6 documents found, page 1 of 1
Cellular reprogramming as a tool to model human aging in a dish
Pitrez, Patrícia R.; Monteiro, Luís M.; Borgogno, Oliver; Nissan, Xavier; Mertens, Jerome; Ferreira, LinoThe design of human model systems is highly relevant to unveil the underlying mechanisms of aging and to provide insights on potential interventions to extend human health and life span. In this perspective, we explore the potential of 2D or 3D culture models comprising human induced pluripotent stem cells and transdifferentiated cells obtained from aged or age-related disorder-affected donors to enhance our un...
Vulnerability of progeroid smooth muscle cells to biomechanical forces is media...
Pitrez, Patrícia R.; Estronca, Luís Miguel Beicinha Branco; Monteiro, Luís Miguel Marques; Colell, Guillem; Vazão, HelenaHutchinson-Gilford Progeria Syndrome (HGPS) is a premature aging disease in children that leads to early death. Smooth muscle cells (SMCs) are the most affected cells in HGPS individuals, although the reason for such vulnerability remains poorly understood. In this work, we develop a microfluidic chip formed by HGPS-SMCs generated from induced pluripotent stem cells (iPSCs), to study their vulnerability to flow...
Substrate Topography Modulates Cell Aging on a Progeria Cell Model
Pitrez, Patrícia R.; Estronca, Luís Miguel Beicinha Branco; Vazão, Helena; Egesipe, Anne-Laure; Le Corf, Amélie; Navarro, Claire; Lévy, NicolasAging is characterized by a progressive accumulation of cellular damage, which leads to impaired function. Little is known whether substrates can influence cell aging. This is of utmost importance in the development of medical devices that are in contact with human tissue for long periods of time. To address this question, we have used an accelerated aging cell model derived from Hutchinson-Gilford Progeria Syn...
Physiological and Pathological Vascular Aging
Pitrez, Patrícia R.; Aires, Helena R.; Tomé, Inês; Ferreira, Rita Sá; Ferreira, LinoAging is a risk factor for cardiovascular diseases. Through aging, blood vessels become stiffer, less elastic and, thus, with less ability to contract. The objectives of this chapter are to review (i) recent progresses in the characterization of physiological and pathological vascular aging and (ii) in vitro platforms to study vascular aging. Initially, we will discuss the causes and biomarkers of vascular agin...
A High Throughput Phenotypic Screening reveals compounds that counteract premat...
Lo Cicero, Alessandra; Jaskowiak, Anne-Laure; Egesipe, Anne-Laure; Tournois, Johana; Brinon, Benjamin; Pitrez, Patrícia R.; Ferreira, LinoHutchinson-Gilford progeria syndrome (HGPS) is a rare fatal genetic disorder that causes systemic accelerated aging in children. Thanks to the pluripotency and self-renewal properties of induced pluripotent stem cells (iPSC), HGPS iPSC-based modeling opens up the possibility of access to different relevant cell types for pharmacological approaches. In this study, 2800 small molecules were explored using high-th...
Influence of the pre-treatments on the properties of biodegradable films from b...
Costa, Joana F.; Pitrez, Patrícia R.; Rocha, Cristina M. R.; Freitas, Olga M.; Crispim, Alfredo; Delerue-Matos, Cristina; Gonçalves, Maria PilarThe replacement of conventional synthetic films and coatings by biodegradable alternatives reduces the use of non-renewable resources and waste disposal problems. Considering that Portugal is a major producer of leather, and consequently a large producer of related wastes, in this research, bovine hair was tested for the production of biodegradable films directly by thermo-compression, allowing waste valorisati...