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NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-der...
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Correia, Marlene; Ferreira, Filipa; Bonifazio, Giulia; Goodfellow, Brian J.; Oliveira, Mariana B.This Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics...
Endo- and exometabolome crosstalk in mesenchymal stem cells undergoing osteogen...
Bispo, Daniela S. C.; Michálková, Lenka; Correia, Marlene; Jesus, Catarina S. H.; Duarte, Iola F.; Goodfellow, Brian J.; Oliveira, Mariana B.This paper describes, for the first time to our knowledge, a lipidome and exometabolome characterization of osteogenic differentiation for human adipose tissue stem cells (hAMSCs) using nuclear magnetic resonance (NMR) spectroscopy. The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including ω-6 and ω-3 fatty acids), several phospholipids (phosph...
An intracellular metabolic signature as a potential donor-independent marker of...
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Romek, Katarzyna; Marques, Inês M. C.; Oliveira, Mariana B.; Mano, João F.; Gil, Ana M.This paper describes an untargeted NMR metabolomics study to identify potential intracellular donor-dependent and donor-independent metabolic markers of proliferation and osteogenic differentiation of human adipose mesenchymal stem cells (hAMSCs). The hAMSCs of two donors with distinct proliferating/osteogenic characteristics were fully characterized regarding their polar endometabolome during proliferation and...
Metabolomic applications in stem cell research: a review
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Marques, Inês M. C.; Romek, Katarzyna M.; Oliveira, Mariana B.; Mano, João F.; Gil, Ana M.This review describes the use of metabolomics to study stem cell (SC) characteristics and function, excluding SCs in cancer research, suited to a fully dedicated text. The interest in employing metabolomics in SC research has consistently grown and emphasis is, here, given to developments reported in the past five years. This text informs on the existing methodologies and their complementarity regarding the inf...
A New Folding Kinetic Mechanism for Human Transthyretin and the Influence of th...
Jesus, Catarina S. H.; Almeida, Zaida L.; Vaz, Daniela C.; Faria, Tiago Q.; Brito, Rui M. M.Protein aggregation into insoluble amyloid fibrils is the hallmark of several neurodegenerative diseases, chief among them Alzheimer's and Parkinson's. Although caused by different proteins, these pathologies share some basic molecular mechanisms with familial amyloidotic polyneuropathy (FAP), a rare hereditary neuropathy caused by amyloid formation and deposition by transthyretin (TTR) in the peripheral and au...
A New Folding Kinetic Mechanism for Human Transthyretin and the Influence of th...
Jesus, Catarina S. H.; Almeida, Zaida L.; Vaz, Daniela C.; Faria, Tiago Q.; Brito, Rui M. M.Protein aggregation into insoluble amyloid fibrils is the hallmark of several neurodegenerative diseases, chief among them Alzheimer’s and Parkinson’s. Although caused by different proteins, these pathologies share some basic molecular mechanisms with familial amyloidotic polyneuropathy (FAP), a rare hereditary neuropathy caused by amyloid formation and deposition by transthyretin (TTR) in the peripheral and au...
Changes in channel trafficking and protein stability caused by LQT2 mutations i...
Harley, Carol A.; Jesus, Catarina S. H.; Carvalho, Ricardo; Brito, Rui M. M.; Morais-Cabral, João H.Inherited human long-QT2 syndrome (LQTS) results from mutations in the gene encoding the HERG channel. Several LQT2-associated mutations have been mapped to the amino terminal cytoplasmic Per-Arnt-Sim (PAS) domain of the HERG1a channel subunit. Here we have characterized the trafficking properties of some LQT2-associated PAS domain mutants and analyzed rescue of the trafficking mutants by low temperature (27°C)...
The V30M Amyloidogenic Mutation Decreases the Rate of Refolding Kinetics of the...
Jesus, Catarina S. H.; Vaz, Daniela C.; Saraiva, Maria J. M.; Brito, Rui M. M.Transthyretin (TTR) is a homotetrameric protein implicated in several amyloid diseases. The mechanism by which TTR is converted into elongated fibrillar assemblies has been extensively investigated, and numerous studies showed that dissociation of the native tetrameric structure into partially unfolded monomeric species precedes amyloid formation. The small differences observed in the crystal structures of diff...
The V30M amyloidogenic mutation decreases the rate of refolding kinetics of the...
Jesus, Catarina S. H.; Vaz, Daniela C.; Saraiva, Maria J. M.; Brito, Rui M. M.Transthyretin (TTR) is a homotetrameric protein implicated in several amyloid diseases. The mechanism by which TTR is converted into elongated fibrillar assemblies has been extensively investigated, and numerous studies showed that dissociation of the native tetrameric structure into partially unfolded monomeric species precedes amyloid formation. The small differences observed in the crystal structures of diff...