8 documents found, page 1 of 1
Development of conjugated kefiran-chondroitin sulphate cryogels with enhanced p...
Radhouani, Hajer; Gonçalves, Cristiana; Maia, F. Raquel; Oliveira, Maria Eduarda Moreira Pinheiro Santos; Reis, R. L.; Oliveira, Joaquim M.Hydrogels based on natural polysaccharides can have unique properties and be tailored for several applications, which may be mainly limited by the fragile structure and weak mechanical properties of this type of system. We successfully prepared cryogels made of newly synthesized kefiran exopolysaccharide-chondroitin sulfate (CS) conjugate via carbodiimide-mediated coupling to overcome these drawbacks. The freez...
Impact of kefiran exopolysaccharide extraction on its applicability for tissue ...
Correia, Susana; Gonçalves, Cristiana; Oliveira, Joaquim M.; Radhouani, Hajer; Reis, R. L.Kefiran is an exopolysaccharide produced by the microflora of kefir grains used to produce the fermented milk beverage kefir. The health-promoting and physicochemical properties of kefiran led to its exploration for a range of applications, mainly in the food industry and biomedical fields. Aiming to explore its potential for tissue engineering and regenerative medicine (TERM) applications, the kefiran biopolym...
Fabrication of biocompatible porous SAIB/silk fibroin scaffolds using ionic liq...
Gonçalves, Cristiana; Gomes, J. M.; Maia, F. Raquel; Radhouani, Hajer; Silva, S. S.; Reis, R. L.; Oliveira, J. M.Sucrose acetate isobutyrate (SAIB) is a highly viscous hydrogel and presents many challenges for processing when its application in tissue engineering is envisioned. Herein, is proposed an innovative methodology by processing SAIB with ionic liquids (ILs), combined with silk fibroin (SF), to develop SAIB/SF scaffolds. Fourier Transform Infrared analysis confirmed SF and SAIB presence in the structures and the e...
Synthesis and characterization of biocompatible methacrylated Kefiran hydrogels...
Radhouani, Hajer; Correia, Susana; Gonçalves, Cristiana; Reis, R. L.; Oliveira, J. M.Hydrogel application feasibility is still limited mainly due to their low mechanical strength and fragile nature. Therefore, several physical and chemical cross-linking modifications are being used to improve their properties. In this research, methacrylated Kefiran was synthesized by reacting Kefiran with methacrylic anhydride (MA). The developed MA-Kefiran was physicochemically characterized, and its biologic...
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the progr...
Ribeiro, Viviana P.; Silva-Correia, Joana; Gonçalves, Cristiana; Pina, Sandra; Radhouani, Hajer; Montonen, Toni; Oliveira, Joaquim M.; Oliveira, Ana L.Timely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response to conformational transitions of proteins, are of great interest in cancer research and treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-d...
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the progr...
Ribeiro, V. P.; Silva-Correia, J.; Gonçalves, C.; Pina, S.; Radhouani, Hajer; Montonen, Toni; Hyttinen, J.; Roy, A.; Oliveira, A. L.; Reis, R. L.Timely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response to conformational transitions of proteins, are of great interest in cancer research and treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-d...
Promising biomolecules
Oliveira, Isabel M.; Carvalho, Ana L.; Radhouani, Hajer; Gonçalves, Cristiana; Oliveira, J. M.; Reis, R. L.The osteochondral defect (OD) comprises the articular cartilage and its subchondral bone. The treatment of these lesions remains as one of the most problematic clinical issues, since these defects include different tissues, requiring distinct healing approaches. Among the growing applications of regenerative medicine, clinical articular cartilage repair has been used for two decades, and it is an effective exam...
Genomic and proteomic study of antimicrobial resistance in Escherichia coli and...
Radhouani, HajerAs ferramentas genómicas, proteómicas e bioinformáticas permitem um conhecimento mais aprofundado sobre a fisiologia e estrutura bacterianas e também dos mecanismos envolvidos na resistência antimicrobiana. A resistência aos antimicrobianos tem evoluído e disseminado entre bactérias, representando uma séria e crescente ameaça para a saúde pública. No entanto, esta resistência tem sido detectada também na ausênc...