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Cell-free DNA as a new biomarker of IVF success, independent of any infertility...

Casteleiro Alves, Maria Manuel; Oliani, Luísa; Almeida, Micaela; Cardoso, Henrique José; Oliani, António Hélio; Breitenfeld, Luiza

Cell-free DNA fragments detected in blood and in other biological fluids are released from apoptotic/necrotic cells. In this study, we analyzed cfDNA levels in follicular fluid (FF) samples from patients with infertility. Samples were collected from 178 infertile women and cfDNA was extracted and quantified by qPCR, using ALU115 and ALU247 primers, and statistical correlations were performed. We found that cfDN...


Determinação de potenciais biomarcadores de infertilidade feminina : estudo piloto

Brinca, Ana Teresa; Anjos, O.; Alves, Maria Manuel; Sousa, Ângela; Oliani, António Hélio; Breitenfeld, Luísa; Passarinha, Luís A.

Devido à sua elevada incidência, a infertilidade tornou-se uma questão de saúde pública proeminente, representando um desafio significativo para a medicina reprodutiva moderna.


Volatilomics as an emerging strategy to determine potential biomarkers of femal...

Brinca, Ana Teresa; Anjos, O.; Alves, Maria Manuel; Sousa, Ângela; Oliani, António Hélio; Breitenfeld, Luísa; Passarinha, Luís A.

Due to its high prevalence, infertility has become a prominent public health issue, posing a significant challenge to modern reproductive medicine. Some clinical conditions that lead to female infertility include polycystic ovary syndrome (PCOS), endometriosis, and premature ovarian failure (POF). Follicular fluid (FF) is the biological matrix that has the most contact with the oocyte and can, therefore, be use...


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