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A sea of worms: the striking cases of the European Perinereis cultrifera and P....

Teixeira, Marcos André Machado Lima; Langeneck, Joachim; Grosse, Maël; Vieira, Pedro Emanuel Ferreira Reis; Hernández, José Carlos; Sampieri, Bruno R.

Molecular data have been suggesting the existence of a complex of cryptic species within the taxon Perinereis cultrifera, which has not been fully explored yet. In this study, we performed a morphological and molecular analysis (mtCOI-5P, 16S rRNA and 28SD2 rRNA) of Perinereis specimens from intertidal marine and brackish European localities, mostly focusing on the Mediterranean Sea and the Canary Islands. Two ...


A STEAM experience in the mathematics classroom: the role of a science cartoon

Marques, Daniela; Neto, Teresa B.; Guerra, Cecília; Viseu, Floriano; Aires, Ana Paula; Mota, Marina; Ravara, Ascensão

A multidisciplinary team collaborated on the development of a learning experience involving 10th grade students using a Science, Technology, Engineering, Arts, and Mathematics (STEAM) approach. The experience was based on the development (conception, implementation, and evaluation) of a science cartoon that aimed to highlight different scientific and technological dimensions related to the diversity of marine w...


Revealing the diversity of the green Eulalia (Annelida, Phyllodocidae) species ...

Teixeira, Marcos André Machado Lima; Vieira, Pedro Emanuel Ferreira Reis; Fenwick, David; Langeneck, Joachim; Pleijel, Fredrik; Sampieri, Bruno R.

The green phyllodocids Eulalia clavigera and E. viridis are a known European pseudo-cryptic complex, but questions about its distribution and evidence of additional lineages in previous studies call for an investigation of the real diversity within the complex. We analyze DNA sequences (mtCOI-5P, ITS, and 28S rRNA) of different populations of E. clavigera from intertidal and subtidal marine waters along the Nor...


Reappraisal of the hyperdiverse Platynereis dumerilii (Annelida: Nereididae) sp...

Teixeira, Marcos André Machado Lima; Langeneck, Joachim; Vieira, Pedro Emanuel Ferreira Reis; Hernández, José Carlos; Sampieri, Bruno R.

Morphologically similar species are often overlooked but molecular techniques have been effective in signalling potential hidden diversity, boosting the documentation of unique evolutionary lineages and ecological diversity. Platynereis dumerilii and Platynereis massiliensis are part of a recognised species complex, where differences in the reproductive biology have mainly been highlighted to date. Analyses of ...


The curious and intricate case of the European Hediste diversicolor (Annelida, ...

Teixeira, Marcos André Machado Lima; Bakken, Torkild; Vieira, Pedro Emanuel Ferreira Reis; Langeneck, Joachim; Sampieri, Bruno R.; Kasapidis, Panagiotis

Past molecular studies using mtDNA sequences and alloenzymes signalled the existence of at least two cryptic species within the Hediste diversicolor morphotype, in European coasts. However, to this day, no new species descriptions have been made. In this study, we identified five completely sorted lineages using a multi-locus approach, including the mitochondrial DNA cytochrome oxidase I gene (COI-5P) and the n...


From 13 to 22 in a second stroke: revisiting the European Eumida sanguinea (Phy...

Teixeira, Marcos A L; Vieira, Pedro E; Ravara, Ascensão; Costa, Filipe O.; Nygren, Arne

Eumida sanguinea is a recognized polychaete species complex which, in previous studies, has been reported to have additional undescribed diversity. We detected nine additional lineages by analysing DNA sequence data (mitochondrial: COI, 16S rRNA and nuclear loci: ITS region and 28S rRNA) of E. sanguinea morphotype populations from a broader sampling effort in European marine waters. Traditional morphological fe...


Molecular and morphometric analyses identify new lineages within a large Eumida...

Teixeira, Marcos André Machado Lima; Vieira, Pedro Emanuel Ferreira Reis; Pleijel, Fredrik; Sampieri, Bruno R.; Ravara, Ascensão; Costa, Filipe O.

We report on two new lineages of the Eumida sanguinea complex from Great Britain and describe one of them as a new species using a multilocus approach, including the mitochondrial DNA COI-5P and the nuclear markers ITS (ITS1, 5.8S rRNA and ITS2) and 28S rRNA. The molecular analysis placed Eumida mackiei sp. nov. in a monophyletic clade with 19.1% (COI), 10.1% (ITS) and 1.7% (28S) mean distance to its nearest ne...


Molecular and morphometric analyses identify new lineages within a large Eumida...

Teixeira, Marcos A. L.; Vieira, Pedro E.; Pleijel, Fredrik; Sampieri, Bruno R.; Ravara, Ascensão; Costa, Filipe O.; Nygren, Arne

We report on two new lineages of the Eumida sanguinea complex from Great Britain and describe one of them as a new species using a multilocus approach, including the mitochondrial DNA COI-5P and the nuclear markers ITS (ITS1, 5.8S rRNA and ITS2) and 28S rRNA. The molecular analysis placed Eumida mackiei sp. nov. in a monophyletic clade with 19.1% (COI), 10.1% (ITS) and 1.7% (28S) mean distance to its nearest ne...


Description of a new genus and species of Chrysopetalidae (Annelida: Polychaeta...

Ravara, Ascensão; Aguado, M. Teresa; Rodrigues, Clara F.; Génio, Luciana; Cunha, Marina R.

Five chrysopetalid species are reported from samples collected at bathyal depths in three NE Atlantic regions: the Bay of Biscay, the Horseshoe Abyssal Plain and the Gulf of Cadiz. Arichlidon reyssi (Katzmann et al., 1974), Dysponetus caecus (Langerhans, 1880) and D. profundus Böggemann, 2009 are free-living forms found mainly on biogenic substrates (e.g., coral and sunken wood). A brief description and taxonom...


Amphitrite fauveli sp. n. (Polychaeta: Terebellidae) from the Bay of Biscay and...

Jirkov, I. A.; Ravara, Ascensão; Cunha, Marina

A new species of Amphitrite is described from two different regions in the NE Atlantic: the Bay of Biscay and the Gulf of Cadiz. Amphitrite fauveli sp.n. differs from other Amphitrite species with cirriform branchiae mainly by having a smaller number of nephridial papillae (four pairs) and by lacking eyespots. In the Bay of Biscay, the new species occurred on the slope of two submarine canyons — Saint Nazaire (...


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