22 documents found, page 1 of 3
Genomic-based surveillance reveals high ongoing transmission of multi-drug-resi...
Salvato, Richard Steiner; Reis, Ana Júlia; Schiefelbein, Sun Hee; Gómez, Michael Andrés Abril; Salvato, Stéphanie Steiner; da Silva, Larissa VitóriaFunding Information: Funding: This study was funded, in part, by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (Grant No. 001) and supported by Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (Grant No. 17/1265–8 INCT-TB). JP is supported by Fundação para a Ciência e Tecnologia through Estímulo Individual ao Emprego Científico (CEECIND/00394/2017). Funding Information: Th...
Genetic diversity of candidate loci linked to Mycobacterium tuberculosis resist...
Gómez-González, Paula J.; Perdigao, Joao; Gomes, Pedro; Puyen, Zully M.; Santos-Lazaro, David; Napier, Gary; Hibberd, Martin L.; Viveiros, MiguelFunding Information: PJG-G is funded by an MRC-LID PhD studentship. JEP is funded by a Newton Institutional Links Grant (British Council, no. 261868591). TGC is funded by the Medical Research Council UK (Grant no. MR/M01360X/1, MR/ N010469/1, MR/R025576/1, and MR/R020973/1) and BBSRC (Grant no. BB/R013063/1). SC is funded by Medical Research Council UK grants (ref. MR/M01360X/1, MR/R025576/1, and MR/R020973/1)....
Author Correction
Verza, Mirela; Scheffer, Mara Cristina; Salvato, Richard Steiner; Schorner, Marcos André; Barazzetti, Fernando Hartmann; de Melo Machado, HanalydiaAn amendment to this paper has been published and can be accessed via a link at the top of the paper.
Using genomics to understand the origin and dispersion of multidrug and extensi...
Perdigão, João; Gomes, Pedro; Miranda, Anabela; Maltez, Fernando; Machado, Diana; Silva, Carla; Phelan, Jody E.; Brum, Laura; Campino, SusanaPortugal is a low incidence country for tuberculosis (TB) disease. Now figuring among TB low incidence countries, it has since the 1990s reported multidrug resistant and extensively drug resistant (XDR) TB cases, driven predominantly by two strain-types: Lisboa3 and Q1. This study describes the largest characterization of the evolutionary trajectory of M/XDR-TB strains in Portugal, spanning a time-period of two...
Genomic epidemiology of Mycobacterium tuberculosis in Santa Catarina, Southern ...
Verza, Mirela; Scheffer, Mara Cristina; Salvato, Richard Steiner; Schorner, Marcos André; Barazzetti, Fernando Hartmann; Machado, Hanalydia de MeloMycobacterium tuberculosis (M.tb), the pathogen responsible for tuberculosis (TB) poses as the major cause of death among infectious diseases. The knowledge about the molecular diversity of M.tb enables the implementation of more effective surveillance and control measures and, nowadays, Whole Genome Sequencing (WGS) holds the potential to produce high-resolution epidemiological data in a high-throughput manner...
Genome-wide analysis of Mycobacterium tuberculosis polymorphisms reveals lineag...
Oppong, Yaa E. A.; Phelan, Jody; Perdigao, Joao; Machado, Diana; Miranda, Anabela; Portugal, Isabel; Viveiros, Miguel; Clark, Taane G.Background: Continuing evolution of the Mycobacterium tuberculosis (Mtb) complex genomes associated with resistance to anti-tuberculosis drugs is threatening tuberculosis disease control efforts. Both multi- and extensively drug resistant Mtb (MDR and XDR, respectively) are increasing in prevalence, but the full set of Mtb genes involved are not known. There is a need for increased sensitivity of genome-wide ap...
CPLP-TB:
Perdigão, João; Silva, Carla; Diniz, Jaciara; Pereira, Catarina; Machado, Diana; Ramos, Jorge; Silva, Hugo; Abilleira , Fernanda; Brum, ClariceA Tuberculose (TB) permanece um grave problema de saúde pública na Comunidade dos Países de Língua Portuguesa (CPLP). Apesar da ampla variância da incidência da TB nos seus estados-membro e de um fluxo migratório contínuo entre os países que integram este grupo, existe uma enorme lacuna no que diz respeito ao conhecimento da estrutura populacional conjunta do Mycobacterium tuberculosis e circulação de estirpes ...
Global expansion of Mycobacterium tuberculosis lineage 4 shaped by colonial mig...
Brynildsrud, Ola B.; Pepperell, Caitlin S.; Suffys, Philip; Grandjean, Louis; Monteserin, Johana; Debech, Nadia; Bohlin, Jon; Alfsnes, KristianOn the basis of population genomic and phylogeographic analyses of 1669 Mycobacterium tuberculosis lineage 4 (L4) genomes, we find that dispersal of L4 has been completely dominated by historical migrations out of Europe. We demonstrate an intimate temporal relationship between European colonial expansion into Africa and the Americas and the spread of L4 tuberculosis (TB). Markedly, in the age of antibiotics, m...
Methylation in Mycobacterium tuberculosis is lineage specific with associated m...
Phelan, Jody; De Sessions, Paola Florez; Tientcheu, Leopold; Perdigao, Joao; Machado, Diana; Hasan, Rumina; Hasan, Zahra; Bergval, Indra L.DNA methylation is an epigenetic modification of the genome involved in regulating crucial cellular processes, including transcription and chromosome stability. Advances in PacBio sequencing technologies can be used to robustly reveal methylation sites. The methylome of the Mycobacterium tuberculosis complex is poorly understood but may be involved in virulence, hypoxic survival and the emergence of drug resist...
Efflux Activity Differentially Modulates the Levels of Isoniazid and Rifampicin...
Machado, Diana; Perdigao, Joao; Portugal, Isabel; Pieroni, Marco; Silva, Pedro A.; Couto, Isabel; Viveiros, MiguelWith the growing body of knowledge on the contribution of efflux activity to Mycobacterium tuberculosis drug resistance, increased attention has been given to the use of efflux inhibitors as adjuvants of tuberculosis therapy. Here, we investigated how efflux activity modulates the levels of efflux between monoresistant and multi- and extensively drug resistant (M/XDR) M. tuberculosis clinical isolates. The stra...