10 documents found, page 1 of 1

Characterization of gastric cells infection by diverse Helicobacter pylori stra...

The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning t...

Development of synthetic light-chain antibodies as novel and potent HIV fusion ...

"Objective: To develop a novel and potent fusion inhibitor of HIV infection based on a rational strategy for synthetic antibody library construction. Design: The reduced molecular weight of single-domain antibodies (sdAbs) allows targeting of cryptic epitopes, the most conserved and critical ones in the context of HIV entry. Heavy-chain sdAbs from camelids are particularly suited for this type of epitope recogn...

Development of synthetic light-chain antibodies as novel and potent HIV fusion ...

OBJECTIVE: To develop a novel and potent fusion inhibitor of HIV infection based on a rational strategy for synthetic antibody library construction. DESIGN: The reduced molecular weight of single-domain antibodies (sdAbs) allows targeting of cryptic epitopes, the most conserved and critical ones in the context of HIV entry. Heavy-chain sdAbs from camelids are particularly suited for this type of epitope recogni...

A comprehensive high-throughput FTIR spectroscopy-based method for evaluating t...

Reporter genes are routinely used in every laboratory for molecular and cellular biology for studying heterologous gene expression and general cellular biological mechanisms, such as transfection processes. Although well characterized and broadly implemented, reporter genes present serious limitations, either by involving time-consuming procedures or by presenting possible side effects on the expression of the ...

Crenças e atitudes dos profissionais face ao abuso sexual. Estudo com polícias

Modelling, monitoring and control of plasmid bioproduction in Escherichia coli ...

An integrated approach for modelling, monitoring and control the plasmid bioproduction in Escherichia coli cultures is presented. In a first stage, by the implementation of a kinetic model for E. coli cultures, a better bioprocess understanding was reached, concerning the availability of nutrients and products along the bioprocess, and their effects on the plasmid production. Results presented may provide signi...

The importance of Helicobacter pylori's genetic variability for the constructio...

Helicobacter pylori is responsible for several gastric diseases. The main constraints of vaccine trials against this pathogen are mainly due to the bacterium high antigenic variability and to down-regulation of the host immune responses. To counteract these factors we propose a DNA vaccine able to induce a balanced humoural and citotoxic specific immune responses, based on multi-antigens. The selection of the a...

O desenvolvimento das competências transversais nos estudantes do ensino superi...

Psychometric properties of the students career concerns inventory: 1st. and 2nd...

Opportunities to strengthen the link between higher education institutions and ...