8 documents found, page 1 of 1
Emergence of Multidrug-Resistant Mycobacterium Tuberculosis of the Beijing Line...
Perdigão, J; Silva, C; Maltez, F; Machado, D; Miranda, A; Couto, I; Rabna, P; Florez de Sessions, P; Phelan, J; Pain, A; McNerney, R; Hibberd, MThe Beijing genotype comprises a highly disseminated strain type that is frequently associated with multidrug resistant (MDR) tuberculosis (TB) and increased transmissibility but, countries such as Portugal and Guinea-Bissau fall outside the regions phylogeographically associated with this specific genotype. Nevertheless, recent data shows that this genotype might be gradually emerging in these two countries as...
Supercritical CO2 extraction of Aurantiochytrium sp. biomass for the enhanced r...
Melo, M.M.R. de; Sapatinha, M.; Pinheiro, J.; Lemos, M.F.L.; Bandarra, N.M.; Batista, I.; Paulo, M.C.; Coutinho, J.; Saraiva, J.A.; Portugal, IThe microalgae Aurantiochytrium sp. is a strong alternative source of ω-3 fatty acids, including ocosahexaenoic acid (DHA). This work encompasses the optimization of SFE conditions to maximize the total extraction yield (ηTotal), DHA content (CDHA), total phenolics content (TPC), and antioxidant capacity (AOC) of the extracts produced from Aurantiochytrium sp. biomass. A full factorial experimental plan was per...
Using Genomics to Understand the Origin and Dispersion of Multidrug and Extensi...
Perdigão, J; Gomes, P; Miranda, A; Maltez, F; Machado, D; Silva, C; Phelan, J; Brum, L; Campino, S; Couto, I; Viveiros, M; Clark, T; Portugal, IPortugal is a low incidence country for tuberculosis (TB) disease. Now figuring among TB low incidence countries, it has since the 1990s reported multidrug resistant and extensively drug resistant (XDR) TB cases, driven predominantly by two strain-types: Lisboa3 and Q1. This study describes the largest characterization of the evolutionary trajectory of M/XDR-TB strains in Portugal, spanning a time-period of two...
Tuberculose Multirresistente por Estirpes da Família Beijing, em Doentes de Lis...
Maltez, F; Martins, T; Póvoas, D; Cabo, J; Peres, H; Antunes, F; Perdigão, J; Portugal, IIntrodução: As estirpes de Mycobacterium tuberculosis da família Beijing estão associadas à multirresistência. As estirpes da família Lisboa prevalecem entre os doentes com tuberculose multirresistente e extensivamente resistente desta região, mas vários estudos documentam a presença da família Beijing, embora desconhecendo-se as características dos doentes donde foram isoladas. Material e Métodos: Estudo retro...
Recombination in pe/ppe genes contributes to genetic variation in Mycobacterium...
Phelan, JE; Coll, F; Bergval, I; Anthony, RM; Warren, R; Sampson, SL; Gey van Pittius, NC; Glynn, JR; Crampin, AC; Alves, A; Bessa, TB; Campino, SBackground: Approximately 10% of the Mycobacterium tuberculosis genome is made up of two families of genes that are poorly characterized due to their high GC content and highly repetitive nature. The PE and PPE families are typified by their highly conserved N-terminal domains that incorporate proline-glutamate (PE) and proline-proline-glutamate (PPE) signature motifs. They are hypothesised to be important viru...
Alkenes oligomerization with resin catalysts
Antunes, BM; Alírio Rodrigues; Lin, Z; Portugal, I; Silva, CMThe growing interest of chemical and oil refining industries in the catalytic oligomerization of alkenes for the production of synthetic fuels and additives formulated around light gasoline olefins is due to recent environmental restrictions and increasing diesel demand. Furthermore, the drive towards sustainability and the emergent contribution of biorefineries for the manufacture of intermediate and platform ...
pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis isolates in...
Portugal, I; Barreiro, L; Moniz-Pereira, J; Brum, LThe nucleotide sequences of the pncA genes within 55 multidrug-resistant pyrazinamide-resistant Mycobacterium tuberculosis clinical isolates were determined. Fifty-three out of the 55 isolates were pyrazinamidase (PZase) negative. Four strains contained a
Isoniazid-induced transient high-level resistance in Mycobacterium tuberculosis
Viveiros, M; Portugal, I; Bettencourt, R; Victor, TC; Jordaan, AM; Leandro, C; Ordway, D; Amaral, LAn American Type Culture Collection reference strain and eight clinical strains of Mycobacterium tuberculosis, all of which were susceptible to isoniazid (INH) (mean MIC, 0.06 mg/liter) and negative for the Ser315Thr katG mutation, were left in their BACTEC 12B vials (for use with the BACTEC 460-TB method) containing 0.1 mg of INH per liter for periods of up to 28 days after the completion of the antibiotic sus...