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2,4,5-Triaminopyrimidines as blue fluorescent probes for cell viability monitor...

Monitoring cell viability is critical in cell biology, pathology, and drug discovery. Most cell viability assays are cell-destructive, time-consuming, expensive, and/or hazardous. Herein, we present a series of newly synthesized 2,4,5-triaminopyrimidine derivatives able to discriminate between live and dead cells. To our knowledge, these compounds are the first fluorescent nucleobase analogues (FNAs) with cell ...

Counteracting Colon Cancer by Inhibiting Mitochondrial Respiration and Glycolys...

Metabolic reprogramming is a central hub in tumor development and progression. Therefore, several efforts have been developed to find improved therapeutic approaches targeting cancer cell metabolism. Recently, we identified the 7a-acetoxy-6b-benzoyloxy-12-O-benzoylroyleanone (Roy- Bz) as a PKC -selective activator with potent anti-proliferative activity in colon cancer by stimulating a PKC -dependent mitochondr...

Adenosinergic system and BDNF signaling changes as a cross-sectional feature of...

Rett Syndrome is an X-linked neurodevelopmental disorder (RTT; OMIM#312750) associated to MECP2 mutations. MeCP2 dysfunction is seen as one cause for the deficiencies found in brain-derived neurotrophic factor (BDNF) signaling, since BDNF is one of the genes under MeCP2 jurisdiction. BDNF signaling is also dependent on the proper function of the adenosinergic system. Indeed, both BDNF signaling and the adenosin...

Mesial Temporal Lobe Epilepsy (MTLE) Drug-Refractoriness Is Associated With P2X...

Objective: ATP-gated ionotropic P2X7 receptors (P2X7R) actively participate in epilepsy and other neurological disorders. Neocortical nerve terminals of patients with Mesial Temporal Lobe Epilepsy with Hippocampal Sclerosis (MTLE-HS) express higher P2X7R amounts. Overexpression of P2X7R bolsters ATP signals during seizures resulting in glial cell activation, cytokines production, and GABAergic rundown with unre...

Opposing Effects of Adenosine and Inosine in Human Subcutaneous Fibroblasts May...

Human subcutaneous fibroblasts (HSCF) challenged with inflammatory mediators release huge amounts of ATP, which rapidly generates adenosine. Given the nucleoside's putative relevance in wound healing, dermal fibrosis, and myofascial pain, we investigated the role of its precursor, AMP, and of its metabolite, inosine, in HSCF cells growth and collagen production. AMP (30 µM) was rapidly (t½ 3 ± 1 min) dephosphor...

Impairment of adenosinergic system in Rett syndrome: novel therapeutic target t...

Rett syndrome (RTT; OMIM#312750) is mainly caused by mutations in the X-linked MECP2 gene (methyl-CpG-binding protein 2 gene; OMIM*300005), which leads to impairments in the brain-derived neurotrophic factor (BDNF) signalling. The boost of BDNF mediated effects would be a significant breakthrough but it has been hampered by the difficulty to administer BDNF to the central nervous system. Adenosine, an endogenou...

Neuromuscular paralysis by the basic phospholipase A2 subunit of crotoxin from ...

Made available in DSpace on 2018-12-11T17:14:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-11-01; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação para a Ciência e a Tecnologia; Background and purpose Crotoxin (CTX), a heterodimeric phospholipase A2 (PLA2) neurotoxin from Crotalus durissus terrificus s...

The Use of Laser Microdissection in Forensic Sexual Assault Casework: Pros and ...

Laser scanning microdissection - Advantages and pitfalls in forensic diagnosis.

Laser microdissection (LMD) is a technology that has been around for more than 40 years. It combines the amplification power of a microscope with the precision cut of objects allowed by the laser technology. The main use of a Laser Microdissection System (LMS) is for clinical purposes, in order to isolate cells or its components of interest. Only in the last decade has LMD been used for forensic purposes, mainl...

Calcium signaling and the novel anti-proliferative effect of the UTP-sensitive ...

During myocardial ischemia and reperfusion both purines and pyrimidines are released into the extracellular milieu, thus creating a signaling wave that propagates to neighboring cells via membrane-bound P2 purinoceptors activation. Cardiac fibroblasts (CF) are important players in heart remodeling, electrophysiological changes and hemodynamic alterations following myocardial infarction. Here, we investigated th...