50 documents found, page 1 of 5

Emergence of KPC-3- and OXA-181-producing ST13 and ST17 Klebsiella pneumoniae i...

Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) strains are of particular concern, especially strains with mobilizable carbapenemase genes such as blaKPC, blaNDM or blaOXA-48, given that carbapenems are usually the last line drugs in the β-lactam class and, resistance to this sub-class is associated with increased mortality and frequently co-occurs with resistance to other antimicrobial classes. Ob...

Spread of Mycobacterium tuberculosis in Southern Brazilian persons deprived of ...

To evaluate the genetic diversity and clustering rates of M. tuberculosis strains to better understand transmission among persons deprived of liberty (PDL) in Rio Grande do Sul (RS), southern Brazil. This is a cross-sectional study, including strains of M. tuberculosis isolated from PDL, stored at the Central Laboratory of RS, in the period from 2013 to 2018. The molecular characterization was performed using t...

Synthesis, Characterization and Complex Evaluation of Antibacterial Activity an...

The synthesis of 20 arylidenecamphors and 15 pyrimidines with camphane skeleton is described in the current report. A modified method for preparation of sterically hindered 2-aminopyrimidines in two steps was demonstrated. The evaluation of their in vitro activity against Mycobacterium tuberculosis H37Rv showed different MIC values (up to 0.91 μM for ketone 39). Compound 35 demonstrated moderate (8.23 μM), but ...

Molecular Insight into Mycobacterium tuberculosis Resistance to Nitrofuranyl Am...

We performed synthesis of new nitrofuranyl amides and investigated their anti-TB activity and primary genetic response of mycobacteria through whole-genome sequencing (WGS) of spontaneous resistant mutants. The in vitro activity was assessed on reference strain Mycobacterium tuberculosis H37Rv. The most active compound 11 was used for in vitro selection of spontaneous resistant mutants. The same mutations in si...

Whole-genome sequencing as a tool for studying the microevolution of drug-resis...

Treatment of drug-resistant tuberculosis requires extended use of more toxic and less effective drugs and may result in retreatment cases due to failure, abandonment or disease recurrence. It is therefore important to understand the evolutionary process of drug resistance in Mycobacterium tuberculosis. We here in describe the microevolution of drug resistance in serial isolates from six previously treated patie...

Genomic-based surveillance reveals high ongoing transmission of multi-drug-resi...

Genomic-based surveillance on the occurrence of drug resistance and its transmission dynamics has emerged as a powerful tool for the control of tuberculosis (TB). A whole-genome sequencing approach, phenotypic testing and clinical-epidemiological investigation were used to undertake a retrospective population-based study on drug-resistant (DR)-TB in Rio Grande do Sul, the largest state in Southern Brazil. The a...

Genetic diversity of candidate loci linked to Mycobacterium tuberculosis resist...

Tuberculosis (TB), caused by Mycobacterium tuberculosis, is one of the deadliest infectious diseases worldwide. Multidrug and extensively drug-resistant strains are making disease control difficult, and exhausting treatment options. New anti-TB drugs bedaquiline (BDQ), delamanid (DLM) and pretomanid (PTM) have been approved for the treatment of multi-drug resistant TB, but there is increasing resistance to them...

Expanded tracking of a Beijing Mycobacterium tuberculosis strain involved in an...

Phylogenomic perspective on a unique mycobacterium bovis clade dominating bovin...

Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior ? Brazil (CAPES), Instituto Evandro Chagas/MS/SVS and Funda??o para o desenvolvimento cient?fco e tecnol?gico em sa?de [PRES-012-FIO-16]; CAPES ? Finance Code 001, [194?88881.187587/2018?01, 2018].; German Center for Infection Research (DZIF). European Society of Clinical Microbiology and Infectious Diseases, for which we would like to would like to ac...

Using genomics to understand the origin and dispersion of multidrug and extensi...

Portugal is a low incidence country for tuberculosis (TB) disease. Now figuring among TB low incidence countries, it has since the 1990s reported multidrug resistant and extensively drug resistant (XDR) TB cases, driven predominantly by two strain-types: Lisboa3 and Q1. This study describes the largest characterization of the evolutionary trajectory of M/XDR-TB strains in Portugal, spanning a time-period of two...