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Structural and enzymatic characterization of Peruvianin‑I, the first germin-lik...
Cruz, Wallace T. da; Bezerra, Eduardo H. S.; Grangeiro, Thalles B.; Lopes, Jose L. S.; Silva, Maria Z. R.; Ramos, Márcio Viana; Rocha, Bruno A. M.The germin-like protein (GLP) purified from Thevetia peruviana, Peruvianin-I, is the only one described as having proteolytic activity. Therefore, the goal of this study was to investigate the structural features responsible for its enzymatic activity. Although the amino acid sequence of Peruvianin-I showed high identity with other GLPs, it exhibited punctual mutations, which were responsible for the absence of...
Identification, characterization, and antifungal activity of cysteine peptidase...
Freitas, Cleverson D. T.; Silva, Rafaela O; Ramos, Márcio Viana; Porfírio, Camila T. M. N.; Farias, Davi F.; Sousa, Jeanlex S; Oliveira, João P. B.Cysteine peptidases (EC 3.4.22) are the most abundant enzymes in latex fluids. However, their physiological functions are still poorly understood, mainly related to defense against phytopathogens. The present study reports the cDNA cloning and sequencing of five undescribed cysteine peptidases from Calotropis procera (Aiton) Dryand (Apocynaceae) as well as some in silico analyses. Of these, three cysteine pepti...
Biotechnological potential of a cysteine protease (CpCP3) from Calotropis proce...
Silva, Maria Z. R.; Oliveira, João P. B.; Ramos, Márcio Viana; Farias, Davi F.; Sá, Chayenne A. de; Ribeiro, Juliana A. C.; Silva, Ayrles F. B.This article reports the characterization and evaluation of the biotechnological potential of a cysteine protease purified from Calotropis procera (CpCP3). This enzyme was highly stable to different metal ions and was able to hydrolyze κ-casein similarly to bovine chymosin. Atomic force microscopy showed that the process of casein micelle aggregation induced by CpCP3 was similar to that caused by chymosin. The ...
Crystal structure of an antifungal osmotin-like protein from Calotropis procera...
Ramos, Márcio Viana; Oliveira, Raquel S. B. de; Pereira, Humberto M.; Moreno, Frederico B. M. B.; Lobo, Marina D. P.; Rebelo, Luciana MagalhãesCpOsm is an antifungal osmotin/thaumatin-like protein purified from the latex of Calotropis procera. The protein is relatively thermostable and retains its antifungal activity over a wide pH range; therefore, it may be useful in the development of new antifungal drugs or transgenic crops with enhanced resistance to phytopathogenic fungi. To gain further insight into the mechanism of action of CpOsm, its three-d...
Insights on the inhibition properties of Jatromollistatin (a cyclic heptapeptid...
Jucá, Thiago Lustosa; Ramos, Márcio Viana; Cilli, Eduardo Maffud [UNESP]; Neto, Antônio Eufrásio Vieira; Mackessy, Stephen P.Made available in DSpace on 2022-04-29T08:40:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01; Jatropha mollissima is endemic to Brazil and is used for traditional medicinal purposes, including the treatment of snakebite. In this study, latex obtained from this plant was fractioned using reversed-phase chromatography, and the fractions were then screened for peptides. A 755 g/mol peptide was obta...
Latex proteins from Calotropis procera: toxicity and immunological tolerance re...
Bezerra, Camila F.; Mota, Érika F.; Silva, Ana Claudia M.; Tomé, Adriana R.; Silva, Maria Z. R.; Brito, Daniel de; Porfírio, Camila T. M. N.Many thousands of plants are disseminated worldwide in traditional and folk medicines based on the belief that their leaves, roots, seeds, bark or secretions, when adequately handled, can treat, alleviate or ameliorate numerous disease symptoms. Calotropis procera (Apocynaceae) is a popular medicinal plant and the claims of this shrub's phytomedicinal properties have been scientifically validated. In this study...
In vitro tissue culture of the medicinal shrub Calotropis procera to produce ph...
Teixeira, Fabiano M.; Ramos, Márcio Viana; Soares, Arlete A.; Oliveira, Raquel S. B.; Almeida-Filho, Luiz Carlos P.; Oliveira, Jefferson S.The latex of Calotropis procera is a rich source of proteins that have anti-inflammatory, anti-nociceptive and selective cytotoxic and anti-tumorigenic properties. In this study, two distinct protocols were devel oped to culture C. procera in vitro in order to obtain active molecules. Soluble proteins of both callus and root cultures were extracted and tested for various activities found in the latex. Anti-infl...
Seeds of Amazonian Fabaceae as a source of new lectins
Fernandes, Andréia Varmes; Ramos, Márcio Viana; Gonçalves, José Francisco Carvalho de; Maranhão, Paulo Abraão C.; Chevreuil, Larissa RamosSeeds from fifty native Amazonian Fabaceae species (representing subfamilies Caesalpinioideae, Mimosoideae and Faboideae) were screened for the presence of new lectins. Their crude protein extracts were assayed for hemagglutinating activity (HA). The protein fractions of Anadenanthera peregrina, Dimorphandra caudata, Ormosia lignivalvis and Swartzia laevicarpa exhibited HA, and this activity was inhibited by ga...
Fungicidal properties and insights on the mechanisms of the action of volatile ...
Souza, Diego Pereira; Pimentel, Renah Boanerges de Queiroz; Santos, Alberdan Silva; Albuquerque, Patrícia Melchionna; Fernandes, Andréia VarmesThe Amazonian Aniba species are world-renowned for their essential oils (EOs). The molecules derived from EOs have been intensively investigated in regards to their potential for disease control in plants. The aim of this study was to investigate the antifungal properties of Aniba canelilla EO (ACEO) and Aniba parviflora EO (APEO) when used against eight phytopathogenic fungi. Gas chromatography-mass spectromet...
Lectin genes and their mature proteins: Still an exciting matter, as revealed b...
Fernandes, Andréia Varmes; Ramos, Márcio Viana; Costa, José Hélio; Vasconcelos, Ilka Maria Aria; MOREIRA, Renato A.Two new lectins were purified through affinity chromatography after crude extract preparation under high ionic strength. The hemagglutinating activity of these lectins from the seeds of the legumes Dioclea bicolor (DBL) and Deguelia scandens (DSL) was inhibited by galactose and glucose, respectively, and the molecular masses were estimated at 24 and 22kDa (via SDS-PAGE), respectively. The alignment of internal ...