12 documents found, page 1 of 2
Sociodemographic, Clinical, and Behavioral Factors Associated With Sexual Trans...
Miranda, M; Pimentel, V; Graça, J; Seabra, S; Sebastião, C; Diniz, A; Faria, D; Teófilo, E; Roxo, F; Maltez, F; Germano, I; Oliveira, J; Ferreira, JIntroduction: Sexually transmitted infections (STIs) continue to occur at high levels. According to the WHO, each year there are an estimated 374 million new infections with syphilis, gonorrhea, chlamydia, and trichomoniasis. STIs are associated with an increased risk of acquiring HIV infection. Migrants are reportedly highly affected by STIs. Objectives: This study aims to characterize factors associated with ...
HIV-1-Transmitted Drug Resistance and Transmission Clusters in Newly Diagnosed ...
Pingarilho, M; Pimentel, V; Miranda, M; Silva, AR; Diniz, A; Ascenção, B; Piñeiro, C; Koch, C; Rodrigues, C; Caldas, C; Morais, C; Faria, DObjective: To describe and analyze transmitted drug resistance (TDR) between 2014 and 2019 in newly infected patients with HIV-1 in Portugal and to characterize its transmission networks. Methods: Clinical, socioepidemiological, and risk behavior data were collected from 820 newly diagnosed patients in Portugal between September 2014 and December 2019. The sequences obtained from drug resistance testing were us...
Implementing an Influenza Vaccine Effectiveness Study in a Hospital Context in ...
Machado, A; Gomez, V; Panarra, A; Poças, J; Corte-Real, R; Peres, MJ; Nunes, BIntroduction: The project ‘Integrated Monitoring of Vaccines in Europe’ aimed to measure seasonal influenza vaccine effectiveness against hospitalised adults, aged 65 years and over, with influenza. We describe the protocol implementation in Portugal. Material and Methods: We implemented a test-negative design, targeting community-dwelling patients aged 65 years old and over hospitalised with severe acute respi...
Heparan sulfate glycosaminoglycans: (un)expected allies in cancer clinical mana...
Faria-Ramos, I; Poças, J; Marques, C; Santos-Antunes, J; Macedo, G; Reis, CA; Magalhães, AIn an era when cancer glycobiology research is exponentially growing, we are witnessing a progressive translation of the major scientific findings to the clinical practice with the overarching aim of improving cancer patients’ management. Many mechanistic cell biology studies have demonstrated that heparan sulfate (HS) glycosaminoglycans are key molecules responsible for several molecular and biochemical proces...
Transcriptome Reprogramming of CD11b(+) Bone Marrow Cells by Pancreatic Cancer ...
Maia, J; Otake, AH; Poças, J; Carvalho, AS; Beck, HC; Magalhães, A; Matthiesen, R; Strano Moraes, MC; Costa-Silva, BPancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation of CD11b+ bone marrow (BM) cells, which support PC liver metastasis. We here show that PC-EVs also bind to CD11b+ BM c...
Different isolation approaches lead to diverse glycosylated extracellular vesic...
Freitas, D; Balmaña, M; Poças, J; Campos, D; Osório, H; Konstantinidi, A; Vakhrushev, SY; Magalhães, A; Reis, CAExtracellular vesicles (EVs) are a heterogeneous group of small secreted particles involved in inter-cellular communication and mediating a broad spectrum of biological functions. EVs cargo iscomposed of a large repertoire of molecules, including glycoconjugates. Herein, we report the firststudy on the impact of the isolation strategy on the EV populations’glycosylation profile. The use ofdifferent state-of-the...
HIV-2 infection is associated with preserved GALT homeostasis and epithelial in...
Fernandes, S; Pires, A; Matoso, P; Ferreira, C; Nunes-Cabaço, H; Correia, L; Valadas, E; Poças, J; Pacheco, P, et al.The mechanisms that enable preservation of gut mucosal integrity during persistent viral replication and inherent inflammation remain unclear. Here, we investigated, for the first time, gut homeostasis in HIV-2 infection, a naturally occurring form of attenuated HIV disease. We found viral replication in both sigmoid and ileum of asymptomatic HIV-2+ patients (range: 240-851 circulating CD4+T-cells per μl) despi...
Glycosylation in cancer: Selected roles in tumour progression, immune modulatio...
Rodrigues, JG; Balmaña, M; Macedo, JA; Poças, J; Fernandes, Â; Freitas-Junior, JCM; Pinho, SS; Gomes, J; Magalhães, A; Gomes, C; Mereiter, S; Reis, CATumour metastasis is the main cause of cancer related deaths. Metastasis is an intricate multi-step process that requires the acquisition of several cancer cell features, including the modulation of tumour cell migration, adhesion, invasion, and immune evasion. Changes in the cellular glycosylation are associated with malignant transformation of cancer cells, tumour progression and ultimately, metastasis format...
HIV-2 Integrase Polymorphisms and Longitudinal Genotypic Analysis of HIV-2 Infe...
Cavaco-Silva, J; Abecasis, A; Miranda, AC; Poças, J; Narciso, J; Águas, MJ; Maltez, F; Almeida, I; Germano, I; Diniz, A; Gonçalves, MF; Gomes, PTo characterize the HIV-2 integrase gene polymorphisms and the pathways to resistance of HIV-2 patients failing a raltegravir-containing regimen, we studied 63 integrase strand transfer inhibitors (INSTI)-naïve patients, and 10 heavily pretreated patients exhibiting virological failure while receiving a salvage raltegravir-containing regimen. All patients were infected by HIV-2 group A. 61.4% of the integrase r...
Host- and microbe-related risk factors for and pathophysiology of fatal Rickett...
Sousa, R; França, A; Nóbrega, SD; Belo, A; Amaro, M; Abreu, T; Poças, J; Proença, P; Vaz, J; Torgal, J; Bacellar, F; Ismail, N; Walker, DBACKGROUND: The pathophysiologic mechanisms that determine the severity of Mediterranean spotted fever (MSF) and the host-related and microbe-related risk factors for a fatal outcome are incompletely understood. METHODS: This prospective study used univariate and multivariate analyses to determine the risk factors for a fatal outcome for 140 patients with Rickettsia conorii infection admitted to 13 Portuguese h...