45 documents found, page 1 of 5
Bacteriophages to tackle Alzheimer´s disease
Martins, Ivone M.; Lima, Alexandre Ferreira; Oliveira, Tiago Gil; Azeredo, Joana; Kessels, Helmut W.The first stages of Alzheimers disease (AD) pathology involve the formation of toxic amyloid beta (A) oligomers, which can begin to form up to two decades before symptoms and other known disease markers appear. The ability to detect A oligomers would allow early diagnosis, leading to improved disease management, care and prognosis through the use of therapeutic strategies targeting A before irreparable brain da...
M13 bacteriophages displaying peptide motifs targeting amyloid- eta, methods an...
Martins, Ivone M.; Azeredo, Joana; Kessels, Helmut W.; Gomes, Cláudio M.; Cristóvão, Joana S.The present disclosure relates to an engineered M13 bacteriophage displaying amyloidogenic peptide motifs from amyloid beta 42 (A42) at its surface. The present disclosure further relates to the use of the disclosed engineered M13 bacteriophage for detecting early species of A, namely oligomeric and fibrillar A, and preventing its aggregation promoting the inhibition of the progression of Alzheimer's disease an...
M13 phage grafted with peptide motifs as a tool to detect amyloid- oligomers in...
Martins, Ivone M.; Lima, Alexandre; de Graaff, Wim; Cristóvão, Joana S.; Brosens, Niek; Aronica, Eleonora; Kluskens, Leon; Gomes, Cláudio M.Oligomeric clusters of amyloid- (A) are one of the major biomarkers for Alzheimers disease (AD). However, proficient methods to detect A-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying A-derived peptides on their surface preferentially interact with A-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-...
Bacteriophages a bio sustainable solution to tackle Alzheimer´s disease
Lima, Alexandre; de Graaff, Wim; Cristóvão, Joana S.; Brosens, Niek; Aronica, Eleonora; Gomes, Cláudio M.; Azeredo, Joana; Kessels, HelmutIntroduction: Amyloid-beta (AB) is a prime suspect to cause Alzheimers disease (AD), an irreversible, progressive and age-dependent neurodegenerative disorder affecting millions of people worldwide. An accumulation of AB in the brain leads to its aggregation into soluble oligomeric and fibrillar clusters, which are the culprits to impair synaptic function and memory formation in mice models. Currently, we lack ...
Detection of amyloid-beta oligomers in early Alzheimer´s disease
Lima, Alexandre; de Graaff, Wim; Aronica, Eleonora; Azeredo, Joana; Kessels, Helmut W.; Martins, Ivone M.Amyloid-beta (A) seems to play a central role in Alzheimers disease (AD) pathology. During disease progression A peptides aggregate into various structures including soluble oligomers and fibrils, which ultimately deposit in the brain as insoluble plaques. While the plaques are a well-known hallmark of AD, recent findings revealed that even before plaque formation the still-soluble A oligomers are already able ...
Phage display identified peptide with selectivity for human osteoarthritic chon...
Martins, Ivone M.; Canadas, Raphaël F.; Pereira, Hélder; Azeredo, Joana; Reis, R. L.; Oliveira, J. M.; Azevedo, Helena S.Osteoarthritis (OA) is one of the most common joint disorders in western populations, affecting millions of people worldwide and with a rising incidence as life expectancy continues to increase. Current therapies for OA management fail to halt the progressive degradation of articular cartilage, urging the need for more effective therapies to improve cartilage function and enhance patient's quality of life. Thro...
Phage display an old resource for new applications
Martins, Ivone M.Phage Display is a very versatile technique not only used for the identification of molecules and receptors but also for the use of bacteriophages as scaffolds to display a given molecule of interest for targeted delivery of drugs for instance. This talk will provide some of the main applicatons of Phage Display.
Viruses of Microbes: the latest conquests
Oliveira, Ana; São-José, Carlos; Pires, Diana Priscila Penso; Oliveira, Hugo Alexandre Mendes; Martins, Ivone M.; Azeredo, Joana; Dabrowska, KrystynaThis book covers abstracrs from the most recent advances in ecology and evolution of microbial viruses, virus structures and function, virus-host interaction, agro-food, veterinary and environmental biotechnology applications and phage therapy.
A phage-based platform to detect amyloid-beta oligomers
Lima, Alexandre; de Graaff, Wim; Aronica, Eleonora; Martins, Ivone M.; Azeredo, Joana; Kessels, HelmutAmyloid-beta (A) is a prime suspect to cause Alzheimers disease (AD). A peptides can aggregate into soluble oligomers and fibrils, to finally deposit in insoluble plaques. Even though the plaques are main hallmarks of Alzheimers, before plaque formation, the still-soluble A oligomers and fibrils are the culprits that trigger a loss in synapses and memory dysfunction. However, whereas we are well-able to identif...
Bacteriophages as a tool to tackle Alzheimer´s Disease
Martins, Ivone M.Amyloid-beta (AB) is a prime suspect to cause Alzheimers disease (AD), an irreversible, progressive and age-dependent neurodegenerative disorder affecting millions of people worldwide. An accumulation of AB in the brain leads to its aggregation into soluble oligomeric and fibrillar clusters, which are the culprits to impair synaptic function and memory formation in mice models. Currently, we lack diagnostic too...