34 documents found, page 1 of 4

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Assessing process variable effects on laccase-mediated treatment for enhanced s...

Michelin, Michele; Gomes, Daniel Gonçalves; Freitas, Emanuelle N.; Alnoch, Robson C.; Salgado, José Carlos S.; Coelho, Eduardo João Louro

Lignocellulosic materials (LCM) have gained popularity as a substitute for petroleum, enabling the production of biofuels and various compounds. Eucalyptus bark residue (EBR), generated by pulp and paper mills, is a prime illustration of such materials. The conversion of holocellulose in these materials typically involves the application of enzymes. Nevertheless, these materials may still contain lignin, widely...


Pleurotus ostreatus and lentinus sajor-caju laccases for sulfamethoxazole biotr...

Sá, Helena; Michelin, Michele; Silvério, Sara Isabel Cruz; Polizeli, Maria de Lourdes T. M.; Silva, Ana Rita Pereira; Pereira, Luciana; Tavares, T.

Sulfamethoxazole (SMX) is an antibiotic commonly used to treat human and animal infections. Therefore, it is one of the most identified pharmaceuticals worldwide in surface water and wastewater treatment plants. This work exploited Pleurotus ostreatus and Lentinus sajor-caju to produce laccase by using agricultural and food residues as substrates, specifically vine pruning and brewer's spent grains. The fungal ...


Inhibition of enzyme hydrolysis of cellulose by phenols from hydrothermally pre...

Michelin, Michele; Ximenes, Eduardo; Polizeli, Maria de Lourdes T. M.; Ladisch, Michael R.

Relatively few studies have addressed the characterization of sugarcane straw (SCS) for production fermentable sugars through enzyme hydrolysis. Straw is a major co-product of the sugarcane harvest in Brazil that has potential to sustainably increase cellulosic feedstocks in Brazil by 50%. Pretreatment of 10% w/v straw with liquid hot water (LHW) at 180°C for 50min (severity, So, of 4.05), solubilizes hemicellu...


Assessing potential effects of a laccase extract over the enzymatichydrolysis o...

Gomes, Daniel Gonçalves; Michelin, Michele; Polizeli, Maria de Lourdes T. M.; Domingues, Lucília; Teixeira, J. A.

Lignocellulosic materials are rising as an alternative to petroleum, from which biofuels and numerous compounds may be produced. Eucalyptus barks, abundantly generated by pulp & paper mills, are a good example of such materials, being typically used for energy production. Holocellulose conversion of these materials is usually made by enzyme preparations, mainly acting on the hydrolysis of complex cellulose into...


Development of new eco-friendly supports for immobilization of enzymes based on...

De Andrades, Diandra; Robson Alnoch, Carlos; Vici, Ana Claudia; Sousa Alves, Gabrilela; Michelin, Michele; Silveira Buckeridge, Marcos

The pulp and paper industry generates a high volume of solid wastes that are usually burned to obtain energy, directed to landfills, or incinerated. Among the wastes generated in this process is the paper sludge, a residue rich in cellulose with low lignin content making it a useful raw material to produce high-value products such as cheap immobilization supports. In the current work, paper sludge was activated...


Immobilization and application of the recombinant xylanase GH10 of Malbranchea ...

Alnoch, Robson C.; Alves, Gabriela S.; Salgado, Jose C. S.; Andrades, Diandra de; Freitas, Emanuelle Neiverth de; Nogueira, Karoline M. V.; Vici, Ana C.

Xylooligosaccharides (XOS) are widely used in the food industry as prebiotic components. XOS with high purity are required for practical prebiotic function and other biological benefits, such as antioxidant and inflammatory properties. In this work, we immobilized the recombinant endo-1,4-β-xylanase of Malbranchea pulchella (MpXyn10) in various chemical supports and evaluated its potential to produce xylooligos...


Comparison in the Trichoderma longibrachiatum xyloglucanase production using ta...

Contato, Alex Graça; Vici, Ana Claudia; Aranha, Guilherme Mauro; Pinheiro, Vanessa Elisa; Oliveira, Tássio Brito de; Freitas, Emanuelle Neiverth de

in the control of the stretching and expansion of the plant cell wall. There are five types of enzymes known to be capable of cleaving the linear chain of xyloglucan, the most famous of them being the xyloglucanase (XEG). The immobilization can be used to solve problems related to stability, besides the economic benefits brought by the possibility of repeated use and recovery, decreasing the costs of production...


Immobilization and stabilization of acetyl xylan esteraseof Aspergillus nidulans

Alves, Gabriela S.; Andrades, Diandra de; Segato, Fernando; Michelin, Michele; Buckeridge, Marcos S.; Alnoch, Robson C.

[Excerpt] Acetyl xylan esterase (EC 3.1.1.72) are enzymes that cleave the ester bonds of the O-acetyl groups of the xylan backbone to create new sites for subsequent hydrolysis of other enzymes, such as endoxylanases (BIELY et al., 2014). D-xylose is one of the main constituents of xylan, the main class of hemicellulose. To degrade this structure, it is necessary that enzymatic hydrolysis occurs synergistically...


Nanocellulose production: exploring the enzymatic route and residues of pulp an...

Michelin, Michele; Gomes, Daniel Gonçalves; Romani, Aloia Perez; Polizeli, Maria de Lourdes T. M.; Teixeira, J. A.

Increasing environmental and sustainability concerns, caused by current population growth, has promoted a raising utilization of renewable bio-resources for the production of materials and energy. Recently, nanocellulose (NC) has been receiving great attention due to its many attractive features such as non-toxic nature, biocompatibility, and biodegradability, associated with its mechanical properties and those...


Sunflower stalk as a carbon source inductive for fungal xylanase production

de Souza, Jaqueline B.; Michelin, Michele; Amâncio, Francisco L. R.; Vital Brazil, Osiris A.; Polizeli, Maria de Lourdes T. M.; Ruzene, Denise S.

Enzymes represent a growing market due to their industrial applications, whose use on a large scale is restricted by their high cost of production. This stimulates studies with other sources such as the use of agro-industrial lignocellulosic residues in the medium composition for its production. Thus, in this work, in natura sunflower stalks (Helianthus annuus L.) and its respective alkaline and autohydrolysis ...


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