3 documents found, page 1 of 1
Linking genetic, metabolic, and phenotypic diversity among Saccharomyces cerevi...
Kang, Kang; Bergdahl, Basti; Machado, Daniel; Dato, Laura; Han, Ting-Li; Li, Jun; Villas-Boas, Silas; Herrgård, Markus J.; Förster, JochenThe selection of bioengineering platform strains and engineering strategies to improve the stress resistance of Saccharomyces cerevisiae remains a pressing need in bio-based chemical production. Thus, a systematic effort to exploit the genotypic and phenotypic diversity to boost yeast's industrial value is still urgently needed. Here, we analyzed 5400 growth curves obtained from 36 S. cerevisiae strains and com...
Metabolite secretion in microorganisms: the theory of metabolic overflow put to...
Pinu, Farhana R.; Granucci, Ninna; Daniell, James; Han, Ting-Li; Carneiro, Sónia; Rocha, I.; Nielsen, Jens; Villas-Boas, Silas G.Introduction Microbial cells secrete many metabolites during growth, including important intermediates of the central carbon metabolism. This has not been taken into account by researchers when modeling microbial metabolism for metabolic engineering and systems biology studies. Materials and Methods The uptake of metabolites by microorganisms is well studied, but our knowledge of how and why they secrete differ...
What is the relationship between intracellular and extracellular metabolites? T...
Villas-Boas, S.; Han, Ting-Li; Liu, Tim; Kim, Sang; Carneiro, S.; Ferreira, Eugénio C.; Rocha, I.Compared to our knowledge on metabolic pathways and the establishment of tools to manipulate these pathways, we know very little about the mechanisms behind the secretion of metabolic intermediates. Microorganisms secrete a wide range of metabolic intermediates, and many of them are of great industrial interest. Despite the cellular process of metabolite efflux being ubiquitous to all microbial cells, we still ...