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Seasonal variation of chub mackerel (Scomber colias) selenium and vitamin B12 c...

Rego, A.; Coelho, I.; Motta, C.; Cardoso, C.; Gomes-Bispo, A.; Afonso, C.; Prates, J.A.M.; Bandarra, N.M.; Silva, J.A.L.; Castanheira, I.

Chub mackerel (Scomber colias), an underutilised fish species, has been identified as an excellent source of selenium and vitamin B12, which may prevent and delay cognitive decline and dementia. Given these nutrients’ contents seasonal variability, a complete monthly sampling, involving a specifically adjusted methodology for the determination of vitamin B12 (LC-MS/MS) and its main vitamers and a sensitive anal...


Seasonality as experienced in the market and the resulting variation in the ami...

Motta, C.; Rego, A.; Cardoso, C.; Coelho, I.; Gomes-Bispo, A.; Afonso, C.; Prates, J.A.M.; Castanheira, I.; Bandarra, N.

Chub mackerel (Scomber colias) is an underutilised fish species, abundant in the Atlantic Ocean. Its amino acid profile and elemental composition are still insufficiently studied. Accordingly, these aspects were studied on a monthly basis. The most abundant essential amino acids, lysine and leucine, had contents ranging from 1270 ± 40 mg/100 g in August to 1820 ± 96 mg/100 g in November and from 1210 ± 36 mg/10...


Influence of dietary Chlorella vulgaris and carbohydrate-active enzymes on grow...

Alfaia, C.M.; Pestana, J.M.; Rodrigues, M.; Coelho, D.; Aires, M.J.; Ribeiro, D.M.; Major, V.T.; Martins, C.F.; Santos, H.; Lopes, P.A.; Lemos, J.P.C.

Herein, we investigated the effect of Chlorella vulgaris as ingredient (10% of incorporation) in broiler diets, supplemented or not with 2 formulations of Carbohydrate-Active enZymes (CAZymes; Rovabio Excel AP and a mixture of recombinant CAZymes, composed by an exo-b-glucosaminidase, an alginate lyase, a peptidoglycan N-acetylmuramic acid deacetylase and a lysozyme), on growth performance, meat quality, fatty ...


Impact of dietary incorporation of Spirulina (Arthrospira platensis) and exogen...

Pestana, J.M.; Puerta, B.; Santos, H.; Madeira, M.S.; Alfaia, C.M.; Lopes, P.A.; Pinto, R.M.A.; Lemos, J.P.C.; Fontes, C.M.G.A.; Lordelo, M.M.

This study assessed the effect of Spirulina (Arthrospira platensis), individually and in combination with exogenous enzymes, on growth performance, carcass traits, and meat quality of broiler chickens. One hundred and twenty Ross 308 male chickens were allocated into 40 battery brooders, with 3 birds per cage, and fed ad libitum a corn-based diet during the first 21 D of the trial. The experimental period laste...


The effects of restricting enzyme supplementation in rye-based diets for broilers

Santos, C.I.; Ribeiro, T.; Ponte, P.I.P.; Fernandes, V.O.; Falcão-Cunha, L.; Freire, J.P.B.; Prates, J.A.M.; Ferreira, L.M.A.; Fontes, C.M.G.A.

The purpose of the current study was to restrict enzyme addition in order to evaluate if supplementation throughout the broiler production cycle is required. Here we analyze the possibility of circumscribing xylanase supplementation in rye-based diets to the earliest periods of the life of the broiler. In the current experiment, 1-day-old chicks were divided into 5 treatments and fed a rye-based diet supplement...


The effects of restricting enzyme supplementation in wheat-based diets to broilers

Figueiredo, A.A.; Correia, B.A.; Ribeiro, T.; Ponte, P.I.P.; Falcão-Cunha, L.; Freire, J.P.B.; Prates, J.A.M.; Ferreira, L.M.A.; Fontes, C.M.G.A.

The performance of broilers fed on xylanase supplemented diets for part or all of the production cycle was evaluated in two experiments. In a preliminary experiment, 1-day-old chicks were fed a wheat-based diet supplemented with a commercial xylanase for the entire duration of the experiment of 28 days, or during the last 21, 14, or 7 days of the trial. In experiment 2, the growth period was extended to 36 days...


A PRND polymorphism in Churra do Campo portuguese sheep breed

Mesquita, P.; Silva, M.F.S.; Carolino, M.C.; Sousa, M.C.O.; Pimenta, J.; Marques, M.R.; Santos, I.C.; Gama, L.T.; Andrade, C.S.C.R.; Fontes, C.M.

Prion-like Doppel gene (PRND) is located downstream from priori protein gene (PRNP). Doppel protein is not related to prion disease but to male fertility. Our previous analysis of PRND coding region in 460 animals from 8 Portuguese sheep breeds, by multiple restriction fragment-single strand conformation polymorphism (MRFSSCP), revealed a synonymous substitution (78G>A). An association was found between identif...


A family 11 carbohydrate binding module (CBM) improves the efficacy of a recomb...

Lordelo, M.M.; Ribeiro, T.; Ponte, P.I.P.; Guerreiro, C.I.P.D.; Santos, H.M.; Falcão, L.; Freire, J.P.B.; Ferreira, L.M.A.; Prates, J.A.M.

1. Exogenous microbial -1,3-1,4-glucanases and hemicellulases contribute to improving the nutritive value of cereals rich in soluble non-starch polysaccharides for poultry. 2. In general, plant cell wall hydrolases display a modular structure comprising a catalytic module linked to one or more non-catalytic carbohydrate-binding modules (CBMs). Based on primary structure similarity, CBMs have been classified in ...


A family 11 carbohydrate-binding module (CBM) improves the efficacy of a recomb...

Ribeiro, T.; Ponte, P.I.P.; Guerreiro, C.I.P.D.; Santos, H.M.; Falcão, L.; Freire, J.P.B.; Ferreira, L.M.A.; Prates, J.A.M.; Fontes, C.M.G.A.

1. Exogenous microbial -1,3-1,4-glucanases and hemicellulases contribute to improving the nutritive value of cereals rich in soluble non-starch polysaccharides for poultry. 2. In general, plant cell wall hydrolases display a modular structure comprising a catalytic module linked to one or more non-catalytic carbohydrate-binding modules (CBMs). Based on primary structure similarity, CBMs have been classified in ...


Crop -glucanase activity limits the effectiveness of a recombinant cellulase us...

Lordelo, M.M.; Ponte, P.I.P.; Guerreiro, C.I.P.D.; Soares, M.C.; Mourão, J.L.; Crespo, J.P.; Crespo, D.G.; Prates, J.A.M.; Ferreira, L.M.A.

1. The supplementation of diets rich in soluble polysaccharides with microbial cellulases and hemicellulases decreases digesta viscosity and promotes broiler performance. 2. In contrast, recent experiments suggest that polysaccharidases are ineffective for improving the nutritive value of pasture biomass used by free-range broilers. However, the feasibility of using cellulases and hemicellulases to improve the ...


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