22 documents found, page 1 of 3
Highly elastic and bioactive bone biomimetic scaffolds based on platelet lysate...
Ribeiro, J. P.; Domingues, R. M. A.; Babo, P. S.; Nogueira, L.; Reseland, J.; Reis, R. L.; Gomez-Florit, Manuel; Gomes, Manuela E.Bone is a vascularized organic-inorganic composite tissue that shows a heavily-mineralized extracellular matrix (ECM) on the nanoscale. Herein, the nucleation of calcium phosphates during the biomineralization process was mimicked using negatively-charged cellulose nanocrystals (CNCs). These mineralized-CNCs were combined with platelet lysate to produce nanocomposite scaffolds through cryogelation to mimic bone...
Magnetically-assisted 3D bioprinting of anisotropic tissue-mimetic constructs
Pardo, Alberto; Bakht, Syeda Mahwish; Gomez-Florit, Manuel; Rial, Ramón; Monteiro, Rosa Conceiçao Freitas; Teixeira, Simão P. B.; Taboada, PabloRecreating the extracellular matrix organization and cellular patterns of aniso-tropic tissues in bioengineered constructs remains a significant biofabrication challenge. Magnetically-assisted 3D bioprinting strategies can be exploited to fabricate biomimetic scaffolding systems, but they fail to provide control over the distribution of magnetic materials incorporated in the bioinks while pre-serving the fideli...
Xeno-free bioengineered human skeletal muscle tissue using human platelet lysat...
Fernández-Garibay, X.; Gomez-Florit, Manuel; Domingues, Rui Miguel Andrade; Gomes, Manuela E.; Fernández-Costa, J. M.; Ramón-Azcón, J.Bioengineered human skeletal muscle tissues have emerged in the last years as new in vitro systems for disease modeling. These bioartificial muscles are classically fabricated by encapsulating human myogenic precursor cells in a hydrogel scaffold that resembles the extracellular matrix. However, most of these hydrogels are derived from xenogenic sources, and the culture media is supplemented with animal serum...
Controlling the fate of regenerative cells with engineered platelet-derived ext...
Graça, Ana L.; Gomez-Florit, Manuel; Osório, Hugo; Rodrigues, Márcia T.; Domingues, Rui Miguel Andrade; Reis, R. L.; Gomes, Manuela E.Extracellular vesicles (EVs) have emerged as cell-free nanotherapeutic agents for the potential treatment of multiple diseases and for tissue engineering and regenerative medicine strategies. Nevertheless, the field has typically relied on EVs derived from stem cells, the production of which in high quantities and high reproducibility is still under debate. Platelet-derived EVs were produced by a freeze–thaw me...
Bioengineered 3D living fibers as in vitro human tissue models of tendon physio...
Calejo, Isabel; Labrador-Rached, Claudia J.; Gomez-Florit, Manuel; Docheva, Denitsa; Reis, R. L.; Domingues, Rui Miguel Andrade; Gomes, Manuela E.Clinically relevant in vitro models of human tissue's health and disease are urgently needed for a better understanding of biological mechanisms essential for the development of novel therapies. Herein, physiological (healthy) and pathological (disease) tendon states are bioengineered by coupling the biological signaling of platelet lysate components with controlled 3D architectures of electrospun microfibers t...
Magnetic nanocomposite hydrogels for tissue engineering: design concepts and re...
Pardo, Alberto; Gomez-Florit, Manuel; Barbosa, Silvia; Taboada, Pablo; Domingues, Rui Miguel Andrade; Gomes, Manuela E.Most tissues of the human body are characterized by highly anisotropic physical properties and biological organization. Hydrogels have been proposed as scaffolding materials to construct artificial tissues due to their water-rich composition, biocompatibility and tunable properties. However, unmodified hydrogels are typically composed of randomly oriented polymer networks, resulting in homogeneous structures wi...
Multifunctional surfaces for improving soft tissue integration
Vilaça, A.; Domingues, Rui Miguel Andrade; Tiainen, H.; Mendes, B. B.; Barrantes, A.; Reis, R. L.; Gomes, Manuela E.; Gomez-Florit, ManuelMetallic implants are widely used in diverse clinical applications to aid in recovery from lesions or to replace native hard tissues. However, the lack of integration of metallic surfaces with soft tissue interfaces causes the occurrence of biomaterial-associated infections, which can trigger a complicated inflammatory response and, ultimately, implant failure. Here, a multifunctional implant surface showing na...
Human platelet lysate-loaded Poly(ethylene glycol) hydrogels induce stem cell c...
Chahal, A. S.; Gomez-Florit, Manuel; Domingues, Rui Miguel Andrade; Gomes, Manuela E.; Tiainen, H.Platelet lysates (PL) contain a selection of proteins and growth factors (GFs) that are known to mediate cell activity. Many of these biomolecules have been identified as chemoattractants with the capacity to induce cell migration. In order to effectively deliver and retain these biomolecules to the site of injury, a scaffold containing PL could be an option. We use poly(ethylene glycol) (PEG) hydrogels consist...
Decellularized kidney extracellular matrix bioinks recapitulate renal 3D microe...
Sobreiro-Almeida, Rita; Gomez-Florit, Manuel; Quinteira, Rita; Reis, R. L.; Gomes, Manuela E.; Neves, N. M.Decellularized extracellular matrices (ECMs) are able to provide the necessary and specific cues for remodeling and maturation of tissue-specific cells. Nevertheless, their use for typical biofabrication applications requires chemical modification or mixing with other polymers, mainly due to the limited viscoelastic properties. In this study, we hypothesize that a bioink exclusively based on decellularized kidn...
Engineering next-generation bioinks with nanoparticles: moving from reinforceme...
Bakht, S. M.; Pardo, A.; Gomez-Florit, Manuel; Reis, R. L.; Domingues, R. M. A.; Gomes, Manuela E.The application of additive manufacturing in the biomedical field has become a hot topic in the last decade owing to its potential to provide personalized solutions for patients. Different bioinks have been designed trying to obtain a unique concoction that addresses all the needs for tissue engineering and drug delivery purposes, among others. Despite the remarkable progress made, the development of suitable b...