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Differential Oxygen Exposure Modulates Mesenchymal Stem Cell Metabolism and Pro...
Moniz, Inês; Ramalho-Santos, João; Branco, Ana F.Mesenchymal stem cells reside under precise hypoxic conditions that are paramount in determining cell fate and behavior (metabolism, proliferation, differentiation, etc.). In this work, we show that different oxygen tensions promote a distinct proliferative response and affect the biosynthetic demand and global metabolic profile of umbilical cord-mesenchymal stem cells (UC-MSCs). Using both gas-based strategies...
Mushrooms on the plate: Trends towards NAFLD treatment, health improvement and ...
Fontes, Adriana; Ramalho-Santos, João; Zischka, Hans; Azul, Anabela Marisa de Jesus RodriguesNon-alcoholic fatty liver disease (NAFLD) is a most important cause of liver disease. Similar to other non-communicable diseases (NCD), such as obesity and type II diabetes mellitus, NAFLD can strongly affected by diet. Diet-related NCD and malnutrition are rising in all regions being a major cause of the global health, economic and environmental burdens. Mushrooms, important dietary components since the hunter...
Using Data Mining to Assist in Predicting Reproductive Outcomes Following Varic...
Sousa, Ana Paula; Santos-Pereira, Judith; Freire, Maria José; Parada, Belmiro; Almeida Santos, Teresa; Bernardino, Jorge; Ramalho-Santos, JoãoWe carried out a retrospective analysis of infertile couple data using several methodologies and data analysis techniques, including the application of a novel data mining approach for analyzing varicocele treatment outcomes. The aim of this work was to characterize embolized varicocele patients by ascertaining the improvement of some of their clinical features, predicting the success of treatment via pregnancy...
Fluorescent probes for the detection of reactive oxygen species in human sperma...
Escada-Rebelo, Sara; Mora, Francisca; Sousa, Ana; Almeida Santos, Teresa; Paiva, Artur; Ramalho-Santos, JoãoReactive oxygen species (ROS) production is a by-product of mitochondrial activity and is necessary for the acquisition of the capacitated state, a requirement for functional spermatozoa. However, an increase in oxidative stress, due to an abnormal production of ROS, has been shown to be related to loss of sperm function, highlighting the importance of an accurate detection of sperm ROS, given the specific natu...
Effects of DMSO on the Pluripotency of Cultured Mouse Embryonic Stem Cells (mESCs)
Sousa, Maria Inês; Correia, Bibiana; Branco, Ana Filipa; Rodrigues, Ana Sofia; Ramalho-Santos, JoãoDMSO is a commonly used solvent in biological studies, as it is an amphipathic molecule soluble in both aqueous and organic media. For that reason, it is the vehicle of choice for several water-insoluble substances used in research. At the molecular and cellular level, DMSO is a hydrogen-bound disrupter, an intercellular electrical uncoupler, and a cryoprotectant, among other properties. Importantly, DMSO often...
Antioxidant Versus Pro-Apoptotic Effects of Mushroom-Enriched Diets on Mitochon...
Fontes, Adriana; Alemany-Pagès, Mireia; Oliveira, Paulo J.; Ramalho-Santos, João; Zischka, Hans; Azul, Anabela MarisaMitochondria play a central role in non-alcoholic fatty liver disease (NAFLD) progression and in the control of cell death signalling during the progression to hepatocellular carcinoma (HCC). Associated with the metabolic syndrome, NAFLD is mostly driven by insulin-resistant white adipose tissue lipolysis that results in an increased hepatic fatty acid influx and the ectopic accumulation of fat in the liver. Up...
Bridging Health and Social Care with the Citizens – The Case of EIT Health Proj...
Sá-Moura, Bebiana; Couceiro, Patrícia; Catarino, Luis; Guardado, Diana; Brito, Maja de; Silva, Bárbara; Tavares, Rui; Ramalho-Santos, JoãoThe global burden of population ageing imposes the need of innovative care models and good practices to ensure the sustainability of health and social care support, as well as optimal quality of life of the citizens. Science and technology-based innovation is required to be cross-fertilized with co-creation strategies, involving different stakeholders and the citizens. Health literacy is a crucial component of ...
Can we induce spermatogenesis in the domestic cat using an in vitro tissue cult...
Silva, Andreia F.; Escada-Rebelo, Sara; Amaral, Sandra; Santos Tavares, Renata; Schlatt, Stefan; Ramalho-Santos, João; Mota, Paula C.The reduced number of animals in most wild felid populations implies a loss of genetic diversity. The death of juveniles, prior to the production of mature sperm, represents a loss of potential genetic contribution to future populations. Since 2011 mouse testicular organ culture has introduced an alternative mechanism to produce sperm in vitro from immature tissue. However, extension of this technology to other...
(The Symbiosis Tellus) Communicating Science through Comics
Tavares, Rui; Ramalho-Santos, João; Jorge, Susana; Azul, Anabela MarisaLife on Earth is sustained by symbiotic interactions, beginning about 460 million years ago. In parallel, we are living some of the most rapid changes in both nature and climate changes in the world’s history. These nature/climate events are intimately related with human activities; it is particularly urgent to convey the biologic basics of the beneficial symbiosis that link individuals for multi-species networ...
Pluri-IQ: Quantification of Embryonic Stem Cell Pluripotency through an Image-B...
Perestrelo, Tânia; Chen, Weitong; Correia, Marcelo; Le, Christopher; Pereira, Sandro; Rodrigues, Ana S.; Sousa, Maria I.; Ramalho-Santos, JoãoImage-based assays, such as alkaline phosphatase staining or immunocytochemistry for pluripotent markers, are common methods used in the stem cell field to assess pluripotency. Although an increased number of image-analysis approaches have been described, there is still a lack of software availability to automatically quantify pluripotency in large images after pluripotency staining. To address this need, we de...