12 documents found, page 1 of 2
Structure and Absolute Configuration of Secondary Metabolites from Two Strains ...
Ortega,Humberto E.; Batista Jr.,João M.; Melo,Weilan G. P.; Paula,Gabriela T. de; Pupo,Mônica T.The antibiotic streptazolin (1), its E-isomer (2), along with the stereoisomers strepchazolin A (3) and strepchazolin B (4) and the inorganic compound cyclooctasulfur (5) were produced in solid culture by Streptomyces chartreusis ICBG377, which was isolated from the fungal garden of the leaf-cutter ant Acromyrmex subterraneus brunneus. This is the first time compound 2 is reported as a natural product. Compound...
Microbial Symbionts of Insects are the Focus of the First International Coopera...
Pupo,Mônica T.; Currie,Cameron R.; Clardy,JonThe basic aim of the ICBG (International Cooperative Biodiversity Group) program is to benefit both the host country and the global scientific community by discovering and developing new solutions to human health problems based on previously unexplored genetic resources. The first ICBG in Brazil is jointly supported by the Fogarty International Center of the National Institutes of Health (FIC/NIH) in the USA an...
ASYMMETRIC SULFOXIDATION OF ALBENDAZOLE TO RICOBENDAZOLE BY FUNGI: EFFECT OF pH
Barth,Thiago; Hilário,Viviane C.; Rocha,Bruno A.; Furtado,Niege A. J. C.; Pupo,Mônica T.; Oliveira,Anderson R. M. deAlbendazole (ABZ) is an anthelmintic drug used for the treatment of infectious diseases in veterinary and human medicine. This drug is a prochiral drug that after administration, is rapidly oxidized in the pharmacologically active sulfoxide metabolite, which is also known as ricobendazole (ABZSOX). ABZSOX has a stereogenic center and possibly two enantiomers, (+)-ABZSOX and (-)-ABZSOX. In the present work, we i...
Coupling DLLME-CE for the Stereoselective Analysis of Venlafaxine and Its Main ...
Bortoleto,Marcela A.; Bocato,Mariana Z.; Pupo,Mônica T.; Gaitani,Cristiane M.; Oliveira,Anderson R. M.Fungal biotransformations have become very important in the study of chiral drugs because the reactions performed by these microorganisms may be enantioselective. However, analyses of analytes present in liquid culture medium have proved to be very difficult due to the complexity of this matrix. The aim of this work was to couple dispersive liquid-liquid microextraction (DLLME) with capillary electrophoresis to...
Bioactive extracts and chemical constituents of two endophytic strains of Fusar...
Nascimento,Andréa M. do; Conti,Raphael; Turatti,Izabel C. C.; Cavalcanti,Bruno C.; Costa-Lotufo,Letícia V.; Pessoa,Cláudia; Moraes,Manoel O. deEthyl acetate extracts of cultures grown in liquid Czapek and on solid rice media of the fungal endophyte Fusarium oxysporum SS46 isolated from the medicinal plant Smallanthus sonchifolius (Poepp.) H. Rob., Asteraceae, exhibited considerable cytotoxic activity when tested in vitro against human cancer cells. Chromatographic separation yielded anhydrofusarubin (1) and beauvericin (2) that were identified based o...
Biosynthesis of aphidicolin proceeds via the mevalonate pathway in the endophyt...
Lopes,Adriana A.; Pupo,Mônica T.We have previously identified the endophytic fungus Nigrospora sphaerica as a prolific producer of the bioactive diterpene aphidicolin. Herein we report a study to establish the best conditions for the production of aphidicolin by N. sphaerica in Czapek medium. The sugar source (glucose and sucrose) and the incubation time (4-15 days) were optimized for further application on biosynthetic studies of the diterpe...
Fast HPLC analysis of omeprazole, 5-hydroxyomeprazole and omeprazole sulfone in...
Borges,Keyller B.; Durán-Patrón,Rosa; Sánchez,Antonio José M.; Pupo,Mônica T.; Bonato,Pierina S.; Collado,Isidro G.A fast liquid chromatography method was developed and validated for the simultaneous determination of omeprazole (OMZ), 5-hydroxyomeprazole (5-HOMZ) and omeprazole sulphone (OMZ SUL) in liquid culture medium for application in biotransformation studies employing phytopathogenic and endophytic fungi. The separation was achieved using a monolithic Chromolith Fast gradient RP 18 endcapped column, using a mobile ph...
A simple method for the quantitative analysis of tyrosol by hplc in liquid Czap...
Guimarães,Denise O.; Borges,Keyller B.; Bonato,Pierina S.; Pupo,Mônica T.Tyrosol is a possible quorum sensing molecule in endophytic fungi. High-performance liquid chromatography (HPLC) coupled with diode array detector (DAD) was used for the analysis of tyrosol in liquid Czapek fungal cultures. The optimized conditions were gradient mobile phase, in linear mode, consisting initially of acetonitrile/water (1:9 v/v) and increasing up to acetonitrile (100%) in 30 minutes at a flow rat...
Biotransformation of a tetrahydrofuran lignan by the endophytic fungus Phomopsi...
Verza,Michelle; Arakawa,Nilton S.; Lopes,Norberto P.; Kato,Massuo J.; Pupo,Mônica T.; Said,Suraia; Carvalho,IvoneThe biotrasformation of the tetrahydrofuran lignan, (-)-grandisin, by the endophitic fungus Phomopsis sp, obtained from Viguiera arenaria, led to the formation of a new compound determined as 3,4-dimethyl-2-(4'-hydroxy-3',5'-dimethoxyphenyl)-5-methoxy-tetrahydrofuran. The metabolite was evaluated against the parasite Trypanosoma cruzi, the causative agent of Chagas's disease, and showed a trypanocidal activity ...
Chaetoglobosinas produzidas por Chaetomium globosum, fungo endofítico associado...
Momesso,Luciano da S.; Kawano,Cristina Y.; Ribeiro,Patrícia H.; Nomizo,Auro; Goldman,Gustavo H.; Pupo,Mônica T.Endophytes live in association with host plants during all or part of their life cycle without causing any apparent disease. They are considered outstanding and underexploited sources of novel bioactive compounds. Chaetomium globosum was isolated as an endophytic fungus from the healthy leaves of Viguiera robusta. C.globosum is a remarkable producer of chaetoglobosins, which are typically cytotoxic. In this wor...