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Atividade antitrombótica dos polissacarídeos sulfatados da alga marinha vermelh...
Queiroz, Ismael Nilo Lino deThe interest in the isolation of sulfated polysaccharides from seaweed with anticoagulant and antithrombotic activities are justified by events of thrombosis, the adverse effects of heparin, a widely used drug in the prevention and treatment of thromboembolic diseases. Adding to that sulfated polysaccharides (PS) has attracted a wide interest in various fields of medical sciences. The aim of this study was to e...
Structural and physical-chemical analyses of sulfated polysaccharides from the ...
Barcellos, Priscila Gomes; Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Araújo, Ianna Wivianne Fernandes deUlva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physical-chemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, gluco...
Caracterização estrutural preliminar e efeitos na inflamação da lectina da alga...
Queiroz, Ismael Nilo Lino deLectins seaweed have various pharmacological applications. This work was partially characterize the structure and evaluate the effects on classical models of inflammation lectin of the green seaweed Caulerpa cupressoides. The Caulerpa cupressoides lectin (CcL) was extracted with Tris-HCl 25 mM, pH 7.5 and isolated by ion exchange chromatography on DEAE-cellulose. Structural characterization showed a partial ami...
Mild-acid hydrolysis of a native polysulfated fraction from Acanthophora muscoi...
Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria Barros; Tovar, Ana Maria FreireAcanthophora muscoides (Rhodophyta) has three polysulfated fractions (Am-1, Am-2 and Am-3). Am-2 displayed anti-inflammation and serpin-independent anticoagulation; however, no effect of oligomers on thrombin-generation (TG) assay has been demonstrated. This study employed mild-acid hydrolysis to obtain low-molecular-size derivatives from Am-2 and compared in vitro inhibitory effects between intact Am-2 and ...
Extraction and structural properties of Acanthophora muscoides (Rhodophyceae) e...
Rodrigues, José Ariévilo Gurgel; Queiroz, Ismael Nilo Lino de; Quinderé, Ana Luíza Gomes; Benevides, Norma Maria Barros; Tovar, Ana Maria FreireAcanthophora muscoides (Rhodophyta) contains structurally heterogeneous sulfated polysaccharides (Am-SPs) with pharmacological importance; however, its matrix SPs composition has not been still extensively investigated. This study sequentially extracted and compared the structural features and the in vitro anticoagulant effects of the Am-SPs. Papain-extraction sequence yielded Am.E-1, Am.E-2 and Am.E-3 containi...
Analysis of two precipitation methods on the yield, structural features and act...
Souza, Ricardo Basto; Queiroz, Ismael Nilo Lino de; Silva Neto, Antônio Alves da; Carneiro, José Gerardo; Rodrigues, José Ariévilo GurgelThe global demand for natural products from seaweeds has increased worldwide; however, no description of the use of isoamly alcohol (IAA) for obtaining of sulfated polysaccharides (SPs) has been reported. We investigated the efficiency of two precipitation methods (M) in obtaining SPs from the red seaweed Gracilaria cornea. SPs enzymatically isolated were concentrated with cetylpyridinium chloride (M I) or IAA ...
Peripheral antinociception and anti-edematogenic effect of a sulfated polysacch...
Quinderé, Ana L. G.; Fontes, Bruno P.; Vanderlei, Edfranck de S. O.; Rodrigues, José A. G.; Araújo, Ianna W. F. de; Jorge, Roberta J. B.Background: Sulfated polysaccharides from red marine algae have presented a variety of potentially therapeutic biological effects, however, their antinocicpetive and anti-inflammatory properties are not well understood. Methods: Male Swiss mice were pretreated with a sulfated polysaccharidic fraction obtained from the marine alga Acanthophora muscoides (AmII) (1, 3 or 9 mg/kg, iv) 30 min prior to either receivi...
Inhibition of coagulation proteases and thrombosis and sub-chronic toxicologica...
Queiroz, Ismael Nilo Lino de; Rodrigues, José Ariévilo Gurgel; Quinderé, Ana Luíza Gomes; Holanda, Márjory Lima; Pereira, Maria GonçalvesMetabolites isolated from Gelidiella species (Rhodophyta) have been few studied. We evaluated a sulfated polysaccharidic fraction from G. acerosa collected from two Brazilian beaches on the northwestern coast of Brazil (Flecheiras-F and Pedra Rachada-PR) on coagulation proteases and thrombosis. Their toxicity in vivo was also assessed. Enzymatic extractions yielded 1.40%, and similar chromatographic profiles (D...
Analysis of two drying methods on the yield and activity of sulfated polysaccha...
Rodrigues, José Ariévilo Gurgel; Vanderlei, Edfranck de Sousa Oliveira; Quinderé, Ana Luíza Gomes; Queiroz, Ismael Nilo Lino de; Bessa, Érika FaçanhaSulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from Halymenia sp Rhodophyceae in order to compare the yield, purification and anticoagulant activity. The total SPs (TSPs) were first extracted with papain in 100 mM sodium acetate (pH 5.0) containing cysteine and EDTA (5 mM). ...