3 documents found, page 1 of 1
High-efficiency stem cell fusion-mediated assay reveals Sall4 as an enhancer of...
Wong, Connie C.; Maia, Alexandre Gaspar; Ramalho-Santos, Miguel; Reijo Pera, Renee A.Several methods allow reprogramming of differentiated somatic cells to embryonic stem cell-like cells. However, the process of reprogramming remains inefficient and the underlying molecular mechanisms are poorly understood. Here, we report the optimization of somatic cell fusion with embryonic stem cells in order to provide an efficient, quantitative assay to screen for factors that facilitate reprogramming. Fo...
Systematic identification of cis-regulatory sequences active in mouse and human...
Grskovic, Marica; Chaivorapol, Christina; Gaspar-Maia, Alexandre; Li, Hao; Ramalho-Santos, MiguelUnderstanding the transcriptional regulation of pluripotent cells is of fundamental interest and will greatly inform efforts aimed at directing differentiation of embryonic stem (ES) cells or reprogramming somatic cells. We first analyzed the transcriptional profiles of mouse ES cells and primordial germ cells and identified genes upregulated in pluripotent cells both in vitro and in vivo. These genes are enric...
Identification and proteolytic processing of procardosin A
Ramalho-Santos, Miguel; Veríssimo, Paula; Cortes, Luísa; Samyn, Bart; Beeumen, Jozef Van; Pires, Euclides; Faro, CarlosPlant aspartic proteinases contain a plant-specific insert (PSI) of about 100 amino acids of unknown function with no similarity with the other aspartic proteinases but with significant similarity with saposins, animal sphingolipid activator proteins. PSI has remained elusive at the protein level, suggesting that it may be removed during processing. To understand the molecular relevance of PSI, the proteolytic ...