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Isolation and selection of Glyphosate herbicide tolerant fungi

Souza, Felipe Soares de; Souza, Hilton Marcelo de Lima; Oliveira , João Arthur dos Santos; Pamphile , João Alencar; Polonio, Julio Cesar

The objective of this work was to isolate fungi from an ecological reserve in the Brazilian Midwest and to select species tolerant to glyphosate, a herbicide widely used in agriculture. The purified isolates were subjected to qualitative tests to assess the oxidizing capacity of gallic acid and RBBR dye discoloration. Then, the selected fungi were identified through molecular biology techniques and used in herb...

Date: 2022   |   Origin: Oasisbr

BIOPROSPECÇÃO DE PEPTÍDEOS ANTIMICROBIANOS EM LARVAS DE CALLIPHORIDAE (DIPTERA)...

Caleffe, Ronaldo Roberto Tait; Oliveira, Stefany Rodrigues de; Polonio, Julio Cesar; Daquila, Bruno Vinicius; Ruvolo-Takasusuki, Maria Claudia Colla

Os insetos apresentam a maior biodiversidade entre a classe Animal e são fontes ricas de produtos biotecnológicos. Espécies da família Calliphoridae (Diptera) apresentam hábitos necrófagos durante a fase larval, assim são expostas a diferentes microrganismos, produzindo peptídeos antimicrobianos (AMPs) como sistema de defesa. O objetivo deste trabalho foi realizar um levantamento bibliográfico dos principais pe...

Date: 2021   |   Origin: Oasisbr

Bioprospection and molecular phylogeny of culturable endophytic fungi associate...

Oliveira, João Arthur dos Santos; Polli, Andressa Domingos; Polonio, Julio Cesar; Orlandelli, Ravely Casarotti; Conte, Hélio; Azevedo, João Lúcio

Endophytic microorganisms live inside the plants without causing any damage to their hosts. In the agricultural field, these endophytes might be a strategy of biological control for phytopathogens. We aimed to isolate endophytic fungi from yellow passion fruit (Passiflora edulis) leaves, evaluating its biocontrol capacity by in vitro antagonism against phytopathogen Colletotrichum sp. CNPU378. We also carried o...

Date: 2020   |   Origin: Oasisbr

Endoglucanase production by endophytic fungi isolated from Vitis labrusca L. wi...

Felber, Aretusa Cristina; Specian, Vânia; Orlandelli, Ravely Casarotti; Costa, Alessandra Tenório; Polonio, Julio Cesar; Mourão, Káthia Socorro Mathias

Endoglucanases are enzymes widely employed in different industrial fields, albeit with high production costs. Studies on new microbial sources and low-cost substrates are highly relevant, including those on agro-industrial. Current analysis evaluates peanut hull (PH) and sawdust (SD) as substrates for submerged cultures of 14 endophytic fungi isolated from grapevine (Vitis labrusca L.) cultivars Bordô and Conco...

Date: 2019   |   Origin: Oasisbr

Use of agro-industrial wastes as substrates for α-amylase production by endophy...

Orlandelli, Ravely Casarotti; Santos, Mariana Sanches; Polonio, Julio Cesar; Azevedo, João Lúcio; Pamphile, João Alencar

 Millions of tons of corn, pineapple, sugarcane and wheat are annually produced and their industrial processing generates large amounts of wastes. Current study evaluated the use of corncob (CC), pineapple peel (PP), sugarcane bagasse (SB) and wheat bran (WB) as substrates for α-amylase production by submerged cultures of nine endophytes from Piper hispidum Sw. Initially, fungi were grown on a starch medium and...

Date: 2017   |   Origin: Oasisbr

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