22 documents found, page 1 of 3

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Evaluation of partial thromboplastin time, thrombin time and prothrombin time o...

Miranda, Vanessa Maria Andrade Machado; Barbosa Filho, José Pedro Martins; Costa, Romero Marcos Pedrosa Brandão; Leite, Ana Cristina Lima

Fibrinolytic proteases are enzymes that degrade the fibrin, a main component of the blood clot. Thus, the present study aimed to understand the evaluation of aPTT, TT and PT on plasma treated with a fibrinolytic protease produced by Mucor subtillissimus. To assess the anticoagulant effect, the protease was used in different concentrations of 0.5-2.5mg/mL. The tests showed that the enzyme promoted a significant ...

Date: 2022   |   Origin: Oasisbr

Effect of pH and temperature on phytase and biomass production by submerged fer...

Nascimento, Júlio Cézar dos Santos; Ribeiro, Apolônio Gomes; Pessoa, Ricardo Alexandre Silva; Rabello, Carlos Bôa Viagem; Venâncio, Armando

The production of phytase and biomass (estimated by the ergosterol content) by submerged fermentation with Aspergillus niger var. phoenicis URM 4924 was studied. Experimental assays were carried under different conditions of pH (4.0 to 8.0) and temperature (25 to 35 ºC), and the influence of these variables on the responses was studied through a 22 central composite design and response surface methodology. Phyt...

Date: 2022   |   Origin: Oasisbr

Microalgal production under mixotrophic conditions using cheese whey as substrate

Andrade, Alexsandra Frazao de; Silva, Páblo Eugênio da Costa e; Melo, Rebeca Gonçalves de; Ferreira, Millena Patrício do Nascimento

Microalgae are known for producing various biotechnological products. Moreover, they absorb nutrients from dairy wastewater, grow well, and accumulate valuable compounds faster. In this study, photoautotrophic and mixotrophic cultivation with different initial lactose concentrations present in cheese whey (CW) were established to investigate their effect on cell concentration (Xm, mg L-1), cell productivity (Px...

Date: 2022   |   Origin: Oasisbr

The effect of exopolysaccharides obtained from lactic acid bacteria as a prebio...

Monteiro, Rejane Gonçalves; Silva, Elaine Cristina da; Martins, Anna Larissa Cerqueira; Porto, Ana Lúcia Figueiredo

Exopolysaccharides from lactic acid bacteria are biopolymers known for their structural diversity and because of this they have several beneficial effects on human health, and can act as immunomodulators, antioxidants, antitumors etc. It is known that exopolysaccharides also help in the balance of the intestinal microbiota, but there is still no evidence that it is due to a prebiotic effect. Therefore, this stu...

Date: 2021   |   Origin: Oasisbr

Lactic acid bacteria isolated from Coalho cheese from northeast Brazil in dairy...

Brito, Leandro Paes de; Silva, Elaine Cristina da; Calaça, Priscilla Régia de Andrade; Medeiros, Rosália Severo de

In Brazil, dairy production is the second most important segment of the food industry, due to its nutritional and sensory characteristics. Lactic cultures are used in production technology to accentuate the flavor, aroma, texture, and acidity of dairy products that make them more acceptable to consumers. Therefore, the objective of this study was to evaluate the technological potential of species of the genus E...

Date: 2020   |   Origin: Oasisbr

Purification of a lectin from Cratylia mollis crude extract seed by a single st...

Nascimento, Cynthia Oliveira; Costa, Romero Marcos Pedrosa Brandão; Soares, Paulo Antônio; Herculano, Polyanna Nunes; Porto, Tatiana Souza

The partitioning and purification of lectins from the crude extract of Cratylia mollis seeds (Cramoll 1,4) was investigated in aqueous two-phase systems (ATPS). A factorial design model (24) was used to evaluate the influence of polyethylene glycol (PEG) molar mass (15008000g/mol), PEG concentration (12.517.5% w/w), phosphate (1015% w/w) concentration, and pH (68) on the differential partitioning, purification ...


Process development for the production of prebiotic fructo-oligosaccharides by ...

Gonçalves, Clarisse Salomé Nobre; Nascimento, A. K. C.; Silva, Soraia Pires; Coelho, Elisabete; Coimbra, Manuel A.; Cavalcanti, Maria Taciana Holanda

A new isolated P. citreonigrum URM 4459 was selected to produce fructooligosaccharides (FOS) in an efficient, economical and fast one-step fermentation. Optimal culture conditions were stablished by experimental design. Experiments run in bioreactor resulted in a high yield, content, productivity and purity of FOS (0.65 ± 0.06 gFOS.ginitial sucrose-1, 126.3 ± 0.1 g/L, 2.28 ± 0.08 g/L.h and 61 ± 0 %). The FOS mi...


Brazilian kefir-fermented sheep's milk, a source of antimicrobial and antioxida...

de Lima, Meire dos Santos Falcão; da Silva, Roberto Afonso; da Silva, Milena Fernandes; da Silva, Paulo Alberto Bezerra

Fermented milks are a source of bioactive peptides and may be considered as functional foods. Among these, sheep's milk fermented with kefir has not been widely studied and its most relevant properties need to be more thoroughly characterized. This research study is set out to investigate and evaluate the antioxidant and antimicrobial properties of peptides from fermented sheep's milk in Brazil when produced by...


Metaloproteinases: aspectos fisiopatológicos sistêmicos e sua importância na ci...

Araújo, Rosangela Vidal de Souza; Silva, Flávio Oliveira; Melo-Junior, Mário Ribeiro de; Porto, Ana Lúcia Figueiredo; Araújo, Rosangela Vidal de Souza

Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2017-06-05T18:51:35Z No. of bitstreams: 1 14_v.10_1.pdf: 453704 bytes, checksum: fd089dcbe4a5f1391a48afb115b97caa (MD5); Made available in DSpace on 2017-06-05T18:51:35Z (GMT). No. of bitstreams: 1 14_v.10_1.pdf: 453704 bytes, checksum: fd089dcbe4a5f1391a48afb115b97caa (MD5) Previous issue date: 2011-01; O objetivo deste trabalho foi descrev...

Date: 2017   |   Origin: Oasisbr

Collagenolytic enzymes produced by fungi: a systematic review

Wanderley, M.; Neto, José Manoel Werley Duarte; Filho, José Luiz de Lima; Lima, Carolina de Albuquerque; Teixeira, J. A.; Porto, Ana Lúcia Figueiredo

Specific proteases capable of degrading native triple helical or denatured collagen have been required for many years and have a large spectrum of applications. There are few complete reports that fully uncover production, characterization and purification of fungi collagenases. In this review, authors searched through four scientific on line data bases using the following keywords (collagenolytic OR collagenas...


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