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Making waves

Alygizakis, Nikiforos; Ng, Kelsey; Čirka, Ľuboš; Berendonk, Thomas; Cerqueira, Francisco; Cytryn, Eddie; Deviller, Geneviève; Fortunato, Gianuario

With the global concerns on antibiotic resistance (AR) as a public health issue, it is pivotal to have data exchange platforms for studies on antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in the environment. For this purpose, the NORMAN Association is hosting the NORMAN ARB&ARG database, which was developed within the European project ANSWER. The present article provides an overview...


Mitigating risks and maximizing sustainability of treated wastewater reuse for ...

Yalin, David; Craddock, Hillary A.; Assouline, Shmuel; Ben Mordechay, Evyatar; Ben-Gal, Alon; Bernstein, Nirit; Chaudhry, Rabia M.; Chefetz, Benny

Scarcity of freshwater for agriculture has led to increased utilization of treated wastewater (TWW), establishing it as a significant and reliable source of irrigation water. However, years of research indicate that if not managed adequately, TWW may deleteriously affect soil functioning and plant productivity, and pose a hazard to human and environmental health. This review leverages the experience of research...


Impact of disinfection processes on bacterial community in urban wastewater: Sh...

Cesare, Andrea Di; Corno, Gianluca; Manaia, Célia M.; Rizzo, Luigi

The development of new methods and technologies for microbial characterization as well as their increasing availability at more affordable costs, has made evident the limitations of the conventional and routinely applied (typically cultivation based) methods to exhaustively and fully characterize the actual effect of disinfection process in urban wastewater treatment plants (UWWTPs). Such problem is even more r...


Immobilised cerium-doped Zinc oxide as a photocatalyst for the degradation of a...

Zammit, Ian; Vaiano, Vincenzo; Ribeiro, Ana R.; Silva, Adrián M. T.; Manaia, Célia M.; Rizzo, Luigi

The threat of antibiotic resistance to the wellbeing of societies is well established. Urban wastewater treatment plants (UWTPs) are recognised sources for antibiotic resistance dissemination in the environment. Herein a novel cerium-doped zinc oxide (Ce-ZnO) photocatalyst is compared to ZnO and the benchmark TiO2-P25 in the immobilised form on a metallic support, to evaluate a photocatalytic process as a possi...


Performance of secondary wastewater treatment methods for the removal of contam...

Krzeminski, Pawel; Tomei, Maria Concetta; Karaolia, Popi; Langenhoff, Alette; Almeida, C. Marisa R.; Felis, Ewa; Gritten, Fanny; Andersen, Henrik Rasmus

Contaminants of emerging concern (CEC) discharged in effluents of wastewater treatment plants (WWTPs), not specifically designed for their removal, pose serious hazards to human health and ecosystems. Their impact is of particular relevance to wastewater disposal and re-use in agricultural settings due to CEC uptake and accumulation in food crops and consequent diffusion into the food-chain. This is the reason ...


Antibiotic resistance genes in treated wastewater and in the receiving water bo...

Cacace, Damiano; Fatta-Kassinos, Despo; Manaia, Célia M.; Cytryn, Eddie; Kreuzinger, Norbert; Rizzo, Luigi; Karaolia, Popi; Schwartz, Thomas

There is increasing public concern regarding the fate of antibiotic resistance genes (ARGs) during wastewater treatment, their persistence during the treatment process and their potential impacts on the receiving water bodies. In this study, we used quantitative PCR (qPCR) to determine the abundance of nine ARGs and a class 1 integron associated integrase gene in 16 wastewater treatment plant (WWTP) effluents f...


High throughput analysis of integron gene cassettes in wastewater environments

Gatica, Joao; Tripathi, Vijay; Green, Stefan; Manaia, Célia M.; Berendonk, Thomas; Cacace, Damiano; Merlin, Christophe; Kreuzinger, Norbert

Integrons are extensively targeted as a proxy for anthropogenic impact in the environment. We developed a novel high-throughput amplicon sequencing pipeline that enables characterization of thousands of integron gene cassette-associated reads, and applied it to acquire a comprehensive overview of gene cassette composition in effluents from wastewater treatment facilities across Europe. Between 38 100 and 172 99...


Vancomycin resistant enterococci: from the hospital effluent to the urban waste...

Varela, Ana Rita; Ferro, Giovanna; Vredenburg, Jana; Yanik, Melike; Vieira, Lucas; Rizzo, Luigi; Lameiras, Catarina; Manaia, Célia M.

Vancomycin is an important antibiotic to treat serious nosocomial enterococci infections. Human activities, in particular those related with clinical practices performed in hospitals, can potentiate the transfer and selection of clinically-relevant resistant bacteria such as vancomycin resistant enterococci (VRE). Indeed, previous studies demonstrated the occurrence of VRE in urban wastewater treatment plants a...


Vancomycin and ciprofloxacin resistance in enterococci from a Hospital effluent...

Varela, Ana Rita; Ferro, Giovanna; Vredenburg, Jana; Yanik, Melike; Rizzo, Luigi; Manaia, Célia M.


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