2 documents found, page 1 of 1
Direct PCR detection of foot-and-mouth disease virus
Rodriguez, A.; Nunez, J.; Nolasco, Gustavo; Ponz, F.; Sobrino, F.; Deblas, C.A PCR assay for the detection and characterization of foot-and-mouth disease virus was developed. The procedure allows RT-PCR amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. Using this procedure, FMDV-specific (based on 3D gene sequences), as well as serotype-specific (based on VP1 gene sequences) amplification were ac...
A method combining immunocapture and PCR amplification in a microtiter plate fo...
Nolasco, Gustavo; Deblas, C.; Torres, V.; Ponz, F.A method for the detection of RNA viral and subviral plant pathogens was developed that combines pathogen partial purification by solid-phase adsorbed antibodies, reverse transcriptional-polymerase chain reaction (RT-PCR) and quantitation of the amplified products by fluorescence. The reverse transcription of the RNA. is performed directly on the retained material without any previous thermal or chemical disrup...