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Molecular Patterns and Antimicrobial Resistance Characterization of Salmonella ...
Francisco, Moisés; Belas, Adriana; Costa, Sofia Santos; Menezes, Juliana; Ramos, Jorge; Couto, Isabel; Viveiros, Miguel; Pomba, ConstançaThis research was supported by Fundação para a Ciência e a Tecnologia (FCT, Portugal) through funds to GHTM—UIDP/04413/2020, LA-REAL—LA/P/0117/2020, CIISA and AL4AnimalS (UIDB/00276/2020 and LA/P/0059/2020, respectively). J.M. was supported by a PhD fellowship (2020.07562.BD). S.S.C. was supported by FCT through CEECINST/00042/2021/CP1773/CT0009 (https://doi.org/10.54499/CEECINST/00042/2021/CP1773/CT0009).; The...
Integrating informatics tools and portable sequencing technology for rapid dete...
Phelan, Jody E.; O'Sullivan, Denise M.; Machado, Diana; Ramos, Jorge; Oppong, Yaa E.A.; Campino, Susana; O'Grady, Justin; McNerney, RuthBackground: Mycobacterium tuberculosis resistance to anti-tuberculosis drugs is a major threat to global public health. Whole genome sequencing (WGS) is rapidly gaining traction as a diagnostic tool for clinical tuberculosis settings. To support this informatically, previous work led to the development of the widely used TBProfiler webtool, which predicts resistance to 14 drugs from WGS data. However, for accur...
CPLP-TB:
Perdigão, João; Silva, Carla; Diniz, Jaciara; Pereira, Catarina; Machado, Diana; Ramos, Jorge; Silva, Hugo; Abilleira , Fernanda; Brum, ClariceA Tuberculose (TB) permanece um grave problema de saúde pública na Comunidade dos Países de Língua Portuguesa (CPLP). Apesar da ampla variância da incidência da TB nos seus estados-membro e de um fluxo migratório contínuo entre os países que integram este grupo, existe uma enorme lacuna no que diz respeito ao conhecimento da estrutura populacional conjunta do Mycobacterium tuberculosis e circulação de estirpes ...
Genetic diversity, transmission dynamics and drug resistance of Mycobacterium t...
Perdigão, João; Clemente, Sofia; Ramos, Jorge; Masakidi, Pedro; Machado, Diana; Silva, Carla; Couto, Isabel; Viveiros, Miguel; Taveira, NunoThe authors wish to thank the laboratory, clinical, and nursing staff of HDP, whose high quality of service and cooperation have made this study possible. Financial support was provided by the Fundacao para a Ciencia e a Tecnologia (FCT) Portugal [PTDC/SAU-EPI/122400/2010], part of the EDCTP2 program supported by the European Union. JP was supported by a post doc fellowship from project [PTDC/SAU-EPI/122400/201...
The variability and reproducibility of whole genome sequencing technology for d...
Phelan, Jody; O'Sullivan, Denise M.; Machado, Diana; Ramos, Jorge; Whale, Alexandra S.; O'Grady, Justin; Dheda, Keertan; Campino, Susana; McNerney, RuthBackground: The emergence of resistance to anti-tuberculosis drugs is a serious and growing threat to public health. Next-generation sequencing is rapidly gaining traction as a diagnostic tool for investigating drug resistance in Mycobacterium tuberculosis to aid treatment decisions. However, there are few little data regarding the precision of such sequencing for assigning resistance profiles. Methods: We inve...
Direct detection by the xpert MTB/RIF assay and characterization of multi and p...
Rabna, Paulo; Ramos, Jorge; Ponce, Gema; Sanca, Lilica; Mané, Morto; Armada, Ana; Machado, Diana; Vieira, Fina; Gomes, Victor F.; Martins, ElisabetePMID:26017968 WOS:000355185600088; Background This study aimed to evaluate the usefulness of the Xpert MTB/RIF assay for the rapid direct detection of M. tuberculosis complex (MTBC) strains and rifampicin resistance associated mutations in a resource-limited setting such as Guinea-Bissau and its implications in the management of tuberculosis (TB) and drug resistant tuberculosis, complementing the scarce informa...
Assessment of the BD MGIT TBc identification test for the detection of mycobact...
Machado, Diana; Ramos, Jorge; Couto, Isabel; Cadir, Nureisha; Narciso, Inácio; Coelho, Elizabeth; Viegas, Sofia; Viveiros, MiguelWOS:000330695600001; We evaluate the performance of the TBcID assay in a panel of 100 acid-fast bacilli cultures. Sixty-four isolates were TBcID positive for Mycobacterium tuberculosis complex (MTBC), whereas 36 gave negative results. These included 28 nontuberculous mycobacteria, one nonmycobacterial isolate, one M. tuberculosis, and six M. bovis BCG strains. This corresponds to a sensitivity of 90.14%, specif...
Ethidium bromide transport across Mycobacterium smegmatis cell-wall: correlatio...
Rodrigues , Liliana; Ramos, Jorge; Couto, Isabel Maria dos Santos Leitão; Amaral, Leonard; Bettencourt, Miguel ViveirosBackground Active efflux systems and reduced cell-wall permeability are considered to be the main causes of mycobacterial intrinsic resistance to many antimicrobials. In this study, we have compared the Mycobacterium smegmatis wild-type strain mc2155 with knockout mutants for porins MspA (the main porin of M. smegmatis) and MspC, the efflux pump LfrA (the main efflux pump system of M. smegmatis) and its repress...
pH Modulation of efflux pump activity of multi-drug resistant Escherichia coli ...
Martins, Ana; Spengler, Gabriella; Rodrigues, Liliana; Viveiros, Miguel; Ramos, Jorge; Martins, Marta; Couto, Isabel; Fanning, Séamus; Pagès, Jean MarieBACKGROUND: Resistance Nodulation Division (RND) efflux pumps of Escherichia coli extrude antibiotics and toxic substances before they reach their intended targets. Whereas these pumps obtain their energy directly from the proton motive force (PMF), ATP-Binding Cassette (ABC) transporters, which can also extrude antibiotics, obtain energy from the hydrolysis of ATP. Because E. coli must pass through two pH dist...