8 documents found, page 1 of 1
Squamous cell carcinoma with sarcomatous transformation of the penis
Santos, J; Cabrebra, R; Neves, B; Silva, E; Polónia, AMalignant tumors of the penis are rare, most of them being squamous cell carcinomas (SCCs). We report the case of a 75-year-old man with a large penile mass submitted to partial penectomy. The specimen showed an exophytic mass involving the glans, coronal sulcus, and prepuce. Microscopic examination showed a carcinoma with two distinct areas: a mixed SCC and a sarcomatoid carcinoma. The SCC component had areas ...
Digital pathology workflow implementation at ipatimup
Eloy, C; Vale, J; Curado, M; Polónia, A; Campelos, S; Caramelo, A; Sousa, R; Sobrinho-Simões, MThe advantages of the digital methodology are well known. In this paper, we provide a detailed description of the process for the digital transformation of the pathology laboratory at IPATIMUP, the major modifications that operate throughout the processing pipeline, and the advantages of its implementation. The model of digital workflow implementation at IPATIMUP demonstrates that careful planning and adoption ...
Carcinoembryonic antigen carrying SLe X as a new biomarker of more aggressive g...
Gomes, C; Almeida, A; Barreira, A; Calheiros, J; Pinto, F; Abrantes, R; Costa, A; Polónia, A; Campos, D; Osorio, H; Sousa, HS; Pinto-De-Sousa, JMalignant transformation of gastric cells is accompanied by the deregulated expression of glycosyltransferases leading to the biosynthesis of tumor-associated glycans such as the sialyl-Lewis X antigen (SLex). SLex presence on cell surface glycoconjugates increases the invasive capacity of gastric cancer cells and is associated with tumor metastasis. ST3Gal IV enzyme is involved in the synthesis of SLex antigen...
Detection of HER2 from Haematoxylin-Eosin slides through a cascade of deep lear...
Barbera, DL; Polónia, A; Roitero, K; Conde-Sousa, E; Mea, VDBreast cancer is the most frequently diagnosed cancer in woman. The correct identification of the HER2 receptor is a matter of major importance when dealing with breast cancer: an over-expression of HER2 is associated with aggressive clinical behaviour; moreover, HER2 targeted therapy results in a significant improvement in the overall survival rate. In this work, we employ a pipeline based on a cascade of deep...
O-glycans truncation modulates gastric cancer cell signaling and transcription ...
Freitas, D; Campos, D; Gomes, J; Pinto, F; Macedo, JA; Matos, R; Mereiter, S; Pinto, MT; Polónia, A; Gärtner, F; Magalhães, A; Reis, CABackground: Changes in glycosylation are known to play critical roles during gastric carcinogenesis. Expression of truncated O-glycans, such as the Sialyl-Tn (STn) antigen, is a common feature shared by many cancers and is associated with cancer aggressiveness and poor-prognosis. Methods: Glycoengineered cell lines were used to evaluate the impact of truncated O-glycans in cancer cell biology using in vitro fun...
SRC inhibition prevents P-cadherin mediated signaling and function in basal-lik...
Ribeiro, AS; Nobre, AR; Mendes, N; Almeida, J; Vieira, AF; Sousa, B; Carvalho, F; Monteiro, J; Polónia, A; Fonseca, M; Sanches, J; Santos, N; Seruca, RBACKGROUND: Basal-like breast cancer (BLBC) is a poor prognosis subgroup of triple-negative carcinomas that still lack specific target therapies and accurate biomarkers for treatment selection. P-cadherin is frequently overexpressed in these tumors, promoting cell invasion, stem cell activity and tumorigenesis by the activation of Src-Family kinase (SRC) signaling. Therefore, our aim was to evaluate if the trea...
Counting invasive breast cancer cells in the HER2 silver in-situ hybridization ...
Polónia, A; Eloy, C; Pinto, J; Braga, A; Oliveira, G; Schmitt, FAIM: To evaluate the intraobserver and interobserver reproducibility of the HER2 in-situ hybridization (ISH) test in breast cancer by measuring the impact of counting different numbers of invasive cancer cells. METHODS AND RESULTS: A cohort of 101 primary invasive breast cancer cases were evaluated for HER2 gene amplification by silver ISH, and the concordance among four observers with different levels of exper...
Epitope mapping of a new anti-Tn antibody detecting gastric cancer cells.
Persson, N; Stuhr-Hansen, N; Risinger, C; Mereiter, S; Polónia, A; Polom, K; Kovács, A; Roviello, F; Reis, CA; Welinder, C; Danielsson, L; Jansson, BHere, we introduce a novel scFv antibody, G2-D11, specific for two adjacent Tn-antigens (GalNAc- Ser/Thr) binding equally to three dimeric forms of the epitope, Ser-Thr, Thr-Thr and Thr-Ser. Compared to other anti-Tn reagents, the binding of G2-D11 is minimally influenced by the peptide structure, which indicates a high degree of carbohydrate epitope dominance and a low influence from the protein backbone. With...