2 documents found, page 1 of 1
Fluorometric Liposome Screen for Inhibitors of a Physiologically Important Bact...
Fernandes, AS; Pombinho, AR; Teixeira-Duarte, CM; Morais-Cabral, JH; Harley, CAThe bacterial K+ homeostasis machinery is widely conserved across bacterial species, and different from that in animals. Dysfunction in components of the machinery has an impact on intracellular turgor, membrane potential, adaptation to changes in both extracellular pH and osmolarity, and in virulence. Using a fluorescence-based liposome flux assay, we have performed a high-throughput screen to identify novel i...
A SOX2 reporter system identifies gastric cancer stem-like cells sensitive to m...
Pádua, D; Barros, R; Amaral, AL; Mesquita, P; Freire, AF; Sousa, M; Maia, AF; Caiado, I; Fernandes, H; Pombinho, AR; Pereira, CF; Almeida, RGastric cancer remains a serious health burden with few therapeutic options. Therefore, the recognition of cancer stem cells (CSCs) as seeds of the tumorigenic process makes them a prime therapeutic target. Knowing that the transcription factors SOX2 and OCT4 promote stemness, our approach was to isolate stem-like cells in human gastric cancer cell lines using a traceable reporter system based on SOX2/OCT4 acti...