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Interaction of Hoechst 33342 with POPC Membranes at Different pH Values
Cordeiro, Margarida M.; Filipe, Hugo A. L.; Santos, Patrícia dos; Samelo, Jaime Amadeu Cruz Reis; Ramalho, João P Prates; Loura, Luís M. S.Hoechst 33342 (H33342) is a fluorescent probe that is commonly used to stain the DNA of living cells. To do so, it needs to interact with and permeate through cell membranes, despite its high overall charge at physiological pH values. In this work, we address the effect of pH in the association of H33342 with lipid bilayers using a combined experimental and computational approach. The partition of H33342 to 1-p...
Effect of amphipathic HIV fusion inhibitor peptides on POPC and POPC/cholestero...
Canto, António M T Martins do; Carvalho, Alfredo J Palace; Ramalho, João P Prates; Loura, Luís M. S.T-20 and T-1249 fusion inhibitor peptides were shown to interact with 1-palmitoyl-2-oleyl-phosphatidylcholine (POPC) (liquid disordered, ld) and POPC/cholesterol (1:1) (POPC/Chol) (liquid ordered, lo) bilayers, and they do so to different extents. Although they both possess a tryptophan-rich domain (TRD), T-20 lacks a pocket binding domain (PBD), which is present in T-1249. It has been postulated that the PBD d...