6 documents found, page 1 of 1
Inexpensive small molecules as promising fluorescent labels for biomolecules.
Eustáquio, Raquel; Ramalho, João Paulo; Caldeira, Ana Teresa; Pereira, AntónioFluorescent labels are indispensable in various modern scientific applications, including direct and indirect immunochemistry, fluorescence microscopy, histochemistry, flow cytometry and fluorescence in situ hybridization (FISH) [1,2]. Small fluorescent labels offer significant practical benefits, enabling the optimization of fluorescence signals through the attachment of multiple fluorophores to a single biomo...
Rational Design of Cost-Effective 4-Styrylcoumarin Fluorescent Derivatives for ...
Eustáquio, Raquel; Ramalho, João Paulo; Caldeira, Ana Teresa; Pereira, AntónioFluorescent labels are key tools in a wide range of modern scientific applications, such as fluorescence microscopy, flow cytometry, histochemistry, direct and indirect immunochemistry, and fluorescence in situ hybridization (FISH). Small fluorescent labels have important practical advantages as they allow maximizing the fluorescence signal by binding multiple fluorophores to a single biomolecule. At present, t...
Affordable Small Molecules as Promising Fluorescent Labels for Biomolecules
Eustáquio, Raquel; Ramalho, João Paulo; Arantes, Sílvia; Candeias, António; Caldeira, Ana Teresa; Pereira, AntónioFluorescent labels, commonly used in highly sensitive analytical techniques for detecting and tracking biomolecules in critical fields like cellular biology, medicine, medicinal chemistry, and environmental science, are currently too expensive for routine use in standard applications, with most exhibiting small Stokes shifts. This limitation underscores the potential of 4-diethylaminobenzaldehyde derivatives as...
A magnetic controllable tool for the selective enrichment of dimethoate from ol...
Garcia, Raquel; Carreiro, Elisabete; Ramalho, João Paulo; Mirão, José; Burke, Anthony; Gomes da Silva, Marco; Costa Freitas, Ana MariaAiming to develop a straightforward magnetic-based sample preparation methodology for the selective extraction of dimethoate from olive oil, the synthesis of dimethoate-imprinted polymer on the surface of modified magnetic nanoparticles has been attempted. Molecular recognition assays have proven their suitability for the selective pre-concentration of dimethoate. Mechanistic basis for template selective recogn...
A catalytic route to dibenzodiazepines involving Buchwald-Hartwig coupling: rea...
Burke, Anthony; Marques, CAROLINA; Peixoto, Daniela; Locati, Abel; Goth, Albertino; Ramalho, João PauloWe report a new synthetic method for the synthesis of a family of dibenzodiazepines (DBDAs), employing Pd-catalyzed C-N coupling of o-bromoaldimine, with o-bromoaniline as the key step. Eleven DBDAs were prepared, containing electron-withdrawing groups (CN, F, NO2) and electron-donating groups (OMe). The reaction conditions were optimized (catalyst, phosphine, base and solvent) and best results were obtained wi...
Evaluation of Phosphinoamidoester-Derived Pd Catalysts in the Asymmetric Allyli...
Burke, Anthony; Ramalho, João Paulo; Marinho, VandaTwo simple hemilabile P,O-coordinating phosphinoamidoester ligands 6a and 6b were synthesized and studied in the Pd(0)-catalyzed asymmetric allylic alkylation of rac-1,3-diphenylpropenyl acetate affording a highest ee of 83% ee with 6a. To gain an insight into the actual mechanism of this catalytic reactions, which had previously been investigated with a first generation family of P,O-coordinating phosphinoamid...