29 documents found, page 1 of 3
Comparative proteomics of Geobacter sulfurreducens PCAT in response to acetate,...
Mollaei, Monir; Timmers, Peer H. A.; Suarez-Diez, Maria; Boeren, Sjef; van Gelder, Antonie H.; Stams, Alfons Johannes Maria; Plugge, Caroline M.Geobacter sulfurreducens is a model bacterium to study the degradation of organic compounds coupled to the reduction of Fe(III). The response of G. sulfurreducens to the electron donors acetate, formate, hydrogen and a mixture of all three with Fe(III) citrate as electron acceptor was studied using comparative physiological and proteomic approaches. Variations in the supplied electron donors resulted in differe...
Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MP...
Mollaei, Monir; Suarez-Diez, Maria; Sedano-Nunez, Vicente T.; Boeren, Sjef; Stams, Alfons Johannes Maria; Plugge, Caroline M.We established a syntrophic coculture of Syntrophobacter fumaroxidans MPOB<sup>T</sup> (SF) and Geobacter sulfurreducens PCA<sup>T</sup> (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron a...
Effect of sulfate on carbon monoxide conversion by a thermophilic syngas-fermen...
Alves, J. I.; Visser, Michael; Arantes, Ana Luísa; Nijsse, Bart; Plugge, Caroline M.; Alves, M. M.; Stams, Alfons Johannes MariaA syngas-degrading enrichment culture, culture T-Syn, was dominated by a bacterium closely related to Desulfofundulus australicus strain AB33T (98% 16S rRNA gene sequence identity). Culture T-Syn could convert high CO concentrations (from pCO 34 kPa to pCO 170 kPa), both in the absence and in the presence of sulfate as external electron acceptor. The products formed from CO conversion were H2 and acetate. With ...
Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic aceta...
Timmers, Peer H. A.; Vavourakis, Charlotte D.; Kleerebezem, Robbert; Damste, Jaap S. Sinninghe; Muyzer, Gerard; Stams, Alfons Johannes MariaAnaerobic syntrophic acetate oxidation (SAO) is a thermodynamically unfavorable process involving a syntrophic acetate oxidizing bacterium (SAOB) that forms interspecies electron carriers (IECs). These IECs are consumed by syntrophic partners, typically hydrogenotrophic methanogenic archaea or sulfate reducing bacteria. In this work, the metabolism and occurrence of SAOB at extremely haloalkaline conditions wer...
Comparative proteome analysis of propionate degradation by Syntrophobacter fuma...
Sedano-Núñez, Vicente T.; Boeren, Sjef; Stams, Alfons Johannes Maria; Plugge, Caroline M.Syntrophobacter fumaroxidans is a sulfatereducing bacterium able to grow on propionate axenically or in syntrophic interaction with methanogens or other sulfatereducing bacteria. We performed a proteome analysis of S. fumaroxidans growing with propionate axenically with sulfate or fumarate, and in syntrophy with Methanospirillum hungatei, Methanobacterium formicicum or Desulfovibrio desulfuricans. Special atten...
Organic acid production from potato starch waste fermentation by rumen microbia...
Palakawong Na Ayudthaya, Susakul; van de Weijer, Antonius H. P.; van Gelder, Antonie H.; Stams, Alfons Johannes Maria; de Vos, Willem M.Background: Exploring different microbial sources for biotechnological production of organic acids is important. Dutch and Thai cow rumen samples were used as inocula to produce organic acid from starch waste in anaerobic reactors. Organic acid production profiles were determined and microbial communities were compared using 16S ribosomal ribonucleic acid gene amplicon pyrosequencing. Results In both reactors, ...
Sodium lauryl ether sulfate (SLES) degradation by nitrate-reducing bacteria
Paulo, Ana M. S.; Aydin, Rozelin; Dimitrov, Mauricio R.; Vreeling, Harm; Cavaleiro, Ana Júlia; García-Encina, Pedro A.; Stams, Alfons Johannes MariaThe surfactant sodium lauryl ether sulfate (SLES) is widely used in the composition of detergents and frequently ends up in wastewater treatment plants (WWTPs). While aerobic SLES degradation is well studied, little is known about the fate of this compound in anoxic environments, such as denitrification tanks of WWTPs, nor about the bacteria involved in the anoxic biodegradation. Here, we used SLES as sole carb...
Evaluation and optimization of PCR primers for selective and quantitative detec...
Timmers, Peer H. A.; Widjaja-Greefkes, H. C. Aura; Plugge, Caroline M.; Stams, Alfons Johannes MariaSince the discovery that anaerobic methanotrophic archaea (ANME) are involved in the anaerobic oxidation of methane coupled to sulfate reduction in marine sediments, different primers and probes specifically targeting the 16S rRNA gene of these archaea have been developed. Microbial investigation of the different ANME subtypes (ANME-1; ANME-2a, b, and c; and ANME-3) was mainly done in sediments where specific s...
Thiosulfate conversion to sulfide by a haloalkaliphilic microbial community in ...
Sousa, João A. B.; Bijmans, Martijn F. M.; Stams, Alfons Johannes Maria; Plugge, Caroline M.In industrial gas biodesulfurization systems, where haloalkaline conditions prevail, a thiosulfate containing bleed stream is produced. This bleed stream can be treated in a separate bioreactor by reducing thiosulfate to sulfide and recycling it. By performing treatment and recycling of the bleed stream, its disposal decreases and less caustics are required to maintain the high pH. In this study, anaerobic micr...
Syntrophic acetate oxidation at haloalkaline conditions
Timmers, Peer H. A.; Vavourakis, C.; Muyzer, G.; Sorokin, D.; Stams, Alfons Johannes Maria; Plugge, Caroline M.Syntrophic acetate oxidation (SAO) is a process where syntrophic acetate-oxidizing bacteria (SAOB) convert acetate to form CO2 and reducing equivalents. These reducing equivalents are utilized by a syntrophic partner which in most cases is a hydrogenotrophic methanogen or sulfate-reducing bacterium. Until now, SAO has been found to be dominant over aceticlastic methanogenesis under extreme conditions such as hi...