Lectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-Dmannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, 12,998 ± 1 and 12,...
Lectins are a class of carbohydrate-binding proteins or glycoproteins with diverse specificities and functions. The determination and characterization of the three-dimensional structures of these proteins are keys to understanding their biological effects. Recent studies have explored the anticancer potential of Diocleinae lectins (from Leguminoseae family), evaluating their antiproliferative effect and their a...
PINTO JÚNIOR, Vanir Reis. Análise estrutural de uma lectina de sementes de Diocle lasiophylla Mart. ex Benth e avaliação do seu efeito vasorelaxante, inflamatório e nociceptivo. 2016. 111 f. Dissertação (Mestrado em Biotecnologia de Recursos Naturais)-Universidade Federal do Ceará, 2016.; Lectins are (glyco)proteins of non-immune origin, capable of reversibly binding to specific mono or oligosaccharides, via a ...