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Genome-wide association study identifies genetic variants underlying footrot in...

Gaspar, Daniel; Ginja, Catarina; Carolino, Nuno; Leão, Célia; Monteiro, Helena; Tábuas, Lino; Branco, Sandra; Padre, Ludovina; Caetano, Pedro

This is the first attempt to identify molecular markers associated with footrot in Portuguese Merino sheep. These findings provide relevant information on a likely genetic association underlying footrot resistance/susceptibility and the potential candidate genes affecting this trait. Genetic selection strategies assisted on the information obtained from this study could enhance Merino sheep-breeding programs, i...


A metagenomics approach to characterize the footrot microbiome in Merino sheep

Usié, Ana; Leão, Célia; Gaspar, Daniel; Monteiro, Helena; Tábuas, Lino; Bettencourt, Elisa; Caetano, Pedro; Padre, Ludovina; Carolino, Nuno

In the Portuguese Alentejo region, Merino sheep breed is the most common breed, reared for the production of meat, dairy, and wool. Footrot is responsible for lameness, decreased animal welfare, and higher production losses, generating a negative economic impact. The disease is caused by Dichelobacter nodosus that interacts with the sheep foot microbiome, to date largely uncharacterized. In fact, Dichelobacter ...


An improved reference genome and first organelle genomes of Quercus suber

Usié, Ana; Serra, Octávio; Barros, Pedro M.; Barbosa, Pedro; Leão, Célia; Capote, Tiago; Almeida, Tânia; Rodrigues, Leandra; Carrasquinho, Isabel

Cork oak (Quercus suber L.) is an ecologically and economically important evergreen tree species native to the Mediterranean region and widespread in southwest Europe and northwest Africa. An improved genome assembly of cork oak using a combination of Illumina and PacBio sequencing is presented in this study. The assembled genome contains 2351 scaffolds longer than 1000 bp, accounting for 765.7 Mbp of genome si...


Kinship Analysis and Pedigree Reconstruction of a Natural Regenerated Cork Oak ...

Mendes, Bruna; Sampaio, Teresa; Antunes, Marta; Magalhães, Hugo; Costa e Silva, Filipe; Borges, Carla; Simões, Fernanda; Usié, Ana

Cork oak (Quercus suber L.) is a valuable forest species in the western Mediterranean Basin due to its ecological value and the production of cork (a renewable natural material). Cork quality depends on the genetic background and cork oak environment, which has long been recognized. As no cork oak genetic trials with pedigree information were available, the inference of the genetic relatedness between individua...


Kinship Analysis and Pedigree Reconstruction of a Natural Regenerated Cork Oak ...

Mendes, Bruna; Sampaio, Teresa; Antunes, Marta A.; Magalhães, Hugo; Silva, Filipe Costa; Borges, Carla; Simões, Fernanda; Usié, Ana

Cork oak (Quercus suber L.) is a valuable forest species in the western Mediterranean Basin due to its ecological value and the production of cork (a renewable natural material). Cork quality depends on the genetic background and cork oak environment, which has long been recognized. As no cork oak genetic trials with pedigree information were available, the inference of the genetic relatedness between individua...


The crown pearl: a draft genome assembly of the European freshwater pearl musse...

Gomes-dos-Santos, André; Lopes-Lima, Manuel; Machado, André M.; Ramos, António Marcos; Usié, Ana; Bolotov, Ivan N.; Vikhrev, Ilya V.; Breton, Sophie

Since historical times, the inherent human fascination with pearls turned the freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758) into a highly valuable cultural and economic resource. Although pearl harvesting in M. margaritifera is nowadays residual, other human threats have aggravated the species conservation status, especially in Europe. This mussel presents a myriad of rare biological feat...

Date: 2021   |   Origin: Biblioteca Digital do IPB

Comparative transcriptomic response of two Pinus species to infection with the ...

Gaspar, Daniel; Trindade, Cândida; Usié, Ana; Meireles, Brigida; Fortes, Ana Margarida; Guimarães, Joana Bagoin; Simões, Fernanda; Costa, Rita Lourenço

Pine wilt disease (PWD) caused by pine wood nematode (PWN), Bursaphelenchus xylophilus, is a serious threat to global forest populations of conifers, in particular Pinus spp. Recently, the presence of PWN was reported in dead Yunnan pine (Pinus yunnanensis) trees under natural conditions. To further understand the potential impact caused by PWN in Yunnan pine populations, a transcriptional profiling analysis wa...


The draft genome sequence of cork oak

Ramos, António Marcos; Usié, Ana; Barbosa, Pedro; Barros, Pedro M.; Capote, Tiago; Chaves, Inês; Simões, Fernanda; Abreu, Isabl; Carrasquinho, Isabel

Cork oak (Quercus suber) is native to southwest Europe and northwest Africa where it plays a crucial environmental and economical role. To tackle the cork oak production and industrial challenges, advanced research is imperative but dependent on the availability of a sequenced genome. To address this, we produced the first draft version of the cork oak genome. We followed a de novo assembly strategy based on hi...


ChlP-Seq reveals that QsMYB1 directly targets genes involved in lignin and sube...

Capote, Tiago; Barbosa, Pedro; Usié, Ana; Ramos, António Marcos; Inácio, Vera; Ordás, Ricardo; Gonçalves, Sónia; Morais-Cecilio, Leonor

Background: Gene activity is largely controlled by transcriptional regulation through the action of transcription factors and other regulators. QsMYB1 is a member of the R2R3-MYB transcription factor family related to secondary growth, and in particular, with the cork development process. In order to identify the putative gene targets of QsMYB1 across the cork oak genome we developed a ChIP-Seq strategy. Result...


The draft genome sequence of cork oak

Ramos, António Marcos; Usié, Ana; Barbosa, Pedro; Barros, Pedro M.; Capote, Tiago; Chaves, Inês; Simões, Fernanda; Abreu, Isabl; Carrasquinho, Isabel

Cork oak (Quercus suber) is native to southwest Europe and northwest Africa where it plays a crucial environmental and economical role. To tackle the cork oak production and industrial challenges, advanced research is imperative but dependent on the availability of a sequenced genome. To address this, we produced the first draft version of the cork oak genome. We followed a de novo assembly strategy based on hi...


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