34 documents found, page 1 of 4
Sustained importance of Streptococcus pneumoniae among pediatric complicated Pn...
Gomes-Silva, J.; Silva-Costa, Catarina; Pinho, Marcos; Friães, Ana; Ramirez, Mário; Melo-Cristino, JoséBackgrounds: To improve the etiological diagnosis of culture-negative pediatric complicated pneumonia (PCP), we expanded our real-time PCR assay to include other bacterial agents and evaluate potential changes in etiology after 7 years of near universal use of 13-valent conjugate pneumococcal vaccine (PCV13). Methods: We collected 156 culture-negative pleural fluid and empyema samples from children (<18 years),...
Sustained increase of paediatric invasive Streptococcus pyogenes infections dom...
Gouveia, Catarina; Bajanca-Lavado, Maria Paula; Mamede, Rafael; Araújo Carvalho, Ana; Rodrigues, Fernanda; Cristino, José Melo; Ramirez, MárioSince autumn 2022, observed numbers of paediatric invasive group A Streptococcus infections in Portugal (n = 89) were higher than in pre-COVID-19 seasons. Between September 2022 and May 2023, the dominant diagnoses were pneumonia (25/79), mostly with empyema (20/25), and sepsis (22/79). A number of cases required admission to intensive care (27/79) and surgery (35/79), and the case fatality rate was 5.1% (4/79)...
Continued vaccine breakthrough cases of serotype 3 complicated pneumonia in vac...
Silva-Costa, Catarina; Silva, Joana; Pinho, Marcos Daniel; Friães, Ana; Ramirez, Mário; Cristino, José Melo; Oliveira, Laura Andrade deWe previously reported that despite the use of pneumococcal conjugate vaccines (PCVs), vaccine serotypes remained important causes of pneumonia with pleural effusion and empyema (pediatric complicated pneumococcal pneumonia [PCPP]). We cultured and performed PCR on 174 pleural fluid samples recovered from pediatric patients in Portugal from 2016 to 2019 to identify and serotype Streptococcus pneumoniae. Most PC...
Characteristics of Streptococcus agalactiae colonizing nonpregnant adults suppo...
Martins, Elisabete R.; Nascimento do Ó, Dulce; Marques Costa, Ana Luísa; Cristino, José Melo; Ramirez, MárioThe prevalence and lineages of Streptococcus agalactiae (group B streptococci [GBS]) colonizing pregnant women are well studied, but less is known about colonization of nonpregnant adults. We characterized GBS colonization in adults as a potential reservoir for infections and tested for the presence of clones with a potentially higher invasive disease potential. We evaluated GBS gastrointestinal, genitourinary,...
Pediatric invasive Pneumococcal disease three years after PCV13 introduction in...
Silva-Costa, Catarina; Silva, Joana; Prados, Lúcia; Ramirez, Mário; Melo Cristino, JoséThe introduction of pneumococcal conjugate vaccines PCV7 and PCV13 led to decreases in incidence of pediatric invasive pneumococcal disease (pIPD) and changes in serotype distribution. We evaluated the consequences of higher vaccine uptake after the introduction of PCV13 in the National Immunization Plan (NIP) in 2015. Besides culture and conventional serotyping, the use of molecular methods to detect and serot...
DEN-IM: dengue virus genotyping from amplicon and shotgun metagenomic sequencing
Mendes, Catarina Inês; Lizarazo, Erley; Machado, Miguel P.; Silva, Diogo N.; Tami, Adriana; Ramirez, Mário; Couto, Natacha; Rossen, John W. A.Dengue virus (DENV) represents a public health threat and economic burden in affected countries. The availability of genomic data is key to understanding viral evolution and dynamics, supporting improved control strategies. Currently, the use of high-throughput sequencing (HTS) technologies, which can be applied both directly to patient samples (shotgun metagenomics) and to PCR-amplified viral sequences (amplic...
Seroprevalence of anti‐SARS‐CoV‐2 antibodies in COVID‐19 patients and healthy v...
Figueiredo-Campos, Patricia; Blankenhaus, Birte; Mota, Catarina; Gomes, Andreia; Serrano, Marta; Ariotti, Silvia; Costa, Catarina; Nunes-Cabaço, HelenaSARS-CoV-2 has emerged as a human pathogen, causing clinical signs, from fever to pneumonia-COVID-19-but may remain mild or asymptomatic. To understand the continuing spread of the virus, to detect those who are and were infected, and to follow the immune response longitudinally, reliable and robust assays for SARS-CoV-2 detection and immunological monitoring are needed. We quantified IgM, IgG, and IgA antibodi...
Dominance of vaccine serotypes in pediatric invasive pneumococcal infections in...
Silva-Costa, Catarina; Brito, Maria J.; Aguiar, Sandra I.; Lopes, Joana P.; Ramirez, Mário; Melo Cristino, JoséWe evaluated the impact of continued 13-valent pneumococcal conjugate vaccine (PCV13) use in the private market (uptake of 61%) in pediatric invasive pneumococcal disease (pIPD) in Portugal (2012-2015). The most frequently detected serotypes were: 3 (n = 32, 13.8%), 14 (n = 23, 9.9%), 1 (n = 23, 9.9%), 7F (n = 15, 6.4%), 19A (n = 13, 5.6%), 6B and 15B/C (both n = 12, 5.2%), and 24F, 10A and 12B (all with n = 10...
Dominance of vaccine serotypes in pediatric invasive pneumococcal infections in...
Silva-Costa, Catarina; Brito, Maria J.; Aguiar, Sandra I.; Lopes, Joana P.; Ramirez, Mário; Melo-Cristino, JoséWe evaluated the impact of continued 13-valent pneumococcal conjugate vaccine (PCV13) use in the private market (uptake of 61%) in pediatric invasive pneumococcal disease (pIPD) in Portugal (2012–2015). The most frequently detected serotypes were: 3 (n = 32, 13.8%), 14 (n = 23, 9.9%), 1 (n = 23, 9.9%), 7F (n = 15, 6.4%), 19A (n = 13, 5.6%), 6B and 15B/C (both n = 12, 5.2%), and 24F, 10A and 12B (all with n = 10...
Conjugate vaccine serotypes persist as major causes of non-invasive pneumococca...
Horácio, Andreia; Silva-Costa, Catarina; Lopes, Elísia; Ramirez, Mário; Cristino, José MeloNon-invasive pneumococcal pneumonia (NIPP) is a frequent cause of morbidity and mortality worldwide. The 13-valent pneumococcal conjugate vaccine (PCV13) was included in the national immunization program of children living in Portugal in 2015. Until then, PCV7 (since late 2001) and PCV13 (since early 2010) were given through the private market. We determined the serotype distribution and antimicrobial susceptib...