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Serological evidence of West Nile Virus infection in horses from the municipali...

Loureiro, Filipa; Pires, Humberto; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Santos Pintado, Cristina Maria Baptista

West Nile Virus (WNV) is an RNA virus from the family Flaviviridae. It is transmitted by several genera of mosquitoes, with Cullex spp. as the main vectors identified in Europe and North America. Birds are amplifying agents and important to the transmission cycle, during the viremia period. Equines are considered dead-ends hosts and are recognized as sentinels of the virus in many countries.


Evidence for sporadic Coxiella burnetii excretion in sheep milk, central Portugal

Pires, Humberto; Silva, S. Santos; Cruz, A.; Cardoso, Luís; Lopes, A.P.; Pereira, M.A.; Nóbrega, C.; Mega, A.C.; Santos, C.; Cruz, R.; Esteves, F.

Coxiella burnetii, the causative agent of Q fever, is a globally distributed zoonosis, with cattle, sheep, and goats considered the main reservoirs of the disease (Agger et al,., 2013; Bento et al., 2023). Human transmission primarily occurs through inhaling infections aerosols from milk, faeces, urina, and birth products from infected ruminants. This study used a 2-year longitudinal approach to investigate C. ...


Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, centr...

Pires, Humberto; Santos-Silva, Sérgio; Cruz, Andreia V.S.; Cardoso, Luís; Lopes, Ana Patrícia; Pereira, Maria; Nóbrega, Carmen; Mega, Cristina

Coxiella burnetii is the etiologic agent of Q fever, a worldwide zoonosis. Cattle, sheep and goats are considered the main reservoirs of the disease. Transmission to humans occurs mainly through the inhalation of infectious aerosols from milk, faeces, urine, and birth products from infected ruminants. In this study, a 2-year longitudinal approach was performed to ascertain the excretion of C. burnetii in bulk t...


Hunting for answers: Assessing Brucella spp. seroprevalence and risks in red de...

Pires, Humberto; Cardoso, Luís; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Silva, Sérgio Santos; Matos, Manuela; Pintado, Cristina Santos

Between 2016 and 2023, a cross-sectional study was conducted in the central region of Portugal in order to better understand the epidemiology and public health risks resulting from the handling and consumption of game animals infected with Brucella spp. The seroprevalence and risk factors for Brucella spp. seropositivity were evaluated. Antibodies against Brucella spp. were determined using a commercial enzyme-...


Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, centr...

Pires, Humberto; Silva, Sérgio Santos; Cruz, Andreia V.S.; Cardoso, Luís; Lopes, Ana Patrícia; Pereira, Maria A.; Nóbrega, Carmen; Mega, Ana Cristina

Coxiella burnetii is the etiologic agent of Q fever, a worldwide zoonosis. Cattle, sheep and goats are considered the main reservoirs of the disease. Transmission to humans occurs mainly through the inhalation of infectious aerosols from milk, faeces, urine, and birth products from infected ruminants. In this study, a 2-year longitudinal approach was performed to ascertain the excretion of C. burnetii in bulk t...


Brucella spp. seroprevalence in wild ungulates in Portugal

Pires, Humberto; Matos, Manuela; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Santos Pintado, Cristina Maria Baptista

Brucella spp. seroprevalence in wild ungulates in Portugal.


Seroprevalence of West Nile Virus in vultures in the Centre region of Portugal

Loureiro, Filipa; Sargo, R.; Sousa, L.; Silva D.; Silva, F.; Lopes, F.; Peñuela, R.; Azorín, B.; Pires, Humberto; Cardoso, Luis; Lopes, Ana Patrícia

West Nile Virus (WNV) is a vector-borne pathogen with zoonotic potential widely spread throughout the world. The transmission cycle is maintained by mosquitoes (mainly Culex spp.) and wild birds. Human, equines and other mammals are incidental hosts, whichare unable to amplify the agent in their blood circulation. Two lineages has been identified as disease-causing in humans and other animals: WNV-1 and WNV-2, ...


Seroprevalence of Hepatitis E Virus in wild boar (Sus scrofa) and in red deer (...

Pires, Humberto; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Matos, Manuela; Pintado, Cristina; Figueira, Luis Manuel Faria

Hepatitis E virus (HEV) is recognized as a zoonotic agent causing an emerging human viral disease in industrialized nations. HEV is primarily found in domestic pigs and wild boars, which serve as the primary animal host for the virus globally, but also camels and deers, which serve as reservoirs. In order to investigate the importance of the wild red deer (Cervus elaphus) and wild boar (Sus scrofa) in the epide...


A serological survey of Brucella spp. in red deer (Cervus elaphus) in Portugal

Pires, Humberto; Matos, Manuela; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Santos Pintado, Cristina Maria Baptista

Wildlife has been identified as important sentinels for the surveillance of zoonotic pathogens such Brucella infection. Systematic brucellosis monitoring in wildlife is not demanded by regulatory acts, but several studies have reported the presence of this infection in European countries. In order to investigate the importance of red deer (Cervus elaphus) in the epidemiology of Brucella infection in Portugal, a...


A preliminar study on Brucella spp. seroprevalence in wild boar (Sus scrofa) in...

Pires, Humberto; Matos, Manuela; Cardoso, Luis; Lopes, Ana Patrícia; Fontes, Maria da Conceição; Pintado, Cristina

Various species of the genus Brucella are highly virulent zoonotic agents. Brucella melitensis, B. abortus, and B. suis area broadly spread worldwide and animal brucellosis has a significant economic impact. This zoonotic disease affects both domestic pigs and wild pigs such as wild boar (Sus scrofa) In order to investigate the importance of wild boar (Sus scrofa) in the epidemiology of Brucella infection in Po...


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