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Genetic Diversity of Salmonella enterica subsp. enterica Serovar Enteritidis fr...

Leão, Célia; Silveira, Leonor; Usié, Ana; Gião, Joana; Clemente, Lurdes; Themudo, Patricia; Amaro, Ana; Pista, Angela

Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) is one of the leading causes of foodborne infections associated with broilers and laying hens. Portugal has had the lowest notification rates of salmonellosis in recent years, due to the vaccinations of layer and breeder flocks and strict compliance with biosecurity measures. However, data about the genetic diversity of S. Enteritidis in P...


Aumento de casos de Salmonella Typhimurium em 2024: caracterização fenotípica e...

Tomás, Alexandra; Henriques, Ana Margarida; Caeiro, Raquel; Pista, Angela; Silveira, Leonor

Salmonelose é a segunda zoonose mais frequente a nível mundial. É causada por Salmonella enterica, sendo S. Typhimurium um dos serotipos mais reportados na União Europeia. Um dos maiores riscos para a saúde pública é a presença de Salmonella spp. em alimentos, e consequente associação a surtos que podem atingir uma dimensão transfronteiriça. Este estudo teve como principal objetivo caraterizar fenotípica e geno...


Caracterização genómica de Salmonella Newport: identificação de clusters genéti...

Caeiro, Raquel; Tomás, Alexandra; Henriques, Ana Margarida; Pista, Angela; Silveira, Leonor

Salmonella enterica subsp. enterica serovar Newport (S. Newport) é um serotipo pouco frequente na União Europeia. No entanto, devido à sua associação com alguns surtos de origem alimentar, a sua importância não deve ser negligenciada. Em Portugal, os dados sobre a infeção por S. Newport são escassos. Este estudo teve como principais objetivos caracterizar fenotípica e genotipicamente isolados clínicos de S. New...


Raw milk cheeses from Beira Baixa, Portugal - A contributive study for the micr...

Mendonça, Rita; Furtado, Rosália; Coelho, Anabela; Correia, Cristina Belo; Suyarko, Elena; Borges, Vítor; Gomes, João Paulo; Pista, Angela

Due to specific bacterial microbiota, raw milk cheeses have appreciated sensory properties. However, they may pose a threat to consumer safety due to potential pathogens presence. This study evaluated the microbiological contamination of 98 raw milk cheeses from Beira Baixa, Portugal. Presence and enumeration of Coagulase Positive Staphylococci (CPS), Listeria monocytogenes, Salmonella spp., pathogenic Escheric...


Caracterização fenotípica e genotípica de isolados de Salmonella Typhi e Paraty...

Henriques, Ana Margarida; Caeiro, Raquel; Tomás, Alexandra; Silveira, Leonor; Pista, Angela

Os serotipos Typhi e Paratyphi causam infeções sistémicas graves, sendo crucial a administração de antibioterapia adequada. No entanto, o aumento crescente da taxa de multirresistência aos antibióticos, especialmente no caso de Salmonella Typhi, dificulta a escolha dos antibióticos a administrar. Em Portugal, os casos de febre tifoide e paratifoide são raros. Com este estudo pretendeu-se identificar e caracteri...


Building an International One Health Strain Level Database to Characterise the ...

Perestrelo, Sara; Amaro, Ana; Brouwer, Michael S.M.; Clemente, Lurdes; Ribeiro Duarte, Ana Sofia; Kaesbohrer, Annemarie; Karpíšková, Renata

Antimicrobial resistance (AMR) is one of the top public health threats nowadays. Among the most important AMR pathogens, Escherichia coli resistant to extended spectrum cephalosporins (ESC-EC) is a perfect example of the One Health problem due to its global distribution in animal, human, and environmental sources and its resistant phenotype, derived from the carriage of plasmid-borne extended-spectrum and AmpC ...


Occurrence of Multidrug-Resistant Bacteria Resulting from the Selective Pressur...

Rodrigues, Inês C.; Ribeiro-Almeida, Marisa; Ribeiro, Jorge; Silveira, Leonor; Prata, Joana C.; Pista, Angela; Martins da Costa, Paulo

Because of public health concerns, much greater scrutiny is now placed on antibiotic use in pets, especially for antimicrobial agents that have human analogs. Therefore, this study aimed to characterize the phenotypic and genotypic profiles of multidrug-resistant bacteria isolated from nasal swabs samples taken from a one-year-old male Serra da Estrela dog with rhinorrhea that was treated with amikacin. An exte...


Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci i...

Praça, Joana; Furtado, Rosália; Coelho, Anabela; Correia, Cristina Belo; Borges, Vítor; Gomes, João Paulo; Pista, Angela; Batista, Rita

Traditional cheeses are part of the Portuguese gastronomic identity, and raw milk of autochthonous species is a common primary ingredient. Here, we investigated the presence of Listeria monocytogenes, Coagulase Positive Staphylococci (CPS) and pathogenic Escherichia coli, as well as of indicator microorganisms (E. coli and other Listeria spp.) in 96 cured raw milk cheeses from the Alentejo region. Whole genome ...


Are Food Producing animals a source of multidrug-resistant E. coli and Salmonel...

Pista, Angela; Silveira, Leonor; Gonçalves, Carlota; Costa, Sara; Carolino, Catarina; Santo, Bianca; Rosa, Rute; Penteado, Margarida; Alves, Margarida

Objectives: To assess the role of food-producing animals as potential transmission vehicles of Salmonella spp. and E. coli to Humans, in order to understand the epidemiology and population structure of these zoonotic agents in Portugal.


Multidrug-resistant Escherichia coli and Salmonella spp. in pigs slaughtered fo...

Pista, Angela; Silveira, Leonor; Costa, Sara; Rosa, Rute; Gonçalves, Carlota; Alves, Margarida; Belas, Adriana; Santos, Isabel; Lima, Ana

Objective: To assess the role of pig reservoir as potential transmission vehicle of Salmonella spp. and E. coli to humans, in order to understand the epidemiology and population structure of these zoonotic agents in Portugal.


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