10 documents found, page 1 of 1
Localized cancer photodynamic therapy approach based on core–shell electrospun ...
Costa, Sofia M.; Lourenço, Leandro M.O.; Calhelha, Ricardo C.; Calejo, Isabel; Barrias, Cristina C.; Fangueiro, Raul; Ferreira, Diana P.Photodynamic therapy (PDT) has been considered a promising treatment for several types of cancer, including cervical cancer. Localized drug delivery systems (DDSs) based on nanofibers produced by electrospinning have emerged as a powerful platform to carry and deliver photosensitizers (PSs) onto or adjacent to the tumor site, thereby promoting higher therapeutic efficacy and reducing the side effects to healthy...
Localized cancer photodynamic therapy approach based on core–shell electrospun ...
Costa, Sofia Maria Soares; Lourenço, Leandro M. O.; Calhelha, Ricardo C.; Calejo, Isabel; Barrias, Cristina C.; Fangueiro, Raúl; Ferreira, Diana P.Photodynamic therapy (PDT) has been considered a promising treatment for several types of cancer, including cervical cancer. Localized drug delivery systems (DDSs) based on nanofibers produced by electrospinning have emerged as a powerful platform to carry and deliver photosensitizers (PSs) onto or adjacent to the tumor site, thereby promoting higher therapeutic efficacy and reducing the side effects to healthy...
Therapeutic effects of platelet-derived extracellular vesicles in a bioengineer...
Graça, Ana L.; Domingues, Rui Miguel Andrade; Calejo, Isabel; Gómez-Florit, Manuel; Gomes, Manuela E.Tendon injuries represent over 30–50% of musculoskeletal disorders worldwide, yet the available therapies do not provide complete tendon repair/regeneration and full functionality restor-ing. Extracellular vesicles (EVs), membrane-enclosed nanoparticles, have emerged as the next breakthrough in tissue engineering and regenerative medicine to promote endogenous tissue regen-eration. Here, we developed a 3D human...
Texturing hierarchical tissues by gradient assembling of microengineered platel...
Calejo, Isabel; Reis, R. L.; Domingues, Rui Miguel Andrade; Gomes, Manuela E.The heterogeneity of the hierarchical tissues requires designing multipart engineered constructs as suitable tissue replacements. Herein, we propose the incorporation of platelet lysate (PL) within electrospun fiber core aiming the fabrication of functionally graded 3D scaffolds for heterotypic tissues regeneration, such as tendon-to-bone interface. First, anisotropic yarns (A-Yarns) and isotropic threads with ...
Bioengineered 3D living fibers as in vitro human tissue models of tendon physio...
Calejo, Isabel; Labrador-Rached, Claudia J.; Gomez-Florit, Manuel; Docheva, Denitsa; Reis, R. L.; Domingues, Rui Miguel Andrade; Gomes, Manuela E.Clinically relevant in vitro models of human tissue's health and disease are urgently needed for a better understanding of biological mechanisms essential for the development of novel therapies. Herein, physiological (healthy) and pathological (disease) tendon states are bioengineered by coupling the biological signaling of platelet lysate components with controlled 3D architectures of electrospun microfibers t...
A textile platform using continuous aligned and textured composite microfibers ...
Calejo, Isabel; Almeida, Raquel Carvalho Ferreira Costa; Reis, R. L.; Gomes, Manuela E.Tendon-to-bone interfaces exhibit a hierarchical multitissue transition. To replicate the progression from mineralized to nonmineralized tissue, a novel 3D fibrous scaffold is fabricated with spatial control over mineral distribution and cellular alignment. For this purpose, wet-spun continuous microfibers are produced using polycaprolactone (PCL)/ gelatin and PCL/gelatin/hydroxyapatite nano-to-microparticles (...
Mesenchymal stem cells empowering tendon regenerative therapies
Costa-Almeida, Raquel; Calejo, Isabel; Gomes, Manuela E.Tendon tissues have limited healing capacity. The incidence of tendon injuries and the unsatisfactory functional outcomes of tendon repair are driving the search for alternative therapeutic approaches envisioning tendon regeneration. Cellular therapies aim at delivering adequate, regeneration-competent cell types to the injured tendon and toward ultimately promoting its reconstruction and recovery of functional...
Crosstalk between adipose stem cells and tendon cells reveals a temporal regula...
Almeida, Raquel Costa; Calejo, Isabel; Reis, R. L.; Gomes, Manuela E.Tendon injuries constitute an unmet clinical challenge owing to the limited intrinsic regenerative ability of this tissue. Cell-based therapies aim at improving tendon healing through the delicate orchestration of tissue rebuilding and regain of function. Hence, human adipose-derived stem cells (hASCs) have been proposed as a promising cell source for boosting tendon regeneration. In this work, we investigated ...
Optimisation and validation of a HS-SPME–GC–IT/MS method for analysis of carbon...
Calejo, Isabel; Moreira, Nathalie; Araújo, Ana Margarida; Carvalho, Márcia; Bastos, Maria de Lourdes; Guedes de Pinho, PaulaA new and simple analytical approach consisting of an automated headspace solid-phase microextraction (HS-SPME) sampler coupled to gas chromatography-ion trap/mass spectrometry detection (GC-IT/MS) with a prior derivatization step with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) was developed to detect volatile carbonyl metabolites with low molecular weights in human urine. A central comp...
Optimisation and validation of a HS-SPME-GC-IT/MS method for analysis of carbon...
Calejo, Isabel; Moreira, Nathalie; Araújo, Ana Margarida; Carvalho, Márcia; Bastos, Maria de Lourdes; Pinho, Paula Guedes deA new and simple analytical approach consisting of an automated headspace solid-phase microextraction (HS-SPME) sampler coupled to gas chromatography-ion trap/mass spectrometry detection (GC–IT/MS) with a prior derivatization step with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) was developed to detect volatile carbonyl metabolites with low molecular weights in human urine. A central comp...