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Understanding the tolerance of different strains of human pathogenic bacteria t...

Figueira, Priscilla Porto; Câmara, José S.; Vigário, Ana M.; Pereira, Jorge A. M.; Porto-Figueira, Priscilla; Câmara, José

<jats:p>It is known that many bacteria are susceptible to low pH, but it is less clear how the acid nature influences this susceptibility. The objective of this work was to evaluate the susceptibility of selected bacteria to different low pH conditions and to understand if their tolerance is affected by the type of acid (organic vs inorganic) in the media acidification. Strains of different species of human pat...


Urinary Volatomic Expression Pattern: Paving the Way for Identification of Pote...

Taunk, Khushman; Porto-Figueira, Priscilla; Pereira, Jorge A. M.; Taware, Ravindra; Costa, Nattane Luíza da; Barbosa, Rommel; Rapole, Srikanth

The urinary volatomic profiling of Indian cohorts composed of 28 lung cancer (LC) pa tients and 27 healthy subjects (control group, CTRL) was established using headspace solid phase microextraction technique combined with gas chromatography mass spectrometry methodology as a powerful approach to identify urinary volatile organic metabolites (uVOMs) to discriminate among LC patients from CTRL. Overall, 147 VOMs ...


The potential of microextraction techniques for the analysis of bioactive compo...

Pereira, Jorge A. M.; Casado, Natalia; Porto-Figueira, Priscilla; Câmara, José S.

For a long time, the importance of sample preparation and extraction in the analytical performance of the most diverse methodologies have been neglected. Cumbersome techniques, involving high sample and solvent volumes have been gradually miniaturized from solid-phase and liquid-liquid extractions formats and microextractions approaches are becoming the standard in different fields of research. In this context,...


Profiling the occurrence of biogenic amines in different types of tuna samples ...

Pataca, Joanna K.G.; Porto-Figueira, Priscilla; Pereira, Jorge A. M.; Caldeira, Helena; Câmara, José S.

Food deterioration caused by microbial agents often involve the formation of biogenic amines (BAs), which can have harmful effects on human health. In this study a set of BAs - tryptamine, cadaverine, putrescine, spermine, histamine, tyramine, and spermidine, were simultaneously analysed to monitor their occurrence in different types of tuna samples. An improved extraction approach involving ultrasound-assisted...


Evaluation of the Health-Promoting Properties of Selected Fruits

Figueira, José A.; Porto-Figueira, Priscilla; Berenguer, Cristina; Pereira, Jorge A. M.; Câmara, José S.

In this study, the health-promoting benefits of different fruits grown in Madeira Island, namely lemon (Citrus limon var. eureka), tangerine (Citrus reticulata var. setubalense), pitanga (Eugenia uniflora var. red), tomato (Solanum lycopersicum var. gordal) and uva-da-serra, an endemic blueberry (Vaccinium padifolium Sm.), were investigated. The phenolic composition (total phenolics and total flavonoids content...


The salivary volatome in breast cancer

Pereira, Jorge A. M.; Taware, Ravindra; Porto-Figueira, Priscilla; Rapole, Srikanth; Câmara, José S.


Unravelling the potential of salivary volatile metabolites in oral diseases. A ...

Pereira, Jorge A. M.; Porto-Figueira, Priscilla; Taware, Ravindra; Sukul, Pritam; Rapole, Srikanth; Câmara, José S.

Fostered by the advances in the instrumental and analytical fields, in recent years the analysis of volatile organic compounds (VOCs) has emerged as a new frontier in medical diagnostics. VOCs analysis is a non-invasive, rapid and inexpensive strategy with promising potential in clinical diagnostic procedures. Since cellular metabolism is altered by diseases, the resulting metabolic effects on VOCs may serve as...


Tangerines cultivated on Madeira Island: a high throughput natural source of bi...

Figueira, José A.; Porto-Figueira, Priscilla; Pereira, Jorge A. M.; Câmara, José S.

Tangerines (Citrus reticulata) are popular fruits worldwide, being rich in many bioactive metabolites. The setubalense variety cultivated on Madeira Island has an intense aroma easily distinguishable from other tangerines, being traditionally used to enrich several foods and beverages. Nonetheless, setubalense volatile composition has never been characterized, and we aimed to unveil the bioactive potential of p...


A comprehensive methodology based on NTME/GC-MS data and chemometric tools for ...

Figueira, José; Porto-Figueira, Priscilla; Pereira, Jorge A. M.; Câmara, José S.

In this work we report an innovative and high throughput methodology involving Needle Trap Microextraction (NTME) combined with GC-MS analyis and chemometric processing, to obtain comprehensive volatile finger prints for authenticity purposes. This approach ewill allow to characterize the volatile composition of lemon peels (exocarp) (Eureka variety) from different geographical regions of Portugal (mainland and...


Current trends on microextraction by packed sorbent – fundamentals, application...

Pereira, Jorge A. M.; Gonçalves, João; Porto-Figueira, Priscilla; Figueira, José A.; Alves, Vera; Perestrelo, Rosa; Medina, Sonia; Câmara, José S.

MEPS, the acronym of microextraction by packed sorbent, is a simple, fast and user- and environmen tally-friendly miniaturization of the popular solid-phase extraction technique (SPE). In fact, it has been widely shown that MEPS can easily replace SPE for most, if not all, previous applications. It can attain this with obvious gains in sample and solvent usage, which is greatly reduced without compromising the ...


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