7 documents found, page 1 of 1
Effective separation of nucleic acids from complex bacterial lysates using thre...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniNo abstract available.
Purification of RNA from recombinant lysates using biocompatible amino-acid-bas...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniRNA-based biopharmaceuticals are being envisioned as a powerful tool for the development of innovative medicines for prevalent diseases. Despite their undeniable relevance, the ubiquitous unstable nature of RNA coupled with the laborious and costly methods required for its extraction and purification still challenge the widespread application of this biopolymer. On the basis of the high affinity between amino-a...
Aqueous biphasic systems composed by biocompatible amino acid-based ionic liqui...
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniThe emergence of RNA as a promising biopharmaceutical paved the way for the development of innovative medicines with broad therapeutic and prophylactic efficiencies in infectious diseases and cancer. Notwithstanding their relevance on this field, the labile nature and intrinsic low stability of RNA coupled with the laborious and costly methods of extraction and purification of this biopolymer constitute the mai...
Integrated extraction-preservation of bacterial RNAs using ionic liquids
Quental, Maria J.; Pedro, Augusto Q.; Pereira, Patrícia; Mukesh; Coutinho, João A. P.; Sousa, Fani; Freire, Mara G.Ribonucleic acid (RNA) is a biopolymer of high relevance in the biopharmaceuticals field and in fundamental and applied research. In all these applications, the methods and solvents used for extracting and purifying RNA from various types of cells or other biological matrices, and respective preservation, are critical issues (Tateishi-Karimata & Sugimoto, 2018). Considering that RNA is a highly labile polymer w...
Stabilization and purification of RNA using biocompatible ionic liquids
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniThe widespread use of RNA for the development of novel therapies has been hindered due to issues associated with its inherent chemical instability and fast degradation by ribonucleases1. In addition, the traditional extraction and purification techniques of this biomolecule are time-consuming and require highly toxic reagents, demanding for improved technologies able to provide RNA with high integrity, purity a...
Stabilization and purification of RNA using biobased ionic liquids
Pereira, Ana F.; Pedro, Augusto Q.; Castro, Leonor S.; Quental, Maria J.; Tavares, Ana P. M.; Branco, Luís C.; Coutinho, João A. P.; Sousa, FaniThe ubiquitous unstable nature of RNA has been preventing its widespread use in the development of novel therapies. Moreover, the traditional RNA extraction and purification techniques are laborious and require highly toxic reagents, demanding for improved technologies able to provide RNA with high purity and biological activity. To overcome the described bottlenecks, ionic-liquid-based aqueous biphasic systems...
Cholinium-Based Good’s Buffers Ionic Liquids as Remarkable Stabilizers and Recy...
Pedro, Augusto; Pereira, Patrícia; Quental, Maria J.; Carvalho, André P.; Santos, Sérgio M.; Queiroz, João; Sousa, Fani; Freire, Mara G.RNA is a biopolymer of high relevance in the biopharmaceuticals field and in fundamental and applied research; however, the preservation of the RNA stability is still a remarkable challenge. Herein, we demonstrate the enhanced potential of aqueous solutions of self-buffering cholinium-based Good's buffers ionic liquids (GB-ILs), at 20 and 50 % (w/w), as alternative preservation media of recombinant small RNAs. ...