr

Análise da estrutura cristalográfica de DLyl, uma lectina de sementes de Diocle...

Pinto Júnior, Vanir Reis

Lectins are a class of carbohydrate-binding proteins or glycoproteins with diverse specificities and functions. The determination and characterization of the three-dimensional structures of these proteins are keys to understanding their biological effects. Recent studies have explored the anticancer potential of Diocleinae lectins (from Leguminoseae family), evaluating their antiproliferative effect and their a...

Date: 2019   |   Origin: Oasisbr

Análise estrutural de uma lectina de sementes de Diocle lasiophylla Mart. ex Be...

Pinto Júnior, Vanir Reis

PINTO JÚNIOR, Vanir Reis. Análise estrutural de uma lectina de sementes de Diocle lasiophylla Mart. ex Benth e avaliação do seu efeito vasorelaxante, inflamatório e nociceptivo. 2016. 111 f. Dissertação (Mestrado em Biotecnologia de Recursos Naturais)-Universidade Federal do Ceará, 2016.; Lectins are (glyco)proteins of non-immune origin, capable of reversibly binding to specific mono or oligosaccharides, via a ...

Date: 2016   |   Origin: Oasisbr

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