19 documents found, page 1 of 2
High-value brown algae extracts using deep eutectic solvents and microwave-assi...
Jesus, Meirielly; Romaní, Aloia; Santos, Joana; Pires, Preciosa; Del-Río, Pablo; Mata, Fernando; Fernandes, Élia; Ramos, Carla; Vaz-Velho, ManuelaUtilizing deep eutectic solvents (DESs) combined with microwave-assisted extraction (MAE) provides a sustainable method for extracting bioactive compounds from the macroalgae Ascophyllum nodosum and Laminaria hyperborea. Two DES formulations, choline chloride/lactic acid (ChCl/LA) and sodium acetate/lactic acid (AcNa/LA), were evaluated under varying extraction conditions. For L. hyperborea, ChCl/LA at 150 °C f...
The matrix effect in the RT-PCR detection of SARS-CoV-2 using saliva without RN...
Morais, Orlando; Alves, Manuel Rui; Ramos, Carla; Ferreira, Fernando; Fernandes, PauloThe present work focuses on the detection of SARS-CoV-2 in saliva, contributing to understanding the inhibition effect of the matrix and its influence on the results. Detection of viral genes ORF1ab, N, and E was performed by RT-PCR using saliva directly in the reaction without RNA extraction. Different amounts of saliva were spiked with increasing amounts of viral RNA from COVID-19 patients and subjected to RT...
Virulence potential and antibiotic susceptibility of S. aureus strains isolated...
Fernandes, Adriana; Ramos, Carla; Monteiro, Vítor; Santos, Joana; Fernandes, PauloStaphylococcus spp. are common members of the normal human flora. However, some Staphylococcus species are recognised as human pathogens due to the production of several virulence factors and enterotoxins that are particularly worrisome in food poisoning. Since many of Staphylo-coccal food poisoning outbreaks are typically associated with cross-contamination, the detection of S. aureus on food handlers was perf...
Envelhecer na perspetiva psicológica e social: promoção da saúde, qualidade de ...
Ramos, Carla; Gil, Katherine; Sousa, Marta; Fernandes, Patrícia; Teixeira, Rubina; Faria, Ana LúciaAtualmente, o autocuidado, a prevenção na saúde e a melhoria da qualidade de vida de cada indivíduo, têm levantado várias questões no que diz respeito à saúde no envelhecimento, pois a esperança média de vida aumentou, e com isso surgiu a necessidade de melhorar os cuidados nesta faixa etária. Com o avanço da tecnologia e com o aumento da literacia digital junto das pessoas mais velhas, vão surgindo novos métod...
Performance of saliva as a specimen to detect SARS-CoV-2
Fernandes, Paulo; Ferreira, Fernando Alberto da Conceição; Morais, Orlando; Ramos, Carla; Fernandes, Élia; Rocha, Susana; Rocha, Rui Jorge AlvesMassive testing to detect SARS-CoV-2 is an imperious need in times of epidemic but also presents challenges in terms of its concretization. The use of saliva as an alternative to nasopharyngeal swabs (NPS) has advantages, being more friendly to the patient and not requiring trained health workers, so much needed in other functions. This study used a total of 452 dual samples (saliva and NPS) of patients suspect...
Ambient air waste sorting facilities could be a source of antibiotic resistant ...
Calheiros, Ana; Santos, Joana; Ramos, Carla; Vasconcelos, Marta; Fernandes, PauloThe antimicrobial resistance of Staphylococcus spp. and Gram negative strains present in air samples from waste sorting facilities was assessed. Phenotypic studies have revealed a high percentage of strains of Staphylococcus spp. resistant to methicillin. Genotypically and by RT-PCR, it was found that the mecA gene usually associated with methicillin resistance was present in 8% of the Staphylococcus strains is...
Inhibitory effects of Pinus pinaster Aiton subsp. atlantica bark extracts again...
Barros, Diana; Vieito, Catarina; Santos, Joana; Ramos, Carla; Vaz-Velho, ManuelaPine bark extracts are a rich source of natural polyphenols that besides their known antioxidant activity may also exhibit significant antimicrobial properties. In the current study, the antimicrobial activity of Pinus pinaster Aiton subsp. atlantica extracts was screened against gram-positive bacteria (Bacillus cereus, Clostridium perfringens, Listeria monocytogenes, Staphylococcus aureus), gram-negative bacte...
Unravelling the biological potential of Pinus pinaster bark extracts
Santos, Pedro Miguel Ferreira; Genisheva, Zlatina; Botelho, C. M.; Santos, Joana; Ramos, Carla; Teixeira, J. A.; Rocha, Cristina M. R.Natural compounds from agro-food by-products have fostered interest in food industries. The aim of this study was to unravel potential uses for Pinus pinaster bark extracts (PBE). As functional features of this type of extracts are usually attributed to phenolic compounds, the extraction process was studied. Different PBEs were achieved, with high content in phenolic compounds, using different water/ethanol com...
Lactobacillus plantarum isolated from cheese: production and partial characteri...
Nero, Luis Augusto; Fernandes, Paulo; Loureiro, Daniela; Monteiro, Vitor; Ramos, Carla; Todorov, Svetoslav Dimitrov; Guerreiro, Joana SantosLactobacillus plantarum B391, a strain isolated from an artisanal French cheese, is a producer of a bacteriocin, expressing activity against Enterococcus faecalis NCTC 775, Clostridium perfringens NCTC 13170 and several Listeria monocytogenes strains. High stability was recorded after heat treatment at 121 °C for 20 min and when stored at 4 °C for more than 40 days. A challenge test performed in milk for 11 day...
Lactobacillus plantarum isolated from cheese: Production and partial characteri...
Fernandes, Paulo; Loureiro, Daniela; Monteiro, Vítor; Ramos, Carla; Nero, Luís Augusto; Todorov, Svetoslav Dimitrov; Santos, JoanaLactobacillus plantarum B391, a strain isolated from an artisanal French cheese, is a producer of a bacteriocin, expressing activity against Enterococcus faecalis NCTC 775, Clostridium perfringens NCTC 13170 and several Listeria monocytogenes strains. High stability was recorded after heat treatment at 121 °C for 20 min and when stored at 4 °C for more than 40 days. A challenge test performed in milk for 11 day...