6 documents found, page 1 of 1
Transcription and splicing dynamics during early Drosophila development
Prudêncio, Pedro; Savisaar, Rosina; Rebelo, Kenny; Martinho, Rui Goncalo; Carmo-Fonseca, MariaWidespread cotranscriptional splicing has been demonstrated from yeast to human. However, most studies to date addressing the kinetics of splicing relative to transcription used either Saccharomyces cerevisiae or metazoan cultured cell lines. Here, we adapted native elongating transcript sequencing technology (NET-seq) to measure cotranscriptional splicing dynamics during the early developmental stages of Droso...
Analysis of Mammalian Native Elongating Transcript sequencing (mNET-seq) high-t...
Prudêncio, Pedro; Rebelo, Kenny; Grosso, Ana Rita; Martinho, Rui Gonçalo; Carmo-Fonseca, MariaMammalian Native Elongating Transcript sequencing (mNET-seq) is a recently developed technique that generates genome-wide profiles of nascent transcripts associated with RNA polymerase II (Pol II) elongation complexes. The ternary transcription complexes formed by Pol II, DNA template and nascent RNA are first isolated, without crosslinking, by immunoprecipitation with antibodies that specifically recognize the...
NineTeen Complex-subunit Salsa is required for efficient splicing of a subset o...
Rathore, Om Singh; Silva, Rui D; Ascensão-Ferreira, Mariana; Matos, Ricardo; Carvalho, Célia; Marques, Bruno; Tiago, Margarida N.; Prudêncio, PedroThe NineTeen Complex (NTC), also known as pre-mRNA-processing factor 19 (Prp19) complex, regulates distinct spliceosome conformational changes necessary for splicing. During Drosophila midblastula transition, splicing is particularly sensitive to mutations in NTC-subunit Fandango, which suggests differential requirements of NTC during development. We show that NTC-subunit Salsa, the Drosophila ortholog of human...
Analysis of mammalian native elongating transcript sequencing (mNET-seq) high-t...
Prudêncio, Pedro; Rebelo, Kenny; Grosso, Ana Rita; Goncalo Martinho, Rui; Carmo-Fonseca, MariaMammalian Native Elongating Transcript sequencing (mNET-seq) is a recently developed technique that generates genome-wide profiles of nascent transcripts associated with RNA polymerase II (Pol II) elongation complexes. The ternary transcription complexes formed by Pol II, DNA template and nascent RNA are first isolated, without crosslinking, by immunoprecipitation with antibodies that specifically recognize the...
Early programming of the oocyte epigenome temporally controls late prophase I t...
Navarro-Costa, Paulo; McCarthy, Alicia; Prudêncio, Pedro; Greer, Christina; Guilgur, Leonardo G.; Becker, Jörg D.; Secombe, Julie; Rangan, PrashanthOocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here w...
Requirement for highly efficient pre-mRNA splicing duringDrosophilaearly embryo...
Guilgur, Leonardo Gastón; Prudêncio, Pedro; Sobral, Daniel; Liszekova, Denisa; Rosa, André; Martinho, Rui GonçaloDrosophila syncytial nuclear divisions limit transcription unit size of early zygotic genes. As mitosis inhibits not only transcription, but also pre-mRNA splicing, we reasoned that constraints on splicing were likely to exist in the early embryo, being splicing avoidance a possible explanation why most early zygotic genes are intronless. We isolated two mutant alleles for a subunit of the NTC/Prp19 complexes, ...