8 documents found, page 1 of 1
Stabilin-1 plays a protective role against Listeria monocytogenes infection thr...
Pombinho, R; Pinheiro, J; Resende, M; Meireles, D; Jalkanen, S; Sousa, S; Cabanes, DScavenger receptors are part of a complex surveillance system expressed by host cells to efficiently orchestrate innate immune response against bacterial infections. Stabilin-1 (STAB-1) is a scavenger receptor involved in cell trafficking, inflammation, and cancer; however, its role in infection remains to be elucidated. Listeria monocytogenes (Lm) is a major intracellular human food-borne pathogen causing seve...
Virulence gene repression promotes Listeria monocytogenes systemic infection
Pombinho, R; Vieira, A; Camejo, A; Archambaud, C; Cossart, P; Sousa, S; Cabanes, DThe capacity of bacterial pathogens to infect their hosts depends on the tight spatiotemporal regulation of virulence genes. The Listeria monocytogenes (Lm) metal efflux pump repressor CadC is highly expressed during late infection stages, modulating lipoprotein processing and host immune response. Here we investigate the potential of CadC as broad repressor of virulence genes. We show that CadC represses the e...
Listeria monocytogenes wall teichoic acid glycosylation promotes surface anchor...
Meireles, D; Pombinho, R; Carvalho, F; Sousa, S; Cabanes, DThe cell wall of Listeria monocytogenes (Lm), a major intracellular foodborne bacterial pathogen, comprises a thick peptidoglycan layer that serves as a scaffold for glycopolymers such as wall teichoic acids (WTAs). WTAs contain non-essential sugar substituents whose absence prevents bacteriophage binding and impacts antigenicity, sensitivity to antimicrobials, and virulence. Here, we demonstrated, for the firs...
Scavenger Receptors: Promiscuous Players during Microbial Pathogenesis
Pombinho, R; Sousa, S; Cabanes, DInnate immunity is the most broadly effective host defense, being essential to clear the majority of microbial infections. Scavenger Receptors comprise a family of sensors expressed in a multitude of host cells, whose dual role during microbial pathogenesis gained importance over recent years. SRs regulate the recruitment of immune cells and control both host inflammatory response and bacterial load. In turn, p...
MouR controls the expression of the Listeria monocytogenes Agr system and media...
Pinheiro, J; Lisboa, J; Pombinho, R; Carvalho, F; Carreaux, A; Brito, C; Pöntinen, A; Korkeala, H; Santos, NM; Morais-Cabral, JH; Sousa, S; Cabanes, DThe foodborne pathogen Listeria monocytogenes (Lm) causes invasive infection in susceptible animals and humans. To survive and proliferate within hosts, this facultative intracellular pathogen tightly coordinates the expression of a complex regulatory network that controls the expression of virulence factors. Here, we identified and characterized MouR, a novel virulence regulator of Lm. Through RNA-seq transcri...
Listeria monocytogenes CadC Regulates Cadmium Efflux and Fine-tunes Lipoprotein...
Pombinho, R; Camejo, A; Vieira, A; Reis, O; Carvalho, F; Almeida, MT; Pinheiro, JC; Sousa, S; Cabanes, DListeria monocytogenes is a major intracellular human foodborne bacterial pathogen. We previously revealed L. monocytogenes cadC as highly expressed during mouse infection. Here we show that L. monocytogenes CadC is a sequence-specific, DNA-binding and ca
L-Rhamnosylation of Listeria monocytogenes Wall Teichoic Acids Promotes Resista...
Carvalho, F; Atilano, ML; Pombinho, R; Covas, G; Gallo, RL; Filipe, SR; Sousa, S; Cabanes, DListeria monocytogenes is an opportunistic Gram-positive bacterial pathogen responsible for listeriosis, a human foodborne disease. Its cell wall is densely decorated with wall teichoic acids (WTAs), a class of anionic glycopolymers that play key roles in bacterial physiology, including protection against the activity of antimicrobial peptides (AMPs). In other Grampositive pathogens, WTA modification by amine-c...
Listeria monocytogenes induces host DNA damage and delays the host cell cycle t...
Leitão, E; Costa, AC; Brito, C; Costa, L; Pombinho, R; Cabanes, D; Sousa, SListeria monocytogenes (Lm) is a human intracellular pathogen widely used to uncover the mechanisms evolved by pathogens to establish infection. However, its capacity to perturb the host cell cycle was never reported. We show that Lm infection affects the host cell cycle progression, increasing its overall duration but allowing consecutive rounds of division. A complete Lm infectious cycle induces a S-phase del...